Hepatic Mitochondria in Diet-induced Obese Mice (Stereology and Molecular Analysis)

SUMMARY: The world population is going through an obesity epidemic that has severe consequences for the health system. This study focused on studying hepatic mitochondria in obese animals induced by a high-fat (HF) diet and used the model-based stereology in electron micrographs for the quantitative...

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Autores principales:	Reis-Barbosa,Pedro Henrique, Carvalho,Jorge Jose de, del Sol,Mariano, Mandarim-de-Lacerda,Carlos Alberto
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Mitochondria Mitochondrial biogenesis Diet-induced obesity Cell biology Molecular biology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000200571
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spelling	oai:scielo:S0717-950220210002005712021-04-22Hepatic Mitochondria in Diet-induced Obese Mice (Stereology and Molecular Analysis)Reis-Barbosa,Pedro HenriqueCarvalho,Jorge Jose dedel Sol,MarianoMandarim-de-Lacerda,Carlos Alberto Mitochondria Mitochondrial biogenesis Diet-induced obesity Cell biology Molecular biology SUMMARY: The world population is going through an obesity epidemic that has severe consequences for the health system. This study focused on studying hepatic mitochondria in obese animals induced by a high-fat (HF) diet and used the model-based stereology in electron micrographs for the quantitative study. Besides, the gene expressions of molecular markers of mitochondrial biogenesis carnitine palmitoyltransferase 1a (Cpt 1α), mitochondrial transcription factor a (Tfam), uncoupling protein 3 (Ucp 3), and nuclear respiratory factor 1 (Nrf 1) were analyzed. The HF diet caused a weight gain of +1820 % comparing the control group (C) with the HF group (from 0.32±0.31 g to 5.5±0.39 g, P<0.001). The HF group showed fat droplets in the hepatocyte cytoplasm (steatosis) and less dense and large mitochondria in transmission electron microscopy. The mitochondria size (cross-section) did not show a significant difference between the groups C and HF. However, the mitochondria numerical density per area was 30 % less, the mitochondrial surface density (outer membrane) was 20 % less, and the mitochondrial volume density was 22 % less in the HF group than the C group. The gene expressions of molecular markers of mitochondrial biogenesis Cpt 1α, Tfam, Ucp 3, and Nrf 1 decreased in the HF group compared to the C group. The quantitative results match perfectly with the molecular ones of mitochondrial biogenesis markers. In the future, it will be crucial to verify if and how these data recover with the reduction of obesity, which would be of significant interest given the current obesity epidemic that affects the world population.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.2 20212021-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000200571en10.4067/S0717-95022021000200571
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Mitochondria Mitochondrial biogenesis Diet-induced obesity Cell biology Molecular biology
spellingShingle	Mitochondria Mitochondrial biogenesis Diet-induced obesity Cell biology Molecular biology Reis-Barbosa,Pedro Henrique Carvalho,Jorge Jose de del Sol,Mariano Mandarim-de-Lacerda,Carlos Alberto Hepatic Mitochondria in Diet-induced Obese Mice (Stereology and Molecular Analysis)
description	SUMMARY: The world population is going through an obesity epidemic that has severe consequences for the health system. This study focused on studying hepatic mitochondria in obese animals induced by a high-fat (HF) diet and used the model-based stereology in electron micrographs for the quantitative study. Besides, the gene expressions of molecular markers of mitochondrial biogenesis carnitine palmitoyltransferase 1a (Cpt 1α), mitochondrial transcription factor a (Tfam), uncoupling protein 3 (Ucp 3), and nuclear respiratory factor 1 (Nrf 1) were analyzed. The HF diet caused a weight gain of +1820 % comparing the control group (C) with the HF group (from 0.32±0.31 g to 5.5±0.39 g, P<0.001). The HF group showed fat droplets in the hepatocyte cytoplasm (steatosis) and less dense and large mitochondria in transmission electron microscopy. The mitochondria size (cross-section) did not show a significant difference between the groups C and HF. However, the mitochondria numerical density per area was 30 % less, the mitochondrial surface density (outer membrane) was 20 % less, and the mitochondrial volume density was 22 % less in the HF group than the C group. The gene expressions of molecular markers of mitochondrial biogenesis Cpt 1α, Tfam, Ucp 3, and Nrf 1 decreased in the HF group compared to the C group. The quantitative results match perfectly with the molecular ones of mitochondrial biogenesis markers. In the future, it will be crucial to verify if and how these data recover with the reduction of obesity, which would be of significant interest given the current obesity epidemic that affects the world population.
author	Reis-Barbosa,Pedro Henrique Carvalho,Jorge Jose de del Sol,Mariano Mandarim-de-Lacerda,Carlos Alberto
author_facet	Reis-Barbosa,Pedro Henrique Carvalho,Jorge Jose de del Sol,Mariano Mandarim-de-Lacerda,Carlos Alberto
author_sort	Reis-Barbosa,Pedro Henrique
title	Hepatic Mitochondria in Diet-induced Obese Mice (Stereology and Molecular Analysis)
title_short	Hepatic Mitochondria in Diet-induced Obese Mice (Stereology and Molecular Analysis)
title_full	Hepatic Mitochondria in Diet-induced Obese Mice (Stereology and Molecular Analysis)
title_fullStr	Hepatic Mitochondria in Diet-induced Obese Mice (Stereology and Molecular Analysis)
title_full_unstemmed	Hepatic Mitochondria in Diet-induced Obese Mice (Stereology and Molecular Analysis)
title_sort	hepatic mitochondria in diet-induced obese mice (stereology and molecular analysis)
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000200571
work_keys_str_mv	AT reisbarbosapedrohenrique hepaticmitochondriaindietinducedobesemicestereologyandmolecularanalysis AT carvalhojorgejosede hepaticmitochondriaindietinducedobesemicestereologyandmolecularanalysis AT delsolmariano hepaticmitochondriaindietinducedobesemicestereologyandmolecularanalysis AT mandarimdelacerdacarlosalberto hepaticmitochondriaindietinducedobesemicestereologyandmolecularanalysis
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Hepatic Mitochondria in Diet-induced Obese Mice (Stereology and Molecular Analysis)

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