Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis

SUMMARY: The aim of the present study was to evaluate the effect of different staining techniques on applicability and accuracy of tooth cementum annulation (TCA) method. Nine decalcination techniques, 8 dehydration protocols and 8 different techniques were applied in 3 teeth from the persons of a k...

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Autores principales:	Petrovic,Bojan, Pantelinac,Jelena, Capo,Ivan, Miljkovic,Dejan, Popovic,Milan, Penezic,Kristina, Stefanovic,Sofija
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Dental cementum Increment lines Age estimation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100216
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spelling	oai:scielo:S0717-950220210001002162021-03-01Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation AnalysisPetrovic,BojanPantelinac,JelenaCapo,IvanMiljkovic,DejanPopovic,MilanPenezic,KristinaStefanovic,Sofija Dental cementum Increment lines Age estimation SUMMARY: The aim of the present study was to evaluate the effect of different staining techniques on applicability and accuracy of tooth cementum annulation (TCA) method. Nine decalcination techniques, 8 dehydration protocols and 8 different techniques were applied in 3 teeth from the persons of a known age. Black and white, and color images of histological sections were captured. An x- ray was taken of each tooth and they were photographed. Researchers were asked to observe both black/white and color images of histological sections. Researchers were divided into two groups. The first group analyzed histological images only, and the second group had photos of teeth and X-rays. In the first group of observers (without X ray) the differences in age estimation between real and observed age were significant for 2 younger patients, but not for the oldest patient, where the observed and real values matched. Of the 6 raters, the assesments of the last 3 (that used x-ray images together with histological sections) did not differ significantly from the real values. Extensive analysis and multiple repetitions performed in the present investigation revealed that the most optimal method of decalcification for TCA method was EDTA II for a period longer than 14 days at a section thickness of 2-3mm, while the most optimal protocol for dehydration was number IV. When it comes to staining, the most optimal staining protocol used for the cemental lines visualization and counting was Crocein Scarlet/Acid Fuchsin staining and Toluidine blue staining used at semithin section. Additional use of preexperimental evaluation employing x-ray of analyzed teeth decreased the errors of age estimation.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.1 20212021-02-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100216en10.4067/S0717-95022021000100216
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Dental cementum Increment lines Age estimation
spellingShingle	Dental cementum Increment lines Age estimation Petrovic,Bojan Pantelinac,Jelena Capo,Ivan Miljkovic,Dejan Popovic,Milan Penezic,Kristina Stefanovic,Sofija Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis
description	SUMMARY: The aim of the present study was to evaluate the effect of different staining techniques on applicability and accuracy of tooth cementum annulation (TCA) method. Nine decalcination techniques, 8 dehydration protocols and 8 different techniques were applied in 3 teeth from the persons of a known age. Black and white, and color images of histological sections were captured. An x- ray was taken of each tooth and they were photographed. Researchers were asked to observe both black/white and color images of histological sections. Researchers were divided into two groups. The first group analyzed histological images only, and the second group had photos of teeth and X-rays. In the first group of observers (without X ray) the differences in age estimation between real and observed age were significant for 2 younger patients, but not for the oldest patient, where the observed and real values matched. Of the 6 raters, the assesments of the last 3 (that used x-ray images together with histological sections) did not differ significantly from the real values. Extensive analysis and multiple repetitions performed in the present investigation revealed that the most optimal method of decalcification for TCA method was EDTA II for a period longer than 14 days at a section thickness of 2-3mm, while the most optimal protocol for dehydration was number IV. When it comes to staining, the most optimal staining protocol used for the cemental lines visualization and counting was Crocein Scarlet/Acid Fuchsin staining and Toluidine blue staining used at semithin section. Additional use of preexperimental evaluation employing x-ray of analyzed teeth decreased the errors of age estimation.
author	Petrovic,Bojan Pantelinac,Jelena Capo,Ivan Miljkovic,Dejan Popovic,Milan Penezic,Kristina Stefanovic,Sofija
author_facet	Petrovic,Bojan Pantelinac,Jelena Capo,Ivan Miljkovic,Dejan Popovic,Milan Penezic,Kristina Stefanovic,Sofija
author_sort	Petrovic,Bojan
title	Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis
title_short	Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis
title_full	Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis
title_fullStr	Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis
title_full_unstemmed	Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis
title_sort	using histological staining techniques to improve visualization and interpretability of tooth cementum annulation analysis
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000100216
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_version_	1718445184149094400

Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis

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