Different Expressions of AQP1 and AQP4 in Hyponatremic Rat Brain

SUMMARY: The expression of aquaporin-1 (AQP1) in choroid plexus and aquaporin-4 (AQP4) in astrocyte of the hippocampal formation (HF) was studied in the rat to determine the role of AQP1 and AQP4 in the pathophysiology of systemic hyponatremia (SH). SH was induced by coadministration of dextrose sol...

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Autores principales:	Lim,Jun Hyeuck, Jung,Yong Wook, Park,Hyo Seok
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Aquaporin-1 Aquaporin-4 Systemic hyponatremia Cell death
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100064
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spelling	oai:scielo:S0717-950220210001000642021-03-01Different Expressions of AQP1 and AQP4 in Hyponatremic Rat BrainLim,Jun HyeuckJung,Yong WookPark,Hyo Seok Aquaporin-1 Aquaporin-4 Systemic hyponatremia Cell death SUMMARY: The expression of aquaporin-1 (AQP1) in choroid plexus and aquaporin-4 (AQP4) in astrocyte of the hippocampal formation (HF) was studied in the rat to determine the role of AQP1 and AQP4 in the pathophysiology of systemic hyponatremia (SH). SH was induced by coadministration of dextrose solution intraperitoneally and through subcutaneous implantation of an osmotic minipump containing 8-deamino-arginin vasopressin (50ng/µl/h) for 24 and 48 h. Twenty- four and 48 h after the drug administration, there were significant reductions in Na+ concentration (111 ± 5 and 104 ± 2 mmol) and serum osmolarity (240 ± 13 and 221 ± 14 mOsm/L) as compared with control values (140 ± 4.7 mmol and 296 ± 5.2 mOsm/L), (p<0.01). The expression of AQP1 in the choroid plexus was increased three to five times from 24 h to 48 h after SH (329.86 ± 10.2 % and 531.5 ± 4.4 %, n=4, p<0.01). In contrast, AQP4 expression was significantly decreased up to 48 h after SH (36 ± 9 %, n=4, p<0.01). Quantitative immunoblotting revealed significant decreases of neuronal proteins in the HF after 24 to 48 h of SH. Therefore, we suggest that altered expression of AQP1 and AQP4 plays important role in the pathogenesis of systemic hyponatremia.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.1 20212021-02-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100064en10.4067/S0717-95022021000100064
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aquaporin-1 Aquaporin-4 Systemic hyponatremia Cell death
spellingShingle	Aquaporin-1 Aquaporin-4 Systemic hyponatremia Cell death Lim,Jun Hyeuck Jung,Yong Wook Park,Hyo Seok Different Expressions of AQP1 and AQP4 in Hyponatremic Rat Brain
description	SUMMARY: The expression of aquaporin-1 (AQP1) in choroid plexus and aquaporin-4 (AQP4) in astrocyte of the hippocampal formation (HF) was studied in the rat to determine the role of AQP1 and AQP4 in the pathophysiology of systemic hyponatremia (SH). SH was induced by coadministration of dextrose solution intraperitoneally and through subcutaneous implantation of an osmotic minipump containing 8-deamino-arginin vasopressin (50ng/µl/h) for 24 and 48 h. Twenty- four and 48 h after the drug administration, there were significant reductions in Na+ concentration (111 ± 5 and 104 ± 2 mmol) and serum osmolarity (240 ± 13 and 221 ± 14 mOsm/L) as compared with control values (140 ± 4.7 mmol and 296 ± 5.2 mOsm/L), (p<0.01). The expression of AQP1 in the choroid plexus was increased three to five times from 24 h to 48 h after SH (329.86 ± 10.2 % and 531.5 ± 4.4 %, n=4, p<0.01). In contrast, AQP4 expression was significantly decreased up to 48 h after SH (36 ± 9 %, n=4, p<0.01). Quantitative immunoblotting revealed significant decreases of neuronal proteins in the HF after 24 to 48 h of SH. Therefore, we suggest that altered expression of AQP1 and AQP4 plays important role in the pathogenesis of systemic hyponatremia.
author	Lim,Jun Hyeuck Jung,Yong Wook Park,Hyo Seok
author_facet	Lim,Jun Hyeuck Jung,Yong Wook Park,Hyo Seok
author_sort	Lim,Jun Hyeuck
title	Different Expressions of AQP1 and AQP4 in Hyponatremic Rat Brain
title_short	Different Expressions of AQP1 and AQP4 in Hyponatremic Rat Brain
title_full	Different Expressions of AQP1 and AQP4 in Hyponatremic Rat Brain
title_fullStr	Different Expressions of AQP1 and AQP4 in Hyponatremic Rat Brain
title_full_unstemmed	Different Expressions of AQP1 and AQP4 in Hyponatremic Rat Brain
title_sort	different expressions of aqp1 and aqp4 in hyponatremic rat brain
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100064
work_keys_str_mv	AT limjunhyeuck differentexpressionsofaqp1andaqp4inhyponatremicratbrain AT jungyongwook differentexpressionsofaqp1andaqp4inhyponatremicratbrain AT parkhyoseok differentexpressionsofaqp1andaqp4inhyponatremicratbrain
_version_	1718445179906555904

Different Expressions of AQP1 and AQP4 in Hyponatremic Rat Brain

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