Nuclear Elongation Correlates with Neurite Induced Cellular Elongation During Differentiation of SH-SY5Y Neural Cells

SUMMARY: Cellular differentiation is a highly regulated process that has vast implications for the mechanics of the cell. The interplay between differentiation induced cytoskeletal mechanical changes and strain on the nucleus is a potential cause of gene level changes. This study explores mechanical...

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Autor principal:	Balasubramanian,Priya S
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Cell Differentiation Nucleus Cytoskeleton Cell Mechanics Imaging
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000200548
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spelling	oai:scielo:S0717-950220210002005482021-04-22Nuclear Elongation Correlates with Neurite Induced Cellular Elongation During Differentiation of SH-SY5Y Neural CellsBalasubramanian,Priya S Cell Differentiation Nucleus Cytoskeleton Cell Mechanics Imaging SUMMARY: Cellular differentiation is a highly regulated process that has vast implications for the mechanics of the cell. The interplay between differentiation induced cytoskeletal mechanical changes and strain on the nucleus is a potential cause of gene level changes. This study explores mechanical changes in SH-SY5Y neural cells during differentiation mediated by Retinoic Acid (RA) across Days 0 through 9. Findings suggest that cellular elongation increases 1.92-fold over a 10-day differentiation period, from 48.97 ±16.85µm to 93.96 ± 31.20 µm over 3 repeated trials and across multiple cells analyzed on ImageJ. Nuclear elongation increases less substantially from 17.51 ± 2.71 µm to 23.26 ± 3.10 µm over 3 repeated trials and across multiple cells. Results are statistically significant at a significance level of α = 0.05. This study is one of the first studies to show that during the process of RA mediated neural differentiation in SH-SY5Y neural cells, nuclear elongation is initially not significantly correlated with cellular elongation, but it becomes correlated during the differentiation process with an overall correlation coefficient of 0.4498 at a significance level of α = 0.05. Given the time course of the mechanical changes and the known coupling between the cytoskeleton and nuclear lamina, this study suggests a causative and correlative relationship between neurite-driven cellular elongation and nuclear elongation during neural differentiation.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.2 20212021-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000200548en10.4067/S0717-95022021000200548
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cell Differentiation Nucleus Cytoskeleton Cell Mechanics Imaging
spellingShingle	Cell Differentiation Nucleus Cytoskeleton Cell Mechanics Imaging Balasubramanian,Priya S Nuclear Elongation Correlates with Neurite Induced Cellular Elongation During Differentiation of SH-SY5Y Neural Cells
description	SUMMARY: Cellular differentiation is a highly regulated process that has vast implications for the mechanics of the cell. The interplay between differentiation induced cytoskeletal mechanical changes and strain on the nucleus is a potential cause of gene level changes. This study explores mechanical changes in SH-SY5Y neural cells during differentiation mediated by Retinoic Acid (RA) across Days 0 through 9. Findings suggest that cellular elongation increases 1.92-fold over a 10-day differentiation period, from 48.97 ±16.85µm to 93.96 ± 31.20 µm over 3 repeated trials and across multiple cells analyzed on ImageJ. Nuclear elongation increases less substantially from 17.51 ± 2.71 µm to 23.26 ± 3.10 µm over 3 repeated trials and across multiple cells. Results are statistically significant at a significance level of α = 0.05. This study is one of the first studies to show that during the process of RA mediated neural differentiation in SH-SY5Y neural cells, nuclear elongation is initially not significantly correlated with cellular elongation, but it becomes correlated during the differentiation process with an overall correlation coefficient of 0.4498 at a significance level of α = 0.05. Given the time course of the mechanical changes and the known coupling between the cytoskeleton and nuclear lamina, this study suggests a causative and correlative relationship between neurite-driven cellular elongation and nuclear elongation during neural differentiation.
author	Balasubramanian,Priya S
author_facet	Balasubramanian,Priya S
author_sort	Balasubramanian,Priya S
title	Nuclear Elongation Correlates with Neurite Induced Cellular Elongation During Differentiation of SH-SY5Y Neural Cells
title_short	Nuclear Elongation Correlates with Neurite Induced Cellular Elongation During Differentiation of SH-SY5Y Neural Cells
title_full	Nuclear Elongation Correlates with Neurite Induced Cellular Elongation During Differentiation of SH-SY5Y Neural Cells
title_fullStr	Nuclear Elongation Correlates with Neurite Induced Cellular Elongation During Differentiation of SH-SY5Y Neural Cells
title_full_unstemmed	Nuclear Elongation Correlates with Neurite Induced Cellular Elongation During Differentiation of SH-SY5Y Neural Cells
title_sort	nuclear elongation correlates with neurite induced cellular elongation during differentiation of sh-sy5y neural cells
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000200548
work_keys_str_mv	AT balasubramanianpriyas nuclearelongationcorrelateswithneuriteinducedcellularelongationduringdifferentiationofshsy5yneuralcells
_version_	1718445192785166336

Nuclear Elongation Correlates with Neurite Induced Cellular Elongation During Differentiation of SH-SY5Y Neural Cells

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