Quercetin Inhibits ROS-p53-Bax-caspase-3 Axis of Apoptosis and Augments Gonadotropin and Testicular Hormones in Chronic Unpredictable Stress-Induced Testis Injury

SUMMARY: A large body of evidence supports the protective role of the flavonol antioxidant compound quercetin in mammals. We tested the hypothesis that quercetin can protect against the hypothalamus-pituitary-gonadal (HPG) axis defect like a reduction in gonadotropins and testicular hormones and abn...

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Autor principal:	Bin-Jaliah,Ismaeel
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Chronic stress HPG Axis Apoptosis Testicles Quercetin Rat model
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000300839
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spelling	oai:scielo:S0717-950220210003008392021-07-25Quercetin Inhibits ROS-p53-Bax-caspase-3 Axis of Apoptosis and Augments Gonadotropin and Testicular Hormones in Chronic Unpredictable Stress-Induced Testis InjuryBin-Jaliah,Ismaeel Chronic stress HPG Axis Apoptosis Testicles Quercetin Rat model SUMMARY: A large body of evidence supports the protective role of the flavonol antioxidant compound quercetin in mammals. We tested the hypothesis that quercetin can protect against the hypothalamus-pituitary-gonadal (HPG) axis defect like a reduction in gonadotropins and testicular hormones and abnormal semen analysis induced by chronic unpredictable stress (CUS), possibly via the downregulation of oxidative stress (ROS) and p53-Bax-caspase-3 pathways. Rats were either exposed to a variety of unpredictable stressors daily before being sacrificed after 3 weeks (model group) or were treated with quercetin (50 mg/kg body weight/day) at the same time the CUS were induced (treated group). Harvested testicular tissues were stained with basic histological staining, and testis homogenates were assayed for the tumor suppressor p53, apoptosis regulator Bax, B-cell lymphoma 2 (Bcl-2), caspase-3, malondialdehyde (MDA), glutathione peroxidase (GPx), and superoxide dismutase (SOD). In addition, harvested epididymis tissues were used to assess semen analysis, and blood samples were assayed for the testicular hormone testosterone, the adrenal cortex hormone corticosterone, and the anterior pituitary gonadotropins, follicular stimulating hormone (FSH) and luteinizing hormone (LH). CUS induced profound testicular damage and significantly (p<0.05) induced p53, Bax, caspase-3, MDA, and corticosterone, which were significantly (p<0.05) inhibited by quercetin except corticosterone. Whereas, quercetin significantly (p<0.05) increased FSH, LH, testosterone, Bcl-2, GPx, and SOD levels that were inhibited by CUS. In addition, CUS induced oligozoospermia, asthenozoospermia, and teratozoospermia, which were significantly (p<0.05) protected by quercetin. Thus, Quercetin protects against CUS-induced HPG defects in rats, which is associated with the inhibition of ROS-p53-Bax-caspase-3 axis.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.3 20212021-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000300839en10.4067/S0717-95022021000300839
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Chronic stress HPG Axis Apoptosis Testicles Quercetin Rat model
spellingShingle	Chronic stress HPG Axis Apoptosis Testicles Quercetin Rat model Bin-Jaliah,Ismaeel Quercetin Inhibits ROS-p53-Bax-caspase-3 Axis of Apoptosis and Augments Gonadotropin and Testicular Hormones in Chronic Unpredictable Stress-Induced Testis Injury
description	SUMMARY: A large body of evidence supports the protective role of the flavonol antioxidant compound quercetin in mammals. We tested the hypothesis that quercetin can protect against the hypothalamus-pituitary-gonadal (HPG) axis defect like a reduction in gonadotropins and testicular hormones and abnormal semen analysis induced by chronic unpredictable stress (CUS), possibly via the downregulation of oxidative stress (ROS) and p53-Bax-caspase-3 pathways. Rats were either exposed to a variety of unpredictable stressors daily before being sacrificed after 3 weeks (model group) or were treated with quercetin (50 mg/kg body weight/day) at the same time the CUS were induced (treated group). Harvested testicular tissues were stained with basic histological staining, and testis homogenates were assayed for the tumor suppressor p53, apoptosis regulator Bax, B-cell lymphoma 2 (Bcl-2), caspase-3, malondialdehyde (MDA), glutathione peroxidase (GPx), and superoxide dismutase (SOD). In addition, harvested epididymis tissues were used to assess semen analysis, and blood samples were assayed for the testicular hormone testosterone, the adrenal cortex hormone corticosterone, and the anterior pituitary gonadotropins, follicular stimulating hormone (FSH) and luteinizing hormone (LH). CUS induced profound testicular damage and significantly (p<0.05) induced p53, Bax, caspase-3, MDA, and corticosterone, which were significantly (p<0.05) inhibited by quercetin except corticosterone. Whereas, quercetin significantly (p<0.05) increased FSH, LH, testosterone, Bcl-2, GPx, and SOD levels that were inhibited by CUS. In addition, CUS induced oligozoospermia, asthenozoospermia, and teratozoospermia, which were significantly (p<0.05) protected by quercetin. Thus, Quercetin protects against CUS-induced HPG defects in rats, which is associated with the inhibition of ROS-p53-Bax-caspase-3 axis.
author	Bin-Jaliah,Ismaeel
author_facet	Bin-Jaliah,Ismaeel
author_sort	Bin-Jaliah,Ismaeel
title	Quercetin Inhibits ROS-p53-Bax-caspase-3 Axis of Apoptosis and Augments Gonadotropin and Testicular Hormones in Chronic Unpredictable Stress-Induced Testis Injury
title_short	Quercetin Inhibits ROS-p53-Bax-caspase-3 Axis of Apoptosis and Augments Gonadotropin and Testicular Hormones in Chronic Unpredictable Stress-Induced Testis Injury
title_full	Quercetin Inhibits ROS-p53-Bax-caspase-3 Axis of Apoptosis and Augments Gonadotropin and Testicular Hormones in Chronic Unpredictable Stress-Induced Testis Injury
title_fullStr	Quercetin Inhibits ROS-p53-Bax-caspase-3 Axis of Apoptosis and Augments Gonadotropin and Testicular Hormones in Chronic Unpredictable Stress-Induced Testis Injury
title_full_unstemmed	Quercetin Inhibits ROS-p53-Bax-caspase-3 Axis of Apoptosis and Augments Gonadotropin and Testicular Hormones in Chronic Unpredictable Stress-Induced Testis Injury
title_sort	quercetin inhibits ros-p53-bax-caspase-3 axis of apoptosis and augments gonadotropin and testicular hormones in chronic unpredictable stress-induced testis injury
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000300839
work_keys_str_mv	AT binjaliahismaeel quercetininhibitsrosp53baxcaspase3axisofapoptosisandaugmentsgonadotropinandtesticularhormonesinchronicunpredictablestressinducedtestisinjury
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Quercetin Inhibits ROS-p53-Bax-caspase-3 Axis of Apoptosis and Augments Gonadotropin and Testicular Hormones in Chronic Unpredictable Stress-Induced Testis Injury

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