Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model

SUMMARY: Previous studies from our group described the consequences of using ethanol on penile erection. Nevertheless, the molecular mechanisms surrounding microRNAs, apoptosis process and their relationship with erectile dysfunction associated with alcohol consumption are still poorly understood. T...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sarraipo,Vagner Schiavoni, Lizarte Neto,Fermino Sanches, Carvalho,Camila Albuquerque Mello de, Silva,Jairo Pinheiro da, Durand,Marina Toledo, Schimming,Bruno Cezar, Martinez,Marcelo, Galli,Maria de Fátima Tazima Sorita, Buono,Fabianna de Oliveira, Tiezzi,Maria Fernanda Barbirato da Mata, Novais,Paulo Cezar, Gula,Isabella Stracieri, Thomazini,José Antônio, Fazan,Valeria Paula Sassoli, Rodrigues,Andressa Romualdo, Tirapelli,Daniela Pretti da Cunha, Cirino,Mucio Luiz de Assis, Molina,Carlos Augusto Fernandes, Junior,Silvio Tucci, Tirapelli,Luis Fernando
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Alcoholism Apoptosis Erectile Dysfunction MicroRNAs
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601639
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022020000601639
record_format	dspace
spelling	oai:scielo:S0717-950220200006016392020-10-30Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism ModelSarraipo,Vagner SchiavoniLizarte Neto,Fermino SanchesCarvalho,Camila Albuquerque Mello deSilva,Jairo Pinheiro daDurand,Marina ToledoSchimming,Bruno CezarMartinez,MarceloGalli,Maria de Fátima Tazima SoritaBuono,Fabianna de OliveiraTiezzi,Maria Fernanda Barbirato da MataNovais,Paulo CezarGula,Isabella StracieriThomazini,José AntônioFazan,Valeria Paula SassoliRodrigues,Andressa RomualdoTirapelli,Daniela Pretti da CunhaCirino,Mucio Luiz de AssisMolina,Carlos Augusto FernandesJunior,Silvio TucciTirapelli,Luis Fernando Alcoholism Apoptosis Erectile Dysfunction MicroRNAs SUMMARY: Previous studies from our group described the consequences of using ethanol on penile erection. Nevertheless, the molecular mechanisms surrounding microRNAs, apoptosis process and their relationship with erectile dysfunction associated with alcohol consumption are still poorly understood. The objective of this analysis was to evaluate the mechanism of apoptosis by the expression of AIF and PARP, as well as their regulatory microRNAs: miR-145, miR-210 and miR-486, in the corpus cavernosum of rats submitted to a semivoluntary alcoholism model. For this study 24 Wistar rats were divided into two groups: control (C) and treated with 20 % ethanol (A) for seven weeks. The corpus cavernosum samples were prepared for immunohistochemical analysis of AIF and PARP protein expression, and microRNAs miR-145, miR-210, miR-486 gene expression in cavernous tissue was performed by real time PCR. The immunohistochemical analysis showed little nuclear positive labeling for the protein PARP and AIF in the corpus cavernosum of control and ethanol treated animals. After analysis of miR-145, -210 and -486 microRNA expression in the 12 animals studied, no results were found with significant statistical difference between the control and alcoholized groups. The expression of AIF and PARP and their regulatory microRNAs involved in apoptotic process (miR-145, miR-210 and miR-486) were not altered in the corpus cavernosum of rats submitted to semivoluntary alcoholism.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.6 20202020-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601639en10.4067/S0717-95022020000601639
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Alcoholism Apoptosis Erectile Dysfunction MicroRNAs
spellingShingle	Alcoholism Apoptosis Erectile Dysfunction MicroRNAs Sarraipo,Vagner Schiavoni Lizarte Neto,Fermino Sanches Carvalho,Camila Albuquerque Mello de Silva,Jairo Pinheiro da Durand,Marina Toledo Schimming,Bruno Cezar Martinez,Marcelo Galli,Maria de Fátima Tazima Sorita Buono,Fabianna de Oliveira Tiezzi,Maria Fernanda Barbirato da Mata Novais,Paulo Cezar Gula,Isabella Stracieri Thomazini,José Antônio Fazan,Valeria Paula Sassoli Rodrigues,Andressa Romualdo Tirapelli,Daniela Pretti da Cunha Cirino,Mucio Luiz de Assis Molina,Carlos Augusto Fernandes Junior,Silvio Tucci Tirapelli,Luis Fernando Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model
