Prophylactic Effect of Potentilla fulgens on Renal Ischemia-Reperfusion Injury in Rats

SUMMARY: We aimed to investigate the possible protective effects of Potentilla fulgens on kidney tissue with ischemia- reperfusion using immunohistochemical methods. Wistar rats were grouped as sham, ischemia, ischemia-reperfusion (I/R) and I/R treated with Potentilla fulgens. Renal vessels of the l...

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Autores principales:	Yaris,Mehmet, Deveci,Engin
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Potentilla fulgens Renal ischemia, rat ADAMTS-5 Caspase-3
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100116
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spelling	oai:scielo:S0717-950220210001001162021-03-01Prophylactic Effect of Potentilla fulgens on Renal Ischemia-Reperfusion Injury in RatsYaris,MehmetDeveci,Engin Potentilla fulgens Renal ischemia, rat ADAMTS-5 Caspase-3 SUMMARY: We aimed to investigate the possible protective effects of Potentilla fulgens on kidney tissue with ischemia- reperfusion using immunohistochemical methods. Wistar rats were grouped as sham, ischemia, ischemia-reperfusion (I/R) and I/R treated with Potentilla fulgens. Renal vessels of the left rat kidney were clamped for 60 minutes for ischemia, IR group had 6 h of reperfusion. 400 mg/kg Potentilla fulgens were given intraperitoneally 5 days before ischemia+reperfusion procedure. Biochemical analysis (MDA, GSH and MPO) of samples were performed. Kidney tissues were fixed with 10 % neutral formalin and routine paraffin tissue follow-up protocol was applied, stained with routine Hematoxylin and Eosin. ADAMTS-5 and Caspase-3 immunostaining was applied for immunohistochemistry and examined under a light microscope. In the ischemia group, inflammation and congestion in the vessels and increased ADAMTS-5 expression in glomerular cells and tubule cells were observed. In reperfusion, an increase in degenerative glomerular cells, tubule cells and intertubular connective tissue and inflammatory cells ADAMTS-5 expression was observed. In the P. fulgens group, degeneration and inflammation decreased and positive ADAMTS-5 expression was observed. In the ischemia and ischemia reperfusion group, increased apoptotic appearance and Caspase-3 positive expression in glomerular and tubular cells, and negative expression in most cells in the P. fulgens group. Potentilla fulgens are thought to stop apoptotic cell development at a certain stage, which affects the cytokine mechanism and plays an important role in the reduction of inflammatory cells and angiogenic regulation.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.1 20212021-02-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100116en10.4067/S0717-95022021000100116
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Potentilla fulgens Renal ischemia, rat ADAMTS-5 Caspase-3
spellingShingle	Potentilla fulgens Renal ischemia, rat ADAMTS-5 Caspase-3 Yaris,Mehmet Deveci,Engin Prophylactic Effect of Potentilla fulgens on Renal Ischemia-Reperfusion Injury in Rats
description	SUMMARY: We aimed to investigate the possible protective effects of Potentilla fulgens on kidney tissue with ischemia- reperfusion using immunohistochemical methods. Wistar rats were grouped as sham, ischemia, ischemia-reperfusion (I/R) and I/R treated with Potentilla fulgens. Renal vessels of the left rat kidney were clamped for 60 minutes for ischemia, IR group had 6 h of reperfusion. 400 mg/kg Potentilla fulgens were given intraperitoneally 5 days before ischemia+reperfusion procedure. Biochemical analysis (MDA, GSH and MPO) of samples were performed. Kidney tissues were fixed with 10 % neutral formalin and routine paraffin tissue follow-up protocol was applied, stained with routine Hematoxylin and Eosin. ADAMTS-5 and Caspase-3 immunostaining was applied for immunohistochemistry and examined under a light microscope. In the ischemia group, inflammation and congestion in the vessels and increased ADAMTS-5 expression in glomerular cells and tubule cells were observed. In reperfusion, an increase in degenerative glomerular cells, tubule cells and intertubular connective tissue and inflammatory cells ADAMTS-5 expression was observed. In the P. fulgens group, degeneration and inflammation decreased and positive ADAMTS-5 expression was observed. In the ischemia and ischemia reperfusion group, increased apoptotic appearance and Caspase-3 positive expression in glomerular and tubular cells, and negative expression in most cells in the P. fulgens group. Potentilla fulgens are thought to stop apoptotic cell development at a certain stage, which affects the cytokine mechanism and plays an important role in the reduction of inflammatory cells and angiogenic regulation.
author	Yaris,Mehmet Deveci,Engin
author_facet	Yaris,Mehmet Deveci,Engin
author_sort	Yaris,Mehmet
title	Prophylactic Effect of Potentilla fulgens on Renal Ischemia-Reperfusion Injury in Rats
title_short	Prophylactic Effect of Potentilla fulgens on Renal Ischemia-Reperfusion Injury in Rats
title_full	Prophylactic Effect of Potentilla fulgens on Renal Ischemia-Reperfusion Injury in Rats
title_fullStr	Prophylactic Effect of Potentilla fulgens on Renal Ischemia-Reperfusion Injury in Rats
title_full_unstemmed	Prophylactic Effect of Potentilla fulgens on Renal Ischemia-Reperfusion Injury in Rats
title_sort	prophylactic effect of potentilla fulgens on renal ischemia-reperfusion injury in rats
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100116
work_keys_str_mv	AT yarismehmet prophylacticeffectofpotentillafulgensonrenalischemiareperfusioninjuryinrats AT deveciengin prophylacticeffectofpotentillafulgensonrenalischemiareperfusioninjuryinrats
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Prophylactic Effect of Potentilla fulgens on Renal Ischemia-Reperfusion Injury in Rats

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