Investigation of Effect of Paclitaxel on Netrin 1 and Factor 8 in Ehrlich Solid Tumors
SUMMARY: Cancer known as a malignant tumor, is a class of diseases involving abnormal cell growth with the potential to invade or spread to other parts of the body. The Ehrlich tumor is a mammary adenocarcinoma of mice developed in solid and ascitic forms. This study was aimed to investigate the eff...
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Sociedad Chilena de Anatomía
2021
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oai:scielo:S0717-950220210002005642021-04-22Investigation of Effect of Paclitaxel on Netrin 1 and Factor 8 in Ehrlich Solid TumorsKaymak,EminErtekin,TolgaÖzdamar,Saim Ehrlich Solid Tumor Paclitaxel Netrin 1 Factor 8 SUMMARY: Cancer known as a malignant tumor, is a class of diseases involving abnormal cell growth with the potential to invade or spread to other parts of the body. The Ehrlich tumor is a mammary adenocarcinoma of mice developed in solid and ascitic forms. This study was aimed to investigate the effects of paclitaxel on Netrin 1 and Factor 8 expression and also in tumor cell proliferation, apoptosis, angiogenesis, and development of tumor in Ehrlich solid tumors treated with paclitaxel. In this study, 26 adult Balb/C male mice were used. 6 of them were used as stock. Ehrlich ascites cells taken from animals in stock were injected subcutaneously from the neck area to all animals. The mice were randomly assigned to two groups of ten rats per group. Paclitaxel treatment group 10 mg/kg were administered to mice intraperitoneally (i.p.) 4,9, and 14th days. 15th day the animals were sacrificed and tumor tissues were taken. Paraffin-embedded solid tumor sections were stained Hematoxylin & Eosin, Masson’s Trichrome. Also solid tumor sections were stained immunohistochemically with Netrin1 and Factor 8. Tunel method was applied to determine apoptosis. Paclitaxel applied as a therapeutic Ehrlich solid tumor reduced the volume of tumors in the treatment groups. At the end of the experiments, in the treatment groups’ significantly reduced the Netrin 1 expression and microvessel density compared to the group control. Also paclitaxel in the treatment group increased the number of apoptotic cells. We suggest that decreasing the expression of Netrin 1 would be reduced vessel density and increased apoptosis.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.2 20212021-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000200564en10.4067/S0717-95022021000200564 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Ehrlich Solid Tumor Paclitaxel Netrin 1 Factor 8 |
| spellingShingle |
Ehrlich Solid Tumor Paclitaxel Netrin 1 Factor 8 Kaymak,Emin Ertekin,Tolga Özdamar,Saim Investigation of Effect of Paclitaxel on Netrin 1 and Factor 8 in Ehrlich Solid Tumors |
| description |
SUMMARY: Cancer known as a malignant tumor, is a class of diseases involving abnormal cell growth with the potential to invade or spread to other parts of the body. The Ehrlich tumor is a mammary adenocarcinoma of mice developed in solid and ascitic forms. This study was aimed to investigate the effects of paclitaxel on Netrin 1 and Factor 8 expression and also in tumor cell proliferation, apoptosis, angiogenesis, and development of tumor in Ehrlich solid tumors treated with paclitaxel. In this study, 26 adult Balb/C male mice were used. 6 of them were used as stock. Ehrlich ascites cells taken from animals in stock were injected subcutaneously from the neck area to all animals. The mice were randomly assigned to two groups of ten rats per group. Paclitaxel treatment group 10 mg/kg were administered to mice intraperitoneally (i.p.) 4,9, and 14th days. 15th day the animals were sacrificed and tumor tissues were taken. Paraffin-embedded solid tumor sections were stained Hematoxylin & Eosin, Masson’s Trichrome. Also solid tumor sections were stained immunohistochemically with Netrin1 and Factor 8. Tunel method was applied to determine apoptosis. Paclitaxel applied as a therapeutic Ehrlich solid tumor reduced the volume of tumors in the treatment groups. At the end of the experiments, in the treatment groups’ significantly reduced the Netrin 1 expression and microvessel density compared to the group control. Also paclitaxel in the treatment group increased the number of apoptotic cells. We suggest that decreasing the expression of Netrin 1 would be reduced vessel density and increased apoptosis. |
| author |
Kaymak,Emin Ertekin,Tolga Özdamar,Saim |
| author_facet |
Kaymak,Emin Ertekin,Tolga Özdamar,Saim |
| author_sort |
Kaymak,Emin |
| title |
Investigation of Effect of Paclitaxel on Netrin 1 and Factor 8 in Ehrlich Solid Tumors |
| title_short |
Investigation of Effect of Paclitaxel on Netrin 1 and Factor 8 in Ehrlich Solid Tumors |
| title_full |
Investigation of Effect of Paclitaxel on Netrin 1 and Factor 8 in Ehrlich Solid Tumors |
| title_fullStr |
Investigation of Effect of Paclitaxel on Netrin 1 and Factor 8 in Ehrlich Solid Tumors |
| title_full_unstemmed |
Investigation of Effect of Paclitaxel on Netrin 1 and Factor 8 in Ehrlich Solid Tumors |
| title_sort |
investigation of effect of paclitaxel on netrin 1 and factor 8 in ehrlich solid tumors |
| publisher |
Sociedad Chilena de Anatomía |
| publishDate |
2021 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000200564 |
| work_keys_str_mv |
AT kaymakemin investigationofeffectofpaclitaxelonnetrin1andfactor8inehrlichsolidtumors AT ertekintolga investigationofeffectofpaclitaxelonnetrin1andfactor8inehrlichsolidtumors AT ozdamarsaim investigationofeffectofpaclitaxelonnetrin1andfactor8inehrlichsolidtumors |
| _version_ |
1718445193356640256 |