Estrogen Receptor mRNA Determined by Quantitative Real-Time PCR in the Nasal Mucosa of Women Taking Oral Contraceptives

SUMMARY: Estrogen receptors (ER) have been identified in human nasal mucosa, but its physiologic and pathologic impacts are not totally established. ER have been demonstrated in nasal mucosa by several authors, mainly by immunohistochemical method in nasal mucosa samples surgically removed. The pres...

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Autores principales:	Millas,Ieda, Richeti,Flávio, Correa,Carla Sant'Anna, Barros,Mirna Duarte, Jorge,Rodrigo Nishihara, Longui,Carlos Alberto
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Estrogen receptors Rhinitis Nasal mucosa RT-PCR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000401147
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spelling	oai:scielo:S0717-950220210004011472021-08-16Estrogen Receptor mRNA Determined by Quantitative Real-Time PCR in the Nasal Mucosa of Women Taking Oral ContraceptivesMillas,IedaRicheti,FlávioCorrea,Carla Sant'AnnaBarros,Mirna DuarteJorge,Rodrigo NishiharaLongui,Carlos Alberto Estrogen receptors Rhinitis Nasal mucosa RT-PCR SUMMARY: Estrogen receptors (ER) have been identified in human nasal mucosa, but its physiologic and pathologic impacts are not totally established. ER have been demonstrated in nasal mucosa by several authors, mainly by immunohistochemical method in nasal mucosa samples surgically removed. The present study aimed to quantify ERα and ERβ mRNA concentration by using an absolute quantitative real-time PCR in cells from nasal mucosa smear of women under oral contraceptive therapy. Nasal epithelium smear samples were collected from 110 patients divided in two groups: 55 women who present regular menstrual cycle without using contraceptives and 55 women who present regular menstrual cycle and have been using oral contraceptives for more than 3 months. All the patients answered a rhinitis symptoms questionnaire. The current study showed the potential usefulness of nasal turbinate mucosa cell sourcing, collected through swab, for extracting useful RNA for gene expression. We have identified the predominant expression of ERα isoform in a ratio 10-15 times higher compared to ERβ isoform. There is a tendency for positive correlation between the ERb isoform and the rhinitis severity score.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.4 20212021-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000401147en10.4067/S0717-95022021000401147
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Estrogen receptors Rhinitis Nasal mucosa RT-PCR
spellingShingle	Estrogen receptors Rhinitis Nasal mucosa RT-PCR Millas,Ieda Richeti,Flávio Correa,Carla Sant'Anna Barros,Mirna Duarte Jorge,Rodrigo Nishihara Longui,Carlos Alberto Estrogen Receptor mRNA Determined by Quantitative Real-Time PCR in the Nasal Mucosa of Women Taking Oral Contraceptives
description	SUMMARY: Estrogen receptors (ER) have been identified in human nasal mucosa, but its physiologic and pathologic impacts are not totally established. ER have been demonstrated in nasal mucosa by several authors, mainly by immunohistochemical method in nasal mucosa samples surgically removed. The present study aimed to quantify ERα and ERβ mRNA concentration by using an absolute quantitative real-time PCR in cells from nasal mucosa smear of women under oral contraceptive therapy. Nasal epithelium smear samples were collected from 110 patients divided in two groups: 55 women who present regular menstrual cycle without using contraceptives and 55 women who present regular menstrual cycle and have been using oral contraceptives for more than 3 months. All the patients answered a rhinitis symptoms questionnaire. The current study showed the potential usefulness of nasal turbinate mucosa cell sourcing, collected through swab, for extracting useful RNA for gene expression. We have identified the predominant expression of ERα isoform in a ratio 10-15 times higher compared to ERβ isoform. There is a tendency for positive correlation between the ERb isoform and the rhinitis severity score.
author	Millas,Ieda Richeti,Flávio Correa,Carla Sant'Anna Barros,Mirna Duarte Jorge,Rodrigo Nishihara Longui,Carlos Alberto
author_facet	Millas,Ieda Richeti,Flávio Correa,Carla Sant'Anna Barros,Mirna Duarte Jorge,Rodrigo Nishihara Longui,Carlos Alberto
author_sort	Millas,Ieda
title	Estrogen Receptor mRNA Determined by Quantitative Real-Time PCR in the Nasal Mucosa of Women Taking Oral Contraceptives
title_short	Estrogen Receptor mRNA Determined by Quantitative Real-Time PCR in the Nasal Mucosa of Women Taking Oral Contraceptives
title_full	Estrogen Receptor mRNA Determined by Quantitative Real-Time PCR in the Nasal Mucosa of Women Taking Oral Contraceptives
title_fullStr	Estrogen Receptor mRNA Determined by Quantitative Real-Time PCR in the Nasal Mucosa of Women Taking Oral Contraceptives
title_full_unstemmed	Estrogen Receptor mRNA Determined by Quantitative Real-Time PCR in the Nasal Mucosa of Women Taking Oral Contraceptives
title_sort	estrogen receptor mrna determined by quantitative real-time pcr in the nasal mucosa of women taking oral contraceptives
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000401147
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Estrogen Receptor mRNA Determined by Quantitative Real-Time PCR in the Nasal Mucosa of Women Taking Oral Contraceptives

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