Altered Oligodendrocytes in Spinal Enlargements of Streptozotocin Diabetic Rats

SUMMARY: Disturbances of sensory and motor nerve conduction velocity in the spinal cord as well as degenerated myelin sheaths are observed in diabetic patients and animal models. Indeed, oligodendrocytes (OLs), which are important neuroglial cells, generate myelin in the central nervous system. Spin...

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Autores principales:	Baimai,Sani, Janta,Sirorat, Lanlua,Passara, Chookliang,Amornrat, Niyomchan,Apichaya, Sricharoenvej,Sirinush
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Spinal enlargement Oligodendrocyte Myelin Diabetes mellitus Streptozotocin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601606
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spelling	oai:scielo:S0717-950220200006016062020-10-30Altered Oligodendrocytes in Spinal Enlargements of Streptozotocin Diabetic RatsBaimai,SaniJanta,SiroratLanlua,PassaraChookliang,AmornratNiyomchan,ApichayaSricharoenvej,Sirinush Spinal enlargement Oligodendrocyte Myelin Diabetes mellitus Streptozotocin SUMMARY: Disturbances of sensory and motor nerve conduction velocity in the spinal cord as well as degenerated myelin sheaths are observed in diabetic patients and animal models. Indeed, oligodendrocytes (OLs), which are important neuroglial cells, generate myelin in the central nervous system. Spinal enlargement, including cervical and lumbar enlargements, innervates all limbs. Thus, the purposes of this study were to examine and compare the ultrastructural alterations of OLs in spinal enlargements of streptozotocin (STZ)- induced diabetic rats and controls. Thirteen male Sprague-Dawley rats were induced with STZ in citrate buffer and six control rats were injected with the same buffer solution. All rats were sacrificed after inductions at four (short-term DM) and twenty-four weeks (long-term DM). The selected spinal enlargements were processed for transmission electron microscopy. The OL alterations in both the cervical and lumbar enlargements were apparently the same. In short-term DM, the nuclei of OLs became swelled with chromatin clumping. Cytoplasmic organelles were moderately damaged. In long-term DM, OLs contained shrinkage nuclei with thick heterochromatin clumping. Severely degenerated mitochondria with disrupted cristae and broken membranes were observed. Moreover, distended and fragmented rough endoplasmic reticulum were observed, and large clear areas were present in the cytoplasm. Additionally, the loosening, splitting, and destruction of myelin lamellae were found. This study can provide important preliminary information about the alteration of OLs in the spinal cords of diabetic patients, which might be involve in the impairments of sensory and motor conduction velocities in these individuals.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.6 20202020-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601606en10.4067/S0717-95022020000601606
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Spinal enlargement Oligodendrocyte Myelin Diabetes mellitus Streptozotocin
spellingShingle	Spinal enlargement Oligodendrocyte Myelin Diabetes mellitus Streptozotocin Baimai,Sani Janta,Sirorat Lanlua,Passara Chookliang,Amornrat Niyomchan,Apichaya Sricharoenvej,Sirinush Altered Oligodendrocytes in Spinal Enlargements of Streptozotocin Diabetic Rats
description	SUMMARY: Disturbances of sensory and motor nerve conduction velocity in the spinal cord as well as degenerated myelin sheaths are observed in diabetic patients and animal models. Indeed, oligodendrocytes (OLs), which are important neuroglial cells, generate myelin in the central nervous system. Spinal enlargement, including cervical and lumbar enlargements, innervates all limbs. Thus, the purposes of this study were to examine and compare the ultrastructural alterations of OLs in spinal enlargements of streptozotocin (STZ)- induced diabetic rats and controls. Thirteen male Sprague-Dawley rats were induced with STZ in citrate buffer and six control rats were injected with the same buffer solution. All rats were sacrificed after inductions at four (short-term DM) and twenty-four weeks (long-term DM). The selected spinal enlargements were processed for transmission electron microscopy. The OL alterations in both the cervical and lumbar enlargements were apparently the same. In short-term DM, the nuclei of OLs became swelled with chromatin clumping. Cytoplasmic organelles were moderately damaged. In long-term DM, OLs contained shrinkage nuclei with thick heterochromatin clumping. Severely degenerated mitochondria with disrupted cristae and broken membranes were observed. Moreover, distended and fragmented rough endoplasmic reticulum were observed, and large clear areas were present in the cytoplasm. Additionally, the loosening, splitting, and destruction of myelin lamellae were found. This study can provide important preliminary information about the alteration of OLs in the spinal cords of diabetic patients, which might be involve in the impairments of sensory and motor conduction velocities in these individuals.
author	Baimai,Sani Janta,Sirorat Lanlua,Passara Chookliang,Amornrat Niyomchan,Apichaya Sricharoenvej,Sirinush
author_facet	Baimai,Sani Janta,Sirorat Lanlua,Passara Chookliang,Amornrat Niyomchan,Apichaya Sricharoenvej,Sirinush
author_sort	Baimai,Sani
title	Altered Oligodendrocytes in Spinal Enlargements of Streptozotocin Diabetic Rats
title_short	Altered Oligodendrocytes in Spinal Enlargements of Streptozotocin Diabetic Rats
title_full	Altered Oligodendrocytes in Spinal Enlargements of Streptozotocin Diabetic Rats
title_fullStr	Altered Oligodendrocytes in Spinal Enlargements of Streptozotocin Diabetic Rats
title_full_unstemmed	Altered Oligodendrocytes in Spinal Enlargements of Streptozotocin Diabetic Rats
title_sort	altered oligodendrocytes in spinal enlargements of streptozotocin diabetic rats
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601606
work_keys_str_mv	AT baimaisani alteredoligodendrocytesinspinalenlargementsofstreptozotocindiabeticrats AT jantasirorat alteredoligodendrocytesinspinalenlargementsofstreptozotocindiabeticrats AT lanluapassara alteredoligodendrocytesinspinalenlargementsofstreptozotocindiabeticrats AT chookliangamornrat alteredoligodendrocytesinspinalenlargementsofstreptozotocindiabeticrats AT niyomchanapichaya alteredoligodendrocytesinspinalenlargementsofstreptozotocindiabeticrats AT sricharoenvejsirinush alteredoligodendrocytesinspinalenlargementsofstreptozotocindiabeticrats
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Altered Oligodendrocytes in Spinal Enlargements of Streptozotocin Diabetic Rats

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