A Comparative Immunohistochemical and Quantitative Study of the Epiligament of the Medial Collateral and Anterior Cruciate Ligament in Rat Knee

SUMMARY: The aim of the present study was to evaluate the importance of the epiligament for the difference in the healing potential of the knee anterior cruciate and medial collateral ligament. To do so, we compared the structure of the anterior cruciate and the medial collateral ligament and evalua...

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Autores principales:	Iliev,Alexandar, Kotov,Georgi, Stamenov,Nikola, Landzhov,Boycho, Kirkov,Vidin, Georgiev,Georgi P
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Anterior cruciate ligament Medial collateral ligament Epiligament Collagen Healing
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100151
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spelling	oai:scielo:S0717-950220210001001512021-03-01A Comparative Immunohistochemical and Quantitative Study of the Epiligament of the Medial Collateral and Anterior Cruciate Ligament in Rat KneeIliev,AlexandarKotov,GeorgiStamenov,NikolaLandzhov,BoychoKirkov,VidinGeorgiev,Georgi P Anterior cruciate ligament Medial collateral ligament Epiligament Collagen Healing SUMMARY: The aim of the present study was to evaluate the importance of the epiligament for the difference in the healing potential of the knee anterior cruciate and medial collateral ligament. To do so, we compared the structure of the anterior cruciate and the medial collateral ligament and evaluated the differences in the expression of collagen types I, III and V in a rat knee. We have also conducted a comparative quantitative analysis of the number of cells per mm2 in the two ligaments. Tissue samples were obtained from the anterior cruciate and medial collateral ligament of 10 knee joints taken from five 8-month-old Wistar rats. We used standard hematoxylin and eosin staining, in addition to immunohistochemical staining with monoclonal antibodies against collagen types I, III and V. A semi-quantitative analysis of the expression was made through ImageJ, while Student’s T-test was used for the statistical analysis. Our results showed higher expression of all collagen types in the epiligament, compared to the ligament proper and difference in the expression between the medial collateral and the anterior cruciate ligament in favor of the first. We also reported a statistically significant difference in the number of cells per mm2 between the two ligaments and their epiligaments. Our findings show a higher number of cells and a stronger expression of certain collagen types in the epiligament of the medial collateral compared to the anterior cruciate ligament, which may be related to the difference in their healing potential.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.1 20212021-02-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100151en10.4067/S0717-95022021000100151
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Anterior cruciate ligament Medial collateral ligament Epiligament Collagen Healing
spellingShingle	Anterior cruciate ligament Medial collateral ligament Epiligament Collagen Healing Iliev,Alexandar Kotov,Georgi Stamenov,Nikola Landzhov,Boycho Kirkov,Vidin Georgiev,Georgi P A Comparative Immunohistochemical and Quantitative Study of the Epiligament of the Medial Collateral and Anterior Cruciate Ligament in Rat Knee
description	SUMMARY: The aim of the present study was to evaluate the importance of the epiligament for the difference in the healing potential of the knee anterior cruciate and medial collateral ligament. To do so, we compared the structure of the anterior cruciate and the medial collateral ligament and evaluated the differences in the expression of collagen types I, III and V in a rat knee. We have also conducted a comparative quantitative analysis of the number of cells per mm2 in the two ligaments. Tissue samples were obtained from the anterior cruciate and medial collateral ligament of 10 knee joints taken from five 8-month-old Wistar rats. We used standard hematoxylin and eosin staining, in addition to immunohistochemical staining with monoclonal antibodies against collagen types I, III and V. A semi-quantitative analysis of the expression was made through ImageJ, while Student’s T-test was used for the statistical analysis. Our results showed higher expression of all collagen types in the epiligament, compared to the ligament proper and difference in the expression between the medial collateral and the anterior cruciate ligament in favor of the first. We also reported a statistically significant difference in the number of cells per mm2 between the two ligaments and their epiligaments. Our findings show a higher number of cells and a stronger expression of certain collagen types in the epiligament of the medial collateral compared to the anterior cruciate ligament, which may be related to the difference in their healing potential.
author	Iliev,Alexandar Kotov,Georgi Stamenov,Nikola Landzhov,Boycho Kirkov,Vidin Georgiev,Georgi P
author_facet	Iliev,Alexandar Kotov,Georgi Stamenov,Nikola Landzhov,Boycho Kirkov,Vidin Georgiev,Georgi P
author_sort	Iliev,Alexandar
title	A Comparative Immunohistochemical and Quantitative Study of the Epiligament of the Medial Collateral and Anterior Cruciate Ligament in Rat Knee
title_short	A Comparative Immunohistochemical and Quantitative Study of the Epiligament of the Medial Collateral and Anterior Cruciate Ligament in Rat Knee
title_full	A Comparative Immunohistochemical and Quantitative Study of the Epiligament of the Medial Collateral and Anterior Cruciate Ligament in Rat Knee
title_fullStr	A Comparative Immunohistochemical and Quantitative Study of the Epiligament of the Medial Collateral and Anterior Cruciate Ligament in Rat Knee
title_full_unstemmed	A Comparative Immunohistochemical and Quantitative Study of the Epiligament of the Medial Collateral and Anterior Cruciate Ligament in Rat Knee
title_sort	comparative immunohistochemical and quantitative study of the epiligament of the medial collateral and anterior cruciate ligament in rat knee
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100151
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A Comparative Immunohistochemical and Quantitative Study of the Epiligament of the Medial Collateral and Anterior Cruciate Ligament in Rat Knee

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