Characterization of SH-SY5Y Neurons Subject to 92kHz Ultrasound Stimulation

SUMMARY: Cellular microstructural changes due to ultrasound exposure are critical to understand and characterize in order to further the establishment of ultrasonics in cell and tissue engineering and medicine. In this study, neurite length, nuclear morphology, and cellular toxicity are assessed at...

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Autor principal:	Balasubramanian,Priya S
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Ultrasound Cell Biology in vitro Neural Stimulation Neural Differentiation Ultrasound Transducers
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000401109
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spelling	oai:scielo:S0717-950220210004011092021-08-16Characterization of SH-SY5Y Neurons Subject to 92kHz Ultrasound StimulationBalasubramanian,Priya S Ultrasound Cell Biology in vitro Neural Stimulation Neural Differentiation Ultrasound Transducers SUMMARY: Cellular microstructural changes due to ultrasound exposure are critical to understand and characterize in order to further the establishment of ultrasonics in cell and tissue engineering and medicine. In this study, neurite length, nuclear morphology, and cellular toxicity are assessed at varying intensities of 92 kHz ultrasound provided by a piezoceramic disk element and incident upon SH- SY5Y neurons in vitro. Findings suggest that stimulation increases neurite length up to 2.73 fold tested at α = 0.05 in an intensity dependent manner. Additionally, stimulation causes a statistically significant (α = 0.05) decrease in nuclear area and less elongated nuclei, by 1.78 fold and 1.38 fold respectively, also in an intensity dependent manner. For maximum transducer surface intensities ranging from 0 to 39.11 W/cm2, the toxicity of 92 kHz ultrasound is assessed and a nontoxic range is determined using Caspase-3 and Annexin V staining, in addition to Calcium imaging via Calcein-AM staining. Intensities of up to 1.6 W/cm2 are found to be nontoxic for the cells under the parameters used in this study.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.4 20212021-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000401109en10.4067/S0717-95022021000401109
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Ultrasound Cell Biology in vitro Neural Stimulation Neural Differentiation Ultrasound Transducers
spellingShingle	Ultrasound Cell Biology in vitro Neural Stimulation Neural Differentiation Ultrasound Transducers Balasubramanian,Priya S Characterization of SH-SY5Y Neurons Subject to 92kHz Ultrasound Stimulation
description	SUMMARY: Cellular microstructural changes due to ultrasound exposure are critical to understand and characterize in order to further the establishment of ultrasonics in cell and tissue engineering and medicine. In this study, neurite length, nuclear morphology, and cellular toxicity are assessed at varying intensities of 92 kHz ultrasound provided by a piezoceramic disk element and incident upon SH- SY5Y neurons in vitro. Findings suggest that stimulation increases neurite length up to 2.73 fold tested at α = 0.05 in an intensity dependent manner. Additionally, stimulation causes a statistically significant (α = 0.05) decrease in nuclear area and less elongated nuclei, by 1.78 fold and 1.38 fold respectively, also in an intensity dependent manner. For maximum transducer surface intensities ranging from 0 to 39.11 W/cm2, the toxicity of 92 kHz ultrasound is assessed and a nontoxic range is determined using Caspase-3 and Annexin V staining, in addition to Calcium imaging via Calcein-AM staining. Intensities of up to 1.6 W/cm2 are found to be nontoxic for the cells under the parameters used in this study.
author	Balasubramanian,Priya S
author_facet	Balasubramanian,Priya S
author_sort	Balasubramanian,Priya S
title	Characterization of SH-SY5Y Neurons Subject to 92kHz Ultrasound Stimulation
title_short	Characterization of SH-SY5Y Neurons Subject to 92kHz Ultrasound Stimulation
title_full	Characterization of SH-SY5Y Neurons Subject to 92kHz Ultrasound Stimulation
title_fullStr	Characterization of SH-SY5Y Neurons Subject to 92kHz Ultrasound Stimulation
title_full_unstemmed	Characterization of SH-SY5Y Neurons Subject to 92kHz Ultrasound Stimulation
title_sort	characterization of sh-sy5y neurons subject to 92khz ultrasound stimulation
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000401109
work_keys_str_mv	AT balasubramanianpriyas characterizationofshsy5yneuronssubjectto92khzultrasoundstimulation
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Characterization of SH-SY5Y Neurons Subject to 92kHz Ultrasound Stimulation

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