Morphometric Study of Rat Sciatic Nerve Recovery after Three Nerve Repair Techniques: Epineural Suture, Polyethylene Glycol Hydrogel and Fibrin Sealant

SUMMARY: The effectiveness of microsurgical technique has a direct impact on the recovery of the injured peripheral nerve. The aim of our study was to investigate the result of sciatic nerve regeneration in rats after complete neurotomy and after nerve repair techniques including: 1) epineural sutur...

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Autores principales:	Goncharuk-Oleksii,O, Savosko-Serhii,I, Petriv-Taras,I, Medvediev-Volodymyr,V, Tsymbaliuk-Vitaly,I
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Sciatic nerve Regeneration Epineural suture Polyethylene glycol Fibrin glue
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000300677
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spelling	oai:scielo:S0717-950220210003006772021-07-25Morphometric Study of Rat Sciatic Nerve Recovery after Three Nerve Repair Techniques: Epineural Suture, Polyethylene Glycol Hydrogel and Fibrin SealantGoncharuk-Oleksii,OSavosko-Serhii,IPetriv-Taras,IMedvediev-Volodymyr,VTsymbaliuk-Vitaly,I Sciatic nerve Regeneration Epineural suture Polyethylene glycol Fibrin glue SUMMARY: The effectiveness of microsurgical technique has a direct impact on the recovery of the injured peripheral nerve. The aim of our study was to investigate the result of sciatic nerve regeneration in rats after complete neurotomy and after nerve repair techniques including: 1) epineural suture; 2) polyethylene glycol hydrogel (PEG) (DuraSeal); 3) fibrin sealant (Tisseel). The cross-section of distal sciatic nerve was studied at 14th, 30th and 60th days after nerve repair. Morphometry of myelinated nerve fibers in the distal stump of the sciatic nerve was performed. A significant increase in the number of myelinated nerve fibers was found, especially between 14 and 30 days. The density of myelinated nerve fibers in the distal stump at day 60 was significantly higher after using nerve repair technique including PEG and fibrin versus epineural suture (29.2 % and 32.1 % versus 21.5 %, P <0.05), and a higher level of remyelination of nerve fibers observed in the group with PEG. On day 60, complete elimination of PEG and fibrin sealant was not observed, encapsulation was found around the clusters of hydrogel. Thereby, three peripheral nerve repair techniques were equally effective, only with the use of PEG remyelination of nerve fibers was increasing.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.3 20212021-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000300677en10.4067/S0717-95022021000300677
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Sciatic nerve Regeneration Epineural suture Polyethylene glycol Fibrin glue
spellingShingle	Sciatic nerve Regeneration Epineural suture Polyethylene glycol Fibrin glue Goncharuk-Oleksii,O Savosko-Serhii,I Petriv-Taras,I Medvediev-Volodymyr,V Tsymbaliuk-Vitaly,I Morphometric Study of Rat Sciatic Nerve Recovery after Three Nerve Repair Techniques: Epineural Suture, Polyethylene Glycol Hydrogel and Fibrin Sealant
description	SUMMARY: The effectiveness of microsurgical technique has a direct impact on the recovery of the injured peripheral nerve. The aim of our study was to investigate the result of sciatic nerve regeneration in rats after complete neurotomy and after nerve repair techniques including: 1) epineural suture; 2) polyethylene glycol hydrogel (PEG) (DuraSeal); 3) fibrin sealant (Tisseel). The cross-section of distal sciatic nerve was studied at 14th, 30th and 60th days after nerve repair. Morphometry of myelinated nerve fibers in the distal stump of the sciatic nerve was performed. A significant increase in the number of myelinated nerve fibers was found, especially between 14 and 30 days. The density of myelinated nerve fibers in the distal stump at day 60 was significantly higher after using nerve repair technique including PEG and fibrin versus epineural suture (29.2 % and 32.1 % versus 21.5 %, P <0.05), and a higher level of remyelination of nerve fibers observed in the group with PEG. On day 60, complete elimination of PEG and fibrin sealant was not observed, encapsulation was found around the clusters of hydrogel. Thereby, three peripheral nerve repair techniques were equally effective, only with the use of PEG remyelination of nerve fibers was increasing.
author	Goncharuk-Oleksii,O Savosko-Serhii,I Petriv-Taras,I Medvediev-Volodymyr,V Tsymbaliuk-Vitaly,I
author_facet	Goncharuk-Oleksii,O Savosko-Serhii,I Petriv-Taras,I Medvediev-Volodymyr,V Tsymbaliuk-Vitaly,I
author_sort	Goncharuk-Oleksii,O
title	Morphometric Study of Rat Sciatic Nerve Recovery after Three Nerve Repair Techniques: Epineural Suture, Polyethylene Glycol Hydrogel and Fibrin Sealant
title_short	Morphometric Study of Rat Sciatic Nerve Recovery after Three Nerve Repair Techniques: Epineural Suture, Polyethylene Glycol Hydrogel and Fibrin Sealant
title_full	Morphometric Study of Rat Sciatic Nerve Recovery after Three Nerve Repair Techniques: Epineural Suture, Polyethylene Glycol Hydrogel and Fibrin Sealant
title_fullStr	Morphometric Study of Rat Sciatic Nerve Recovery after Three Nerve Repair Techniques: Epineural Suture, Polyethylene Glycol Hydrogel and Fibrin Sealant
title_full_unstemmed	Morphometric Study of Rat Sciatic Nerve Recovery after Three Nerve Repair Techniques: Epineural Suture, Polyethylene Glycol Hydrogel and Fibrin Sealant
title_sort	morphometric study of rat sciatic nerve recovery after three nerve repair techniques: epineural suture, polyethylene glycol hydrogel and fibrin sealant
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000300677
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Morphometric Study of Rat Sciatic Nerve Recovery after Three Nerve Repair Techniques: Epineural Suture, Polyethylene Glycol Hydrogel and Fibrin Sealant

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