description	SUMMARY: Previous studies from our group described the consequences of using ethanol on penile erection. Nevertheless, the molecular mechanisms surrounding microRNAs, apoptosis process and their relationship with erectile dysfunction associated with alcohol consumption are still poorly understood. The objective of this analysis was to evaluate the mechanism of apoptosis by the expression of AIF and PARP, as well as their regulatory microRNAs: miR-145, miR-210 and miR-486, in the corpus cavernosum of rats submitted to a semivoluntary alcoholism model. For this study 24 Wistar rats were divided into two groups: control (C) and treated with 20 % ethanol (A) for seven weeks. The corpus cavernosum samples were prepared for immunohistochemical analysis of AIF and PARP protein expression, and microRNAs miR-145, miR-210, miR-486 gene expression in cavernous tissue was performed by real time PCR. The immunohistochemical analysis showed little nuclear positive labeling for the protein PARP and AIF in the corpus cavernosum of control and ethanol treated animals. After analysis of miR-145, -210 and -486 microRNA expression in the 12 animals studied, no results were found with significant statistical difference between the control and alcoholized groups. The expression of AIF and PARP and their regulatory microRNAs involved in apoptotic process (miR-145, miR-210 and miR-486) were not altered in the corpus cavernosum of rats submitted to semivoluntary alcoholism.
author	Sarraipo,Vagner Schiavoni Lizarte Neto,Fermino Sanches Carvalho,Camila Albuquerque Mello de Silva,Jairo Pinheiro da Durand,Marina Toledo Schimming,Bruno Cezar Martinez,Marcelo Galli,Maria de Fátima Tazima Sorita Buono,Fabianna de Oliveira Tiezzi,Maria Fernanda Barbirato da Mata Novais,Paulo Cezar Gula,Isabella Stracieri Thomazini,José Antônio Fazan,Valeria Paula Sassoli Rodrigues,Andressa Romualdo Tirapelli,Daniela Pretti da Cunha Cirino,Mucio Luiz de Assis Molina,Carlos Augusto Fernandes Junior,Silvio Tucci Tirapelli,Luis Fernando
author_facet	Sarraipo,Vagner Schiavoni Lizarte Neto,Fermino Sanches Carvalho,Camila Albuquerque Mello de Silva,Jairo Pinheiro da Durand,Marina Toledo Schimming,Bruno Cezar Martinez,Marcelo Galli,Maria de Fátima Tazima Sorita Buono,Fabianna de Oliveira Tiezzi,Maria Fernanda Barbirato da Mata Novais,Paulo Cezar Gula,Isabella Stracieri Thomazini,José Antônio Fazan,Valeria Paula Sassoli Rodrigues,Andressa Romualdo Tirapelli,Daniela Pretti da Cunha Cirino,Mucio Luiz de Assis Molina,Carlos Augusto Fernandes Junior,Silvio Tucci Tirapelli,Luis Fernando
author_sort	Sarraipo,Vagner Schiavoni
title	Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model
title_short	Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model
title_full	Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model
title_fullStr	Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model
title_full_unstemmed	Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model
title_sort	expression of aif, parp, and mirnas mir-145, mir-210, and mir-486 associated with apoptosis in the corpus cavernosum of rats subjected to chronic alcoholism model
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601639
work_keys_str_mv	AT sarraipovagnerschiavoni expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT lizartenetoferminosanches expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT carvalhocamilaalbuquerquemellode expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT silvajairopinheiroda expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT durandmarinatoledo expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT schimmingbrunocezar expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT martinezmarcelo expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT gallimariadefatimatazimasorita expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT buonofabiannadeoliveira expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT tiezzimariafernandabarbiratodamata expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT novaispaulocezar expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT gulaisabellastracieri expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT thomazinijoseantonio expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT fazanvaleriapaulasassoli expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT rodriguesandressaromualdo expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT tirapellidanielaprettidacunha expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT cirinomucioluizdeassis expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT molinacarlosaugustofernandes expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT juniorsilviotucci expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel AT tirapelliluisfernando expressionofaifparpandmirnasmir145mir210andmir486associatedwithapoptosisinthecorpuscavernosumofratssubjectedtochronicalcoholismmodel
_version_	1718445172949254144

Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model

Ejemplares similares