Gonadal Differentiation During Embryonic and Fetal Development of Male New Zealand Rabbits (Oryctolagus cuniculus)
SUMMARY: The rabbit is considered an ideal animal model for studies that describe abnormalities in the testicles due to the similar morphogenetic mechanisms of sexual development and diseases commonly found in humans. The aim of this study was to determine the male sexual differentiation of the New...
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| Lenguaje: | English |
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Sociedad Chilena de Anatomía
2021
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oai:scielo:S0717-950220210005012532021-10-19Gonadal Differentiation During Embryonic and Fetal Development of Male New Zealand Rabbits (Oryctolagus cuniculus)Miranda,Carla Maria Figueiredo de CarvalhoLeonel,Luciano César Pereira CamposKoga,Bruna Andrade AguiarAnunciação,Adriana Raquel de Almeida daMario,Lara CarolinaFavaron,Phelipe OliveiraMartins,Daniele dos SantosVásquez,BélgicaConei,DanielMiglino,Maria Angélicadel Sol,Mariano Gonads Embryonic development Seminiferous tubules Desert hedgehog Sustentacular cells Primor- dial germ cells SUMMARY: The rabbit is considered an ideal animal model for studies that describe abnormalities in the testicles due to the similar morphogenetic mechanisms of sexual development and diseases commonly found in humans. The aim of this study was to determine the male sexual differentiation of the New Zealand rabbit (Oryctolagus cuniculus) through development. The gestational age was estimated and classified as 9, 12, 14, 16, 18, 20, 23 and 28 gestational days. The morphological and sexual determination were performed by histological analysis of the reproductive tract in the embryos and fetuses (9-28 days) as well as by immunohistochemistry- Desert hedgehog-Dhh- (testis-specific protein on Y chromosome- 16, 20, 23 days and adult rabbits). Gonads were observed from the 14th day in an undifferentiated stage and with homogeneous aspect. Sexual differentiation was observed from the 16th day with presence of cells forming gonadal cords and Dhh+ cells in the gonadal parenchyma. From the 18th gestational day testicular cords were observed, which evolved into organized seminiferous tubules. The formation of the efferent ducts and ductus deferens and epididymis was observed on the 20th and 23rd days, respectively. The differentiation of the external genitalia occurred from the 23rd days from the anogenital distance and was identified to identify the penile structures. In summary, the features of the sexual differentiation were determined by observation of the Dhh+ protein in embryos from the 16th day to adulthood, and the morphological particularities observed from the 18th gestational day, determined by differentiation of the external genitalia from the 23rd day.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.5 20212021-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000501253en10.4067/S0717-95022021000501253 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Gonads Embryonic development Seminiferous tubules Desert hedgehog Sustentacular cells Primor- dial germ cells |
| spellingShingle |
Gonads Embryonic development Seminiferous tubules Desert hedgehog Sustentacular cells Primor- dial germ cells Miranda,Carla Maria Figueiredo de Carvalho Leonel,Luciano César Pereira Campos Koga,Bruna Andrade Aguiar Anunciação,Adriana Raquel de Almeida da Mario,Lara Carolina Favaron,Phelipe Oliveira Martins,Daniele dos Santos Vásquez,Bélgica Conei,Daniel Miglino,Maria Angélica del Sol,Mariano Gonadal Differentiation During Embryonic and Fetal Development of Male New Zealand Rabbits (Oryctolagus cuniculus) |
| description |
SUMMARY: The rabbit is considered an ideal animal model for studies that describe abnormalities in the testicles due to the similar morphogenetic mechanisms of sexual development and diseases commonly found in humans. The aim of this study was to determine the male sexual differentiation of the New Zealand rabbit (Oryctolagus cuniculus) through development. The gestational age was estimated and classified as 9, 12, 14, 16, 18, 20, 23 and 28 gestational days. The morphological and sexual determination were performed by histological analysis of the reproductive tract in the embryos and fetuses (9-28 days) as well as by immunohistochemistry- Desert hedgehog-Dhh- (testis-specific protein on Y chromosome- 16, 20, 23 days and adult rabbits). Gonads were observed from the 14th day in an undifferentiated stage and with homogeneous aspect. Sexual differentiation was observed from the 16th day with presence of cells forming gonadal cords and Dhh+ cells in the gonadal parenchyma. From the 18th gestational day testicular cords were observed, which evolved into organized seminiferous tubules. The formation of the efferent ducts and ductus deferens and epididymis was observed on the 20th and 23rd days, respectively. The differentiation of the external genitalia occurred from the 23rd days from the anogenital distance and was identified to identify the penile structures. In summary, the features of the sexual differentiation were determined by observation of the Dhh+ protein in embryos from the 16th day to adulthood, and the morphological particularities observed from the 18th gestational day, determined by differentiation of the external genitalia from the 23rd day. |
| author |
Miranda,Carla Maria Figueiredo de Carvalho Leonel,Luciano César Pereira Campos Koga,Bruna Andrade Aguiar Anunciação,Adriana Raquel de Almeida da Mario,Lara Carolina Favaron,Phelipe Oliveira Martins,Daniele dos Santos Vásquez,Bélgica Conei,Daniel Miglino,Maria Angélica del Sol,Mariano |
| author_facet |
Miranda,Carla Maria Figueiredo de Carvalho Leonel,Luciano César Pereira Campos Koga,Bruna Andrade Aguiar Anunciação,Adriana Raquel de Almeida da Mario,Lara Carolina Favaron,Phelipe Oliveira Martins,Daniele dos Santos Vásquez,Bélgica Conei,Daniel Miglino,Maria Angélica del Sol,Mariano |
| author_sort |
Miranda,Carla Maria Figueiredo de Carvalho |
| title |
Gonadal Differentiation During Embryonic and Fetal Development of Male New Zealand Rabbits (Oryctolagus cuniculus) |
| title_short |
Gonadal Differentiation During Embryonic and Fetal Development of Male New Zealand Rabbits (Oryctolagus cuniculus) |
| title_full |
Gonadal Differentiation During Embryonic and Fetal Development of Male New Zealand Rabbits (Oryctolagus cuniculus) |
| title_fullStr |
Gonadal Differentiation During Embryonic and Fetal Development of Male New Zealand Rabbits (Oryctolagus cuniculus) |
| title_full_unstemmed |
Gonadal Differentiation During Embryonic and Fetal Development of Male New Zealand Rabbits (Oryctolagus cuniculus) |
| title_sort |
gonadal differentiation during embryonic and fetal development of male new zealand rabbits (oryctolagus cuniculus) |
| publisher |
Sociedad Chilena de Anatomía |
| publishDate |
2021 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000501253 |
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| _version_ |
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