Immunohistochemical Distribution of Calcium Binding Proteins in the Cochlear Nuclear Complex of Circling Mice

SUMMARY: The term “circling mouse” refers to an animal model of deafness, in which the mouse exhibits circling, head tossing, and hyperactivity, with pathological features including degenerated spiral ganglion cells in the cochlea, and the loss of the organ of Corti. The cochlear...

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Autores principales:	Ryu,Ji Ho, Pradhan,Jonu, Kim,Myeung Ju, Maskey,Dhiraj
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Calretinin Calbindin D28-k Cochlear Nuclear Complex Circling mouse Parvalbumin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000200538
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spelling	oai:scielo:S0717-950220210002005382021-04-22Immunohistochemical Distribution of Calcium Binding Proteins in the Cochlear Nuclear Complex of Circling MiceRyu,Ji HoPradhan,JonuKim,Myeung JuMaskey,Dhiraj Calretinin Calbindin D28-k Cochlear Nuclear Complex Circling mouse Parvalbumin SUMMARY: The term “circling mouse” refers to an animal model of deafness, in which the mouse exhibits circling, head tossing, and hyperactivity, with pathological features including degenerated spiral ganglion cells in the cochlea, and the loss of the organ of Corti. The cochlear nuclear (CN) complex, a part of the auditory brain circuit, is essential to process both ascending and descending auditory information. Considering calcium’s (Ca2+) importance in homeostasis of numerous biological processes, hearing loss by cochlear damage, either by ablation or genetic defect, could cause changes in the Ca2+ concentration that might trigger functional and structural alterations in the auditory circuit. However, little is known about the correlation of the central nervous system (CNS) pathology in circling mice, especially of the auditory pathway circuit and Ca2+ changes. This present study investigates the distribution of Ca2+- binding proteins (CaBPs), calbindin D-28k (CB), parvalbumin (PV), and calretinin (CR) by using a free floating immunohistochemical method inthe CN of the wild-type mouse (+/+), the heterozygous mouse (+/cir), and the homozygous (cir/cir) mouse. CaBPs are well known to be an important factor that regulates Ca2+ concentrations. Compared with the dorsal and ventral cochlear nuclei of +/+ and +/ cirmice, prominent decreases of CaBPs’ immunoreactivity (IR) in cir/cirmice were observed in the somas, as well as in the neuropil. The present study reportson the overall distribution and changes in the immunoreactivity of CaBPs in the CN of cir/cirmice because ofa hearing defect. This data might be helpful to morphologically elucidate CNS disorders and their relation to CaBPs immunoreactivity related to hearing defects.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.2 20212021-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000200538en10.4067/S0717-95022021000200538
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Calretinin Calbindin D28-k Cochlear Nuclear Complex Circling mouse Parvalbumin
spellingShingle	Calretinin Calbindin D28-k Cochlear Nuclear Complex Circling mouse Parvalbumin Ryu,Ji Ho Pradhan,Jonu Kim,Myeung Ju Maskey,Dhiraj Immunohistochemical Distribution of Calcium Binding Proteins in the Cochlear Nuclear Complex of Circling Mice
description	SUMMARY: The term “circling mouse” refers to an animal model of deafness, in which the mouse exhibits circling, head tossing, and hyperactivity, with pathological features including degenerated spiral ganglion cells in the cochlea, and the loss of the organ of Corti. The cochlear nuclear (CN) complex, a part of the auditory brain circuit, is essential to process both ascending and descending auditory information. Considering calcium’s (Ca2+) importance in homeostasis of numerous biological processes, hearing loss by cochlear damage, either by ablation or genetic defect, could cause changes in the Ca2+ concentration that might trigger functional and structural alterations in the auditory circuit. However, little is known about the correlation of the central nervous system (CNS) pathology in circling mice, especially of the auditory pathway circuit and Ca2+ changes. This present study investigates the distribution of Ca2+- binding proteins (CaBPs), calbindin D-28k (CB), parvalbumin (PV), and calretinin (CR) by using a free floating immunohistochemical method inthe CN of the wild-type mouse (+/+), the heterozygous mouse (+/cir), and the homozygous (cir/cir) mouse. CaBPs are well known to be an important factor that regulates Ca2+ concentrations. Compared with the dorsal and ventral cochlear nuclei of +/+ and +/ cirmice, prominent decreases of CaBPs’ immunoreactivity (IR) in cir/cirmice were observed in the somas, as well as in the neuropil. The present study reportson the overall distribution and changes in the immunoreactivity of CaBPs in the CN of cir/cirmice because ofa hearing defect. This data might be helpful to morphologically elucidate CNS disorders and their relation to CaBPs immunoreactivity related to hearing defects.
author	Ryu,Ji Ho Pradhan,Jonu Kim,Myeung Ju Maskey,Dhiraj
author_facet	Ryu,Ji Ho Pradhan,Jonu Kim,Myeung Ju Maskey,Dhiraj
author_sort	Ryu,Ji Ho
title	Immunohistochemical Distribution of Calcium Binding Proteins in the Cochlear Nuclear Complex of Circling Mice
title_short	Immunohistochemical Distribution of Calcium Binding Proteins in the Cochlear Nuclear Complex of Circling Mice
title_full	Immunohistochemical Distribution of Calcium Binding Proteins in the Cochlear Nuclear Complex of Circling Mice
title_fullStr	Immunohistochemical Distribution of Calcium Binding Proteins in the Cochlear Nuclear Complex of Circling Mice
title_full_unstemmed	Immunohistochemical Distribution of Calcium Binding Proteins in the Cochlear Nuclear Complex of Circling Mice
title_sort	immunohistochemical distribution of calcium binding proteins in the cochlear nuclear complex of circling mice
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000200538
work_keys_str_mv	AT ryujiho immunohistochemicaldistributionofcalciumbindingproteinsinthecochlearnuclearcomplexofcirclingmice AT pradhanjonu immunohistochemicaldistributionofcalciumbindingproteinsinthecochlearnuclearcomplexofcirclingmice AT kimmyeungju immunohistochemicaldistributionofcalciumbindingproteinsinthecochlearnuclearcomplexofcirclingmice AT maskeydhiraj immunohistochemicaldistributionofcalciumbindingproteinsinthecochlearnuclearcomplexofcirclingmice
_version_	1718445192610054144

Immunohistochemical Distribution of Calcium Binding Proteins in the Cochlear Nuclear Complex of Circling Mice

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