Ultrastructural Clarification of the Peripherally Located Actin Network in the Myelinated Axons

SUMMARY: Despite the existence of a large amount of actin in the axons, the concentration F-actin was quite low in the myelinated axons and almost all the F-actin were located in the peripheries of the myelinated axons. Until now, the ultrastructural localization of F-actin has still not been report...

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Autores principales:	Gui,Yue, Wu,Shuang, Tan,Bai-Hong, Xiong,Tian-Qing, Li,Yan-Chao
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Axon F-actin Electron microscopy Myelin sheath Rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100179
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id	oai:scielo:S0717-95022021000100179
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spelling	oai:scielo:S0717-950220210001001792021-03-01Ultrastructural Clarification of the Peripherally Located Actin Network in the Myelinated AxonsGui,YueWu,ShuangTan,Bai-HongXiong,Tian-QingLi,Yan-Chao Axon F-actin Electron microscopy Myelin sheath Rat SUMMARY: Despite the existence of a large amount of actin in the axons, the concentration F-actin was quite low in the myelinated axons and almost all the F-actin were located in the peripheries of the myelinated axons. Until now, the ultrastructural localization of F-actin has still not been reported in the myelinated axons, probably due to the lack of an appropriate detection method. In the present study, a phalloidin-based FITC-anti-FITC technique was adopted to investigate the subcellular localization of F-actin in the myelinated axons. By using this technique, F-actin is located in the outer and inner collars of myelinated cytoplasm surrounding the intermodal axon, the Schmidt-Lanterman incisures, the paranodal terminal loops and the nodal microvilli. In addition, the satellite cell envelope, which encapsulates the axonal initial segment of the peripheral sensory neuron, was also demonstrated as an F-actin-enriched structure. This study provided a hitherto unreported ultrastructural view of the F-actin in the myelinated axons, which may assist in understanding the unique organization of axonal actin cytoskeleton.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.1 20212021-02-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100179en10.4067/S0717-95022021000100179
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Axon F-actin Electron microscopy Myelin sheath Rat
spellingShingle	Axon F-actin Electron microscopy Myelin sheath Rat Gui,Yue Wu,Shuang Tan,Bai-Hong Xiong,Tian-Qing Li,Yan-Chao Ultrastructural Clarification of the Peripherally Located Actin Network in the Myelinated Axons
description	SUMMARY: Despite the existence of a large amount of actin in the axons, the concentration F-actin was quite low in the myelinated axons and almost all the F-actin were located in the peripheries of the myelinated axons. Until now, the ultrastructural localization of F-actin has still not been reported in the myelinated axons, probably due to the lack of an appropriate detection method. In the present study, a phalloidin-based FITC-anti-FITC technique was adopted to investigate the subcellular localization of F-actin in the myelinated axons. By using this technique, F-actin is located in the outer and inner collars of myelinated cytoplasm surrounding the intermodal axon, the Schmidt-Lanterman incisures, the paranodal terminal loops and the nodal microvilli. In addition, the satellite cell envelope, which encapsulates the axonal initial segment of the peripheral sensory neuron, was also demonstrated as an F-actin-enriched structure. This study provided a hitherto unreported ultrastructural view of the F-actin in the myelinated axons, which may assist in understanding the unique organization of axonal actin cytoskeleton.
author	Gui,Yue Wu,Shuang Tan,Bai-Hong Xiong,Tian-Qing Li,Yan-Chao
author_facet	Gui,Yue Wu,Shuang Tan,Bai-Hong Xiong,Tian-Qing Li,Yan-Chao
author_sort	Gui,Yue
title	Ultrastructural Clarification of the Peripherally Located Actin Network in the Myelinated Axons
title_short	Ultrastructural Clarification of the Peripherally Located Actin Network in the Myelinated Axons
title_full	Ultrastructural Clarification of the Peripherally Located Actin Network in the Myelinated Axons
title_fullStr	Ultrastructural Clarification of the Peripherally Located Actin Network in the Myelinated Axons
title_full_unstemmed	Ultrastructural Clarification of the Peripherally Located Actin Network in the Myelinated Axons
title_sort	ultrastructural clarification of the peripherally located actin network in the myelinated axons
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100179
work_keys_str_mv	AT guiyue ultrastructuralclarificationoftheperipherallylocatedactinnetworkinthemyelinatedaxons AT wushuang ultrastructuralclarificationoftheperipherallylocatedactinnetworkinthemyelinatedaxons AT tanbaihong ultrastructuralclarificationoftheperipherallylocatedactinnetworkinthemyelinatedaxons AT xiongtianqing ultrastructuralclarificationoftheperipherallylocatedactinnetworkinthemyelinatedaxons AT liyanchao ultrastructuralclarificationoftheperipherallylocatedactinnetworkinthemyelinatedaxons
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Ultrastructural Clarification of the Peripherally Located Actin Network in the Myelinated Axons

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