Expression of Enamel Proteins in Human Dental Pulp Stem Cells by the Effect of extracellular Matrix

SUMMARY: Mesenchymal stem cells are present in adult tissues such as the human dental pulp. They are pluripotent and can differentiate into various specialized cell types in vitro through appropriate stimuli. Ameloblasts produce human tooth enamel only during embryonic development before tooth erupt...

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Autores principales: Salazar-de-Santiago,Alfredo, Avelar-González,Francisco J, Díaz,Juan Manuel, Campos-Navarro,Paloma M, Flores-Villalpado,Elizz M, Hernández-Cuéllar,Edgar E, Guerrero-Barrera,Alma L
Lenguaje:English
Publicado: Sociedad Chilena de Anatomía 2020
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601742
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spelling oai:scielo:S0717-950220200006017422020-10-30Expression of Enamel Proteins in Human Dental Pulp Stem Cells by the Effect of extracellular MatrixSalazar-de-Santiago,AlfredoAvelar-González,Francisco JDíaz,Juan ManuelCampos-Navarro,Paloma MFlores-Villalpado,Elizz MHernández-Cuéllar,Edgar EGuerrero-Barrera,Alma L Stem cells Dental pulp Extracellular matrix Tissue engineering Dental enamel SUMMARY: Mesenchymal stem cells are present in adult tissues such as the human dental pulp. They are pluripotent and can differentiate into various specialized cell types in vitro through appropriate stimuli. Ameloblasts produce human tooth enamel only during embryonic development before tooth eruption, so endogenous regeneration is not possible. Various efforts have been aimed at generating natural or artificial substitutes for dental enamel with properties similar to the specific components of said tissue. The purpose of this study was to induce human dental pulp stem cells to produce enamel proteins using extracellular matrix derived from the rat tail tendon and pigskin. Primary cultures of human dental pulp stem cells were established and characterized by RT-PCR and immunofluorescence, using mesenchymal cell markers such as CD14, CD40, CD44, CD105, and STRO-1. The cells were then incubated with the extracellular matrix for fourteen days and labeled with specific antibodies to detect the expression of dental enamel proteins such as amelogenin, ameloblastin, enamelisin, tuftelin, and parvalbumin, characteristics of the phenotype of ameloblasts. This work demonstrated a positive effect of the extracellular matrix to induce the expression of enamel proteins in the stem cells of the human dental pulp.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.6 20202020-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601742en10.4067/S0717-95022020000601742
institution Scielo Chile
collection Scielo Chile
language English
topic Stem cells
Dental pulp
Extracellular matrix
Tissue engineering
Dental enamel
spellingShingle Stem cells
Dental pulp
Extracellular matrix
Tissue engineering
Dental enamel
Salazar-de-Santiago,Alfredo
Avelar-González,Francisco J
Díaz,Juan Manuel
Campos-Navarro,Paloma M
Flores-Villalpado,Elizz M
Hernández-Cuéllar,Edgar E
Guerrero-Barrera,Alma L
Expression of Enamel Proteins in Human Dental Pulp Stem Cells by the Effect of extracellular Matrix
description SUMMARY: Mesenchymal stem cells are present in adult tissues such as the human dental pulp. They are pluripotent and can differentiate into various specialized cell types in vitro through appropriate stimuli. Ameloblasts produce human tooth enamel only during embryonic development before tooth eruption, so endogenous regeneration is not possible. Various efforts have been aimed at generating natural or artificial substitutes for dental enamel with properties similar to the specific components of said tissue. The purpose of this study was to induce human dental pulp stem cells to produce enamel proteins using extracellular matrix derived from the rat tail tendon and pigskin. Primary cultures of human dental pulp stem cells were established and characterized by RT-PCR and immunofluorescence, using mesenchymal cell markers such as CD14, CD40, CD44, CD105, and STRO-1. The cells were then incubated with the extracellular matrix for fourteen days and labeled with specific antibodies to detect the expression of dental enamel proteins such as amelogenin, ameloblastin, enamelisin, tuftelin, and parvalbumin, characteristics of the phenotype of ameloblasts. This work demonstrated a positive effect of the extracellular matrix to induce the expression of enamel proteins in the stem cells of the human dental pulp.
author Salazar-de-Santiago,Alfredo
Avelar-González,Francisco J
Díaz,Juan Manuel
Campos-Navarro,Paloma M
Flores-Villalpado,Elizz M
Hernández-Cuéllar,Edgar E
Guerrero-Barrera,Alma L
author_facet Salazar-de-Santiago,Alfredo
Avelar-González,Francisco J
Díaz,Juan Manuel
Campos-Navarro,Paloma M
Flores-Villalpado,Elizz M
Hernández-Cuéllar,Edgar E
Guerrero-Barrera,Alma L
author_sort Salazar-de-Santiago,Alfredo
title Expression of Enamel Proteins in Human Dental Pulp Stem Cells by the Effect of extracellular Matrix
title_short Expression of Enamel Proteins in Human Dental Pulp Stem Cells by the Effect of extracellular Matrix
title_full Expression of Enamel Proteins in Human Dental Pulp Stem Cells by the Effect of extracellular Matrix
title_fullStr Expression of Enamel Proteins in Human Dental Pulp Stem Cells by the Effect of extracellular Matrix
title_full_unstemmed Expression of Enamel Proteins in Human Dental Pulp Stem Cells by the Effect of extracellular Matrix
title_sort expression of enamel proteins in human dental pulp stem cells by the effect of extracellular matrix
publisher Sociedad Chilena de Anatomía
publishDate 2020
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601742
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