Integrated Optical Density Analysis of the Immunohistochemical Expression of the Progesterone Receptor in the Uterine Endometrium of Prepubertal Araucana Sheep

SUMMARY: Progesterone receptors are expressed in the reproductive organs of adult sheep, where they regulate morphofunctional and reproductive development. However, various studies have shown the presence of these receptors in the uterus of prepubertal females. It is not clear what role these recept...

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Autores principales:	Loncoñanco,Ester, Navarrete,Felipe, Cuevas,Nicolás, Vasconcellos,Adriana, Paredes,Marco
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Sheep reproductive development Araucana sheep Integrated optical density
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000501278
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spelling	oai:scielo:S0717-950220210005012782021-10-19Integrated Optical Density Analysis of the Immunohistochemical Expression of the Progesterone Receptor in the Uterine Endometrium of Prepubertal Araucana SheepLoncoñanco,EsterNavarrete,FelipeCuevas,NicolásVasconcellos,AdrianaParedes,Marco Sheep reproductive development Araucana sheep Integrated optical density SUMMARY: Progesterone receptors are expressed in the reproductive organs of adult sheep, where they regulate morphofunctional and reproductive development. However, various studies have shown the presence of these receptors in the uterus of prepubertal females. It is not clear what role these receptors have at this level of development in uterine tissue. Therefore, it is relevant as a first step in the investigation, to determine the expression and histological distribution of the progesterone receptor in prepubertal sheep in order to determine possible functions at this level of reproductive development. Immunohistochemical analysis allows visualizing the specific presence of a protein in the cellular and histological context, however, the results displayed through digital images are qualitative data and subject to the observer's criteria. In this work, a quantitative analysis method of immunohistochemical expression of the progesterone receptor in ovine endometrium is presented, using digital analysis of images, by means of integrated optical density of digital photographs of histological sections processed with immunohistochemical methods. The results show the possibility of quantitatively evaluating the expression of progesterone receptors in the endometrial stroma and prepubertal endometrial glands by applying the integrated optical density analysis of digital images.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.5 20212021-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000501278en10.4067/S0717-95022021000501278
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Sheep reproductive development Araucana sheep Integrated optical density
spellingShingle	Sheep reproductive development Araucana sheep Integrated optical density Loncoñanco,Ester Navarrete,Felipe Cuevas,Nicolás Vasconcellos,Adriana Paredes,Marco Integrated Optical Density Analysis of the Immunohistochemical Expression of the Progesterone Receptor in the Uterine Endometrium of Prepubertal Araucana Sheep
description	SUMMARY: Progesterone receptors are expressed in the reproductive organs of adult sheep, where they regulate morphofunctional and reproductive development. However, various studies have shown the presence of these receptors in the uterus of prepubertal females. It is not clear what role these receptors have at this level of development in uterine tissue. Therefore, it is relevant as a first step in the investigation, to determine the expression and histological distribution of the progesterone receptor in prepubertal sheep in order to determine possible functions at this level of reproductive development. Immunohistochemical analysis allows visualizing the specific presence of a protein in the cellular and histological context, however, the results displayed through digital images are qualitative data and subject to the observer's criteria. In this work, a quantitative analysis method of immunohistochemical expression of the progesterone receptor in ovine endometrium is presented, using digital analysis of images, by means of integrated optical density of digital photographs of histological sections processed with immunohistochemical methods. The results show the possibility of quantitatively evaluating the expression of progesterone receptors in the endometrial stroma and prepubertal endometrial glands by applying the integrated optical density analysis of digital images.
author	Loncoñanco,Ester Navarrete,Felipe Cuevas,Nicolás Vasconcellos,Adriana Paredes,Marco
author_facet	Loncoñanco,Ester Navarrete,Felipe Cuevas,Nicolás Vasconcellos,Adriana Paredes,Marco
author_sort	Loncoñanco,Ester
title	Integrated Optical Density Analysis of the Immunohistochemical Expression of the Progesterone Receptor in the Uterine Endometrium of Prepubertal Araucana Sheep
title_short	Integrated Optical Density Analysis of the Immunohistochemical Expression of the Progesterone Receptor in the Uterine Endometrium of Prepubertal Araucana Sheep
title_full	Integrated Optical Density Analysis of the Immunohistochemical Expression of the Progesterone Receptor in the Uterine Endometrium of Prepubertal Araucana Sheep
title_fullStr	Integrated Optical Density Analysis of the Immunohistochemical Expression of the Progesterone Receptor in the Uterine Endometrium of Prepubertal Araucana Sheep
title_full_unstemmed	Integrated Optical Density Analysis of the Immunohistochemical Expression of the Progesterone Receptor in the Uterine Endometrium of Prepubertal Araucana Sheep
title_sort	integrated optical density analysis of the immunohistochemical expression of the progesterone receptor in the uterine endometrium of prepubertal araucana sheep
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000501278
work_keys_str_mv	AT lonconancoester integratedopticaldensityanalysisoftheimmunohistochemicalexpressionoftheprogesteronereceptorintheuterineendometriumofprepubertalaraucanasheep AT navarretefelipe integratedopticaldensityanalysisoftheimmunohistochemicalexpressionoftheprogesteronereceptorintheuterineendometriumofprepubertalaraucanasheep AT cuevasnicolas integratedopticaldensityanalysisoftheimmunohistochemicalexpressionoftheprogesteronereceptorintheuterineendometriumofprepubertalaraucanasheep AT vasconcellosadriana integratedopticaldensityanalysisoftheimmunohistochemicalexpressionoftheprogesteronereceptorintheuterineendometriumofprepubertalaraucanasheep AT paredesmarco integratedopticaldensityanalysisoftheimmunohistochemicalexpressionoftheprogesteronereceptorintheuterineendometriumofprepubertalaraucanasheep
_version_	1718445213597302784

Integrated Optical Density Analysis of the Immunohistochemical Expression of the Progesterone Receptor in the Uterine Endometrium of Prepubertal Araucana Sheep

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