Effects of Vitamin E on the Epithelial-Mesenchymal Transition in Testicular Development Exposed to Valproic Acid

SUMMARY: In testicular differentiation, somatic cells must adopt a specific destiny towards sustentacular, peritubular and interstitial cells, being fundamental for the morphogenesis of seminiferous tubules, mediated by morphogens such as Desert Hedgehog (DHH), insulin-like growth factor-1 (IGF-1) a...

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Autores principales:	Conei,Daniel, Rojas,Mariana, Santamaría,Luis, Risopatrón,Jennie
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Epithelial-mesenchymal transition Desert Hedgehog Insulin-like Growth Factor-1 Fibroblastic Growth Factor 2 Valproic acid
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000400947
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spelling	oai:scielo:S0717-950220210004009472021-08-16Effects of Vitamin E on the Epithelial-Mesenchymal Transition in Testicular Development Exposed to Valproic AcidConei,DanielRojas,MarianaSantamaría,LuisRisopatrón,Jennie Epithelial-mesenchymal transition Desert Hedgehog Insulin-like Growth Factor-1 Fibroblastic Growth Factor 2 Valproic acid SUMMARY: In testicular differentiation, somatic cells must adopt a specific destiny towards sustentacular, peritubular and interstitial cells, being fundamental for the morphogenesis of seminiferous tubules, mediated by morphogens such as Desert Hedgehog (DHH), insulin-like growth factor-1 (IGF-1) and fibroblastic growth factor 2 (FGF-2). Its alteration could be related to failures in the development mechanisms, such as those caused by valproic acid (VPA), which can be reversed with vitamin E (VE). The objective of the study was to evaluate the epithelial-mesenchymal transition (EMT) in the testicular development of mice exposed to VPA and VE. 12 groups of pregnant female mice were formed that were separated by days post-coital (dpc) at 12.5 dpc, 17.5 dpc and 6 weeks postnatal, each one subdivided into 4 groups of 5 pregnant women each. Subgroups received different treatments from the beginning to the end of gestation orally: 600 mg/kg of VPA, 600 mg/kg of VPA and 200 IU of VE, 200 IU of VE and the control group 0.3 mL of 0.9% physiological solution. Immunohistochemistry was performed for the detection of DHH, IGF-1 and FGF-2. Immunolocalization of DHH was observed in all stages, with more evident significant differences in integrated optical density (IOD) and percentage of immunoreaction area at 6 weeks postnatal, being lower in the VPA group. In IGF-1, lower intensity and distribution of immunostaining was observed in the fetal and pubertal stages in the VPA groups, a similar situation with FGF-2, but only evident at 17.5 dpc, with significant differences. These results demonstrate that VPA can alter EMT between somatic cells in testicular development, with VE being an agent capable of attenuating this process.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.4 20212021-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000400947en10.4067/S0717-95022021000400947
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Epithelial-mesenchymal transition Desert Hedgehog Insulin-like Growth Factor-1 Fibroblastic Growth Factor 2 Valproic acid
spellingShingle	Epithelial-mesenchymal transition Desert Hedgehog Insulin-like Growth Factor-1 Fibroblastic Growth Factor 2 Valproic acid Conei,Daniel Rojas,Mariana Santamaría,Luis Risopatrón,Jennie Effects of Vitamin E on the Epithelial-Mesenchymal Transition in Testicular Development Exposed to Valproic Acid
description	SUMMARY: In testicular differentiation, somatic cells must adopt a specific destiny towards sustentacular, peritubular and interstitial cells, being fundamental for the morphogenesis of seminiferous tubules, mediated by morphogens such as Desert Hedgehog (DHH), insulin-like growth factor-1 (IGF-1) and fibroblastic growth factor 2 (FGF-2). Its alteration could be related to failures in the development mechanisms, such as those caused by valproic acid (VPA), which can be reversed with vitamin E (VE). The objective of the study was to evaluate the epithelial-mesenchymal transition (EMT) in the testicular development of mice exposed to VPA and VE. 12 groups of pregnant female mice were formed that were separated by days post-coital (dpc) at 12.5 dpc, 17.5 dpc and 6 weeks postnatal, each one subdivided into 4 groups of 5 pregnant women each. Subgroups received different treatments from the beginning to the end of gestation orally: 600 mg/kg of VPA, 600 mg/kg of VPA and 200 IU of VE, 200 IU of VE and the control group 0.3 mL of 0.9% physiological solution. Immunohistochemistry was performed for the detection of DHH, IGF-1 and FGF-2. Immunolocalization of DHH was observed in all stages, with more evident significant differences in integrated optical density (IOD) and percentage of immunoreaction area at 6 weeks postnatal, being lower in the VPA group. In IGF-1, lower intensity and distribution of immunostaining was observed in the fetal and pubertal stages in the VPA groups, a similar situation with FGF-2, but only evident at 17.5 dpc, with significant differences. These results demonstrate that VPA can alter EMT between somatic cells in testicular development, with VE being an agent capable of attenuating this process.
author	Conei,Daniel Rojas,Mariana Santamaría,Luis Risopatrón,Jennie
author_facet	Conei,Daniel Rojas,Mariana Santamaría,Luis Risopatrón,Jennie
author_sort	Conei,Daniel
title	Effects of Vitamin E on the Epithelial-Mesenchymal Transition in Testicular Development Exposed to Valproic Acid
title_short	Effects of Vitamin E on the Epithelial-Mesenchymal Transition in Testicular Development Exposed to Valproic Acid
title_full	Effects of Vitamin E on the Epithelial-Mesenchymal Transition in Testicular Development Exposed to Valproic Acid
title_fullStr	Effects of Vitamin E on the Epithelial-Mesenchymal Transition in Testicular Development Exposed to Valproic Acid
title_full_unstemmed	Effects of Vitamin E on the Epithelial-Mesenchymal Transition in Testicular Development Exposed to Valproic Acid
title_sort	effects of vitamin e on the epithelial-mesenchymal transition in testicular development exposed to valproic acid
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000400947
work_keys_str_mv	AT coneidaniel effectsofvitamineontheepithelialmesenchymaltransitionintesticulardevelopmentexposedtovalproicacid AT rojasmariana effectsofvitamineontheepithelialmesenchymaltransitionintesticulardevelopmentexposedtovalproicacid AT santamarialuis effectsofvitamineontheepithelialmesenchymaltransitionintesticulardevelopmentexposedtovalproicacid AT risopatronjennie effectsofvitamineontheepithelialmesenchymaltransitionintesticulardevelopmentexposedtovalproicacid
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Effects of Vitamin E on the Epithelial-Mesenchymal Transition in Testicular Development Exposed to Valproic Acid

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