Ameliorative Effect of Resveratrol Against Histological Alterations in Lung of Mice Induced by Subchronic Exposure of Cigarette and Waterpipe Tobacco Smoking

SUMMARY: This study aims to evaluate the ability of resveratrol (RSV) antioxidant to attenuate the oxidative stress condition induced by secondhand exposure of cigarettes and waterpipe smoking using animal model. Forty-eight mice were divided equally into six different groups, and RSV was delivered...

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Autores principales:	Alzbeede,Ahmed A, Shraideh,Ziad A, Badran,Darwish H
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Antioxidant Microscopy Histopathology TEM Tobacco smoking
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100011
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spelling	oai:scielo:S0717-950220210001000112021-03-01Ameliorative Effect of Resveratrol Against Histological Alterations in Lung of Mice Induced by Subchronic Exposure of Cigarette and Waterpipe Tobacco SmokingAlzbeede,Ahmed AShraideh,Ziad ABadran,Darwish H Antioxidant Microscopy Histopathology TEM Tobacco smoking SUMMARY: This study aims to evaluate the ability of resveratrol (RSV) antioxidant to attenuate the oxidative stress condition induced by secondhand exposure of cigarettes and waterpipe smoking using animal model. Forty-eight mice were divided equally into six different groups, and RSV was delivered to certain groups intraperitoneally with a dose of 25 mg/kg/day. The process of smoking exposure was performed using a specialized smoking machine. The experiment duration lasts for six consecutive weeks. Five µm sections of lung were stained with hematoxylin and eosin for light microscopy, and 70 nm ultrathin sections of lung stained with uranyl acetate and lead citrate were prepared for transmission electron microscopy to observe the cellular ultrastructure. In lung, RSV reduced the deterioration and blebbing of bronchiole epithelium, reduced the inflammation, increased the surface area of alveolar sac, and reduced the thickening of alveolar walls. Also, blood vessels were less congested and less dilated with less diffusion of extravasated blood. Ultrastructural images illustrated that RSV protects the normal structure of alveolar septum, prevents DNA damage and alveolar degeneration, showed less degree of apical membrane blebbing and retained the uniform pattern of mitochondria. In conclusion, RSV has ameliorative effects against the oxidative stress condition induced by secondhand (side stream) exposure of cigarette and waterpipe tobacco smoking.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.1 20212021-02-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100011en10.4067/S0717-95022021000100011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antioxidant Microscopy Histopathology TEM Tobacco smoking
spellingShingle	Antioxidant Microscopy Histopathology TEM Tobacco smoking Alzbeede,Ahmed A Shraideh,Ziad A Badran,Darwish H Ameliorative Effect of Resveratrol Against Histological Alterations in Lung of Mice Induced by Subchronic Exposure of Cigarette and Waterpipe Tobacco Smoking
description	SUMMARY: This study aims to evaluate the ability of resveratrol (RSV) antioxidant to attenuate the oxidative stress condition induced by secondhand exposure of cigarettes and waterpipe smoking using animal model. Forty-eight mice were divided equally into six different groups, and RSV was delivered to certain groups intraperitoneally with a dose of 25 mg/kg/day. The process of smoking exposure was performed using a specialized smoking machine. The experiment duration lasts for six consecutive weeks. Five µm sections of lung were stained with hematoxylin and eosin for light microscopy, and 70 nm ultrathin sections of lung stained with uranyl acetate and lead citrate were prepared for transmission electron microscopy to observe the cellular ultrastructure. In lung, RSV reduced the deterioration and blebbing of bronchiole epithelium, reduced the inflammation, increased the surface area of alveolar sac, and reduced the thickening of alveolar walls. Also, blood vessels were less congested and less dilated with less diffusion of extravasated blood. Ultrastructural images illustrated that RSV protects the normal structure of alveolar septum, prevents DNA damage and alveolar degeneration, showed less degree of apical membrane blebbing and retained the uniform pattern of mitochondria. In conclusion, RSV has ameliorative effects against the oxidative stress condition induced by secondhand (side stream) exposure of cigarette and waterpipe tobacco smoking.
author	Alzbeede,Ahmed A Shraideh,Ziad A Badran,Darwish H
author_facet	Alzbeede,Ahmed A Shraideh,Ziad A Badran,Darwish H
author_sort	Alzbeede,Ahmed A
title	Ameliorative Effect of Resveratrol Against Histological Alterations in Lung of Mice Induced by Subchronic Exposure of Cigarette and Waterpipe Tobacco Smoking
title_short	Ameliorative Effect of Resveratrol Against Histological Alterations in Lung of Mice Induced by Subchronic Exposure of Cigarette and Waterpipe Tobacco Smoking
title_full	Ameliorative Effect of Resveratrol Against Histological Alterations in Lung of Mice Induced by Subchronic Exposure of Cigarette and Waterpipe Tobacco Smoking
title_fullStr	Ameliorative Effect of Resveratrol Against Histological Alterations in Lung of Mice Induced by Subchronic Exposure of Cigarette and Waterpipe Tobacco Smoking
title_full_unstemmed	Ameliorative Effect of Resveratrol Against Histological Alterations in Lung of Mice Induced by Subchronic Exposure of Cigarette and Waterpipe Tobacco Smoking
title_sort	ameliorative effect of resveratrol against histological alterations in lung of mice induced by subchronic exposure of cigarette and waterpipe tobacco smoking
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100011
work_keys_str_mv	AT alzbeedeahmeda ameliorativeeffectofresveratrolagainsthistologicalalterationsinlungofmiceinducedbysubchronicexposureofcigaretteandwaterpipetobaccosmoking AT shraidehziada ameliorativeeffectofresveratrolagainsthistologicalalterationsinlungofmiceinducedbysubchronicexposureofcigaretteandwaterpipetobaccosmoking AT badrandarwishh ameliorativeeffectofresveratrolagainsthistologicalalterationsinlungofmiceinducedbysubchronicexposureofcigaretteandwaterpipetobaccosmoking
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Ameliorative Effect of Resveratrol Against Histological Alterations in Lung of Mice Induced by Subchronic Exposure of Cigarette and Waterpipe Tobacco Smoking

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