Spatio-Temporal Expression Profile of Mettl3/Mettl14 During Mouse Retina Development

SUMMARY: The Mettl3/Mettl14 methyltransferase complex installs the most ubiquitous internal mRNA modification- N6-methyladenosine (m6A). The vertebrate retina development is a multi-step process that requires fine-tuning of multiple cellular events, but very little is known about the potential funct...

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Autores principales:	Zhong,Qi, Guo,Jingyi, Chen,Shuyi
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	m6A methyltransferase Mettl3 Mettl14 Retinogenesis Expression
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601668
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spelling	oai:scielo:S0717-950220200006016682020-10-30Spatio-Temporal Expression Profile of Mettl3/Mettl14 During Mouse Retina DevelopmentZhong,QiGuo,JingyiChen,Shuyi m6A methyltransferase Mettl3 Mettl14 Retinogenesis Expression SUMMARY: The Mettl3/Mettl14 methyltransferase complex installs the most ubiquitous internal mRNA modification- N6-methyladenosine (m6A). The vertebrate retina development is a multi-step process that requires fine-tuning of multiple cellular events, but very little is known about the potential function of Mettl3 and Mettl14 in this process. In this study, we demonstrated the spatio-temporal expression of Mettl3 and Mettl14 during retina development in mouse by quantitative PCR and immunofluorescence staining. We found that these two components of methyltransferase complex could be detected from the beginning of retina development; and the expression of Mettl3 and Mettl14 were gradually restricted to inner nuclear layer (INL) and ganglion cell layer (GCL); Double labeling showed that Mettl3 and Mettl14 had similar expression patterns in mature retinal INL and GCL. Overall, our spatio-temporal expression data provided the foundation for future research on the function of m6A modification in the retina development.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.6 20202020-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601668en10.4067/S0717-95022020000601668
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	m6A methyltransferase Mettl3 Mettl14 Retinogenesis Expression
spellingShingle	m6A methyltransferase Mettl3 Mettl14 Retinogenesis Expression Zhong,Qi Guo,Jingyi Chen,Shuyi Spatio-Temporal Expression Profile of Mettl3/Mettl14 During Mouse Retina Development
description	SUMMARY: The Mettl3/Mettl14 methyltransferase complex installs the most ubiquitous internal mRNA modification- N6-methyladenosine (m6A). The vertebrate retina development is a multi-step process that requires fine-tuning of multiple cellular events, but very little is known about the potential function of Mettl3 and Mettl14 in this process. In this study, we demonstrated the spatio-temporal expression of Mettl3 and Mettl14 during retina development in mouse by quantitative PCR and immunofluorescence staining. We found that these two components of methyltransferase complex could be detected from the beginning of retina development; and the expression of Mettl3 and Mettl14 were gradually restricted to inner nuclear layer (INL) and ganglion cell layer (GCL); Double labeling showed that Mettl3 and Mettl14 had similar expression patterns in mature retinal INL and GCL. Overall, our spatio-temporal expression data provided the foundation for future research on the function of m6A modification in the retina development.
author	Zhong,Qi Guo,Jingyi Chen,Shuyi
author_facet	Zhong,Qi Guo,Jingyi Chen,Shuyi
author_sort	Zhong,Qi
title	Spatio-Temporal Expression Profile of Mettl3/Mettl14 During Mouse Retina Development
title_short	Spatio-Temporal Expression Profile of Mettl3/Mettl14 During Mouse Retina Development
title_full	Spatio-Temporal Expression Profile of Mettl3/Mettl14 During Mouse Retina Development
title_fullStr	Spatio-Temporal Expression Profile of Mettl3/Mettl14 During Mouse Retina Development
title_full_unstemmed	Spatio-Temporal Expression Profile of Mettl3/Mettl14 During Mouse Retina Development
title_sort	spatio-temporal expression profile of mettl3/mettl14 during mouse retina development
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601668
work_keys_str_mv	AT zhongqi spatiotemporalexpressionprofileofmettl3mettl14duringmouseretinadevelopment AT guojingyi spatiotemporalexpressionprofileofmettl3mettl14duringmouseretinadevelopment AT chenshuyi spatiotemporalexpressionprofileofmettl3mettl14duringmouseretinadevelopment
_version_	1718445173907652608

Spatio-Temporal Expression Profile of Mettl3/Mettl14 During Mouse Retina Development

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