Histological Comparison of two Special Methods of Staining Melanin in Human Skin

SUMMARY: Human skin melanin was stained using the Fontana’s silver nitrate method and Schmorl method. The results showed that, in the Fontana’s silver nitrate method, melanin and silver-bound cells were black and other tissues were red. When stained using the Schmorl method, effe...

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Autores principales:	Shataer,Munire, Shataer,Saimire, Libin,Liao, Tian,Li, Shengbin,Bai
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Melanin Fontana's Silver Nitrate Method Schmorl Method
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601535
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spelling	oai:scielo:S0717-950220200006015352020-10-30Histological Comparison of two Special Methods of Staining Melanin in Human SkinShataer,MunireShataer,SaimireLibin,LiaoTian,LiShengbin,Bai Melanin Fontana's Silver Nitrate Method Schmorl Method SUMMARY: Human skin melanin was stained using the Fontana’s silver nitrate method and Schmorl method. The results showed that, in the Fontana’s silver nitrate method, melanin and silver-bound cells were black and other tissues were red. When stained using the Schmorl method, effects on melanin differed based on whether the nuclei were stained. When the nucleus was stained, melanin appeared blue-black or blue-green, and other tissue structures were purple. When the nucleus was not stained, melanin was orange and other structures were pink. Comparing the two staining methods, we concluded that Fontana’s silver nitrate method takes a long time; in contrast, the Schmorl method showed two different types of results depending on whether the nucleus was stained, and it takes less time than Fontana staining, so we here consider the Schmorl method more suitable for special staining of melanin than Fontana’s silver nitrate method.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.6 20202020-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601535en10.4067/S0717-95022020000601535
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Melanin Fontana's Silver Nitrate Method Schmorl Method
spellingShingle	Melanin Fontana's Silver Nitrate Method Schmorl Method Shataer,Munire Shataer,Saimire Libin,Liao Tian,Li Shengbin,Bai Histological Comparison of two Special Methods of Staining Melanin in Human Skin
description	SUMMARY: Human skin melanin was stained using the Fontana’s silver nitrate method and Schmorl method. The results showed that, in the Fontana’s silver nitrate method, melanin and silver-bound cells were black and other tissues were red. When stained using the Schmorl method, effects on melanin differed based on whether the nuclei were stained. When the nucleus was stained, melanin appeared blue-black or blue-green, and other tissue structures were purple. When the nucleus was not stained, melanin was orange and other structures were pink. Comparing the two staining methods, we concluded that Fontana’s silver nitrate method takes a long time; in contrast, the Schmorl method showed two different types of results depending on whether the nucleus was stained, and it takes less time than Fontana staining, so we here consider the Schmorl method more suitable for special staining of melanin than Fontana’s silver nitrate method.
author	Shataer,Munire Shataer,Saimire Libin,Liao Tian,Li Shengbin,Bai
author_facet	Shataer,Munire Shataer,Saimire Libin,Liao Tian,Li Shengbin,Bai
author_sort	Shataer,Munire
title	Histological Comparison of two Special Methods of Staining Melanin in Human Skin
title_short	Histological Comparison of two Special Methods of Staining Melanin in Human Skin
title_full	Histological Comparison of two Special Methods of Staining Melanin in Human Skin
title_fullStr	Histological Comparison of two Special Methods of Staining Melanin in Human Skin
title_full_unstemmed	Histological Comparison of two Special Methods of Staining Melanin in Human Skin
title_sort	histological comparison of two special methods of staining melanin in human skin
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601535
work_keys_str_mv	AT shataermunire histologicalcomparisonoftwospecialmethodsofstainingmelanininhumanskin AT shataersaimire histologicalcomparisonoftwospecialmethodsofstainingmelanininhumanskin AT libinliao histologicalcomparisonoftwospecialmethodsofstainingmelanininhumanskin AT tianli histologicalcomparisonoftwospecialmethodsofstainingmelanininhumanskin AT shengbinbai histologicalcomparisonoftwospecialmethodsofstainingmelanininhumanskin
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Histological Comparison of two Special Methods of Staining Melanin in Human Skin

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