Determination of the Relationship Among Cell Proliferation, Metabolic Activity and Stage of Pregnancy by AgNORs as Markers in Bovine Placenta

SUMMARY: Nucleolus Organizer Regions (NORs) are defined as nucleolar components containing argyrophilic proteins selectively stained by silver methods (AgNORs). Several investigations have shown the AgNOR quantity and area represent a valuable parameter of cell kinetics, since they reflect the level...

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Autores principales:	Carneiro,Patrícia dos Santos, Sasahara,Tais Harumi de Castro, Carvalho,Hianka Jasmyne Costa de, Miglino,Maria Angélica, Gomes,Silvio Pires
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Nucleolar organizer regions Placenta Cellular structures Pregnancy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000501358
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spelling	oai:scielo:S0717-950220210005013582021-10-19Determination of the Relationship Among Cell Proliferation, Metabolic Activity and Stage of Pregnancy by AgNORs as Markers in Bovine PlacentaCarneiro,Patrícia dos SantosSasahara,Tais Harumi de CastroCarvalho,Hianka Jasmyne Costa deMiglino,Maria AngélicaGomes,Silvio Pires Nucleolar organizer regions Placenta Cellular structures Pregnancy SUMMARY: Nucleolus Organizer Regions (NORs) are defined as nucleolar components containing argyrophilic proteins selectively stained by silver methods (AgNORs). Several investigations have shown the AgNOR quantity and area represent a valuable parameter of cell kinetics, since they reflect the level of activity and cellular proliferation. This article addresses an evaluation of the functional activity and relation between days of pregnancy and proliferative capacity of trophoblastic mononucleate and binucleate cells from bovine placentomes. Both the number and size of AgNORs were determined in different phases of gestation by silver nitrate staining in conventional histological slides. The results showed a significant increase (from 1 to 12 AgNORs) in the number of AgNORS per trophoblastic mononucleate cell in the 3rd trimester, with predominance of 4-6 AgNORs/cell. In the 1st and 2nd trimesters, the number ranged between 1 and 9 AgNORs/cell, with predominance of 1-3 AgNORs. No significant differences were observed between the 2nd and 3rd trimesters, but in the first, in binucleate cells (19-27 and 10-18 AgNORs/cell, respectively) - this number was higher than the one registered in trophoblastic mononucleate cells in the same period. Thus, AgNORs can be used as markers of the proliferative placental cell cycle and established a relation between number of AgNORs and days of gestation. This relation can be used for diagnoses and prognoses of several placental pathologies, including pregnancy losses from manipulated embryos.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.5 20212021-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000501358en10.4067/S0717-95022021000501358
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Nucleolar organizer regions Placenta Cellular structures Pregnancy
spellingShingle	Nucleolar organizer regions Placenta Cellular structures Pregnancy Carneiro,Patrícia dos Santos Sasahara,Tais Harumi de Castro Carvalho,Hianka Jasmyne Costa de Miglino,Maria Angélica Gomes,Silvio Pires Determination of the Relationship Among Cell Proliferation, Metabolic Activity and Stage of Pregnancy by AgNORs as Markers in Bovine Placenta
description	SUMMARY: Nucleolus Organizer Regions (NORs) are defined as nucleolar components containing argyrophilic proteins selectively stained by silver methods (AgNORs). Several investigations have shown the AgNOR quantity and area represent a valuable parameter of cell kinetics, since they reflect the level of activity and cellular proliferation. This article addresses an evaluation of the functional activity and relation between days of pregnancy and proliferative capacity of trophoblastic mononucleate and binucleate cells from bovine placentomes. Both the number and size of AgNORs were determined in different phases of gestation by silver nitrate staining in conventional histological slides. The results showed a significant increase (from 1 to 12 AgNORs) in the number of AgNORS per trophoblastic mononucleate cell in the 3rd trimester, with predominance of 4-6 AgNORs/cell. In the 1st and 2nd trimesters, the number ranged between 1 and 9 AgNORs/cell, with predominance of 1-3 AgNORs. No significant differences were observed between the 2nd and 3rd trimesters, but in the first, in binucleate cells (19-27 and 10-18 AgNORs/cell, respectively) - this number was higher than the one registered in trophoblastic mononucleate cells in the same period. Thus, AgNORs can be used as markers of the proliferative placental cell cycle and established a relation between number of AgNORs and days of gestation. This relation can be used for diagnoses and prognoses of several placental pathologies, including pregnancy losses from manipulated embryos.
author	Carneiro,Patrícia dos Santos Sasahara,Tais Harumi de Castro Carvalho,Hianka Jasmyne Costa de Miglino,Maria Angélica Gomes,Silvio Pires
author_facet	Carneiro,Patrícia dos Santos Sasahara,Tais Harumi de Castro Carvalho,Hianka Jasmyne Costa de Miglino,Maria Angélica Gomes,Silvio Pires
author_sort	Carneiro,Patrícia dos Santos
title	Determination of the Relationship Among Cell Proliferation, Metabolic Activity and Stage of Pregnancy by AgNORs as Markers in Bovine Placenta
title_short	Determination of the Relationship Among Cell Proliferation, Metabolic Activity and Stage of Pregnancy by AgNORs as Markers in Bovine Placenta
title_full	Determination of the Relationship Among Cell Proliferation, Metabolic Activity and Stage of Pregnancy by AgNORs as Markers in Bovine Placenta
title_fullStr	Determination of the Relationship Among Cell Proliferation, Metabolic Activity and Stage of Pregnancy by AgNORs as Markers in Bovine Placenta
title_full_unstemmed	Determination of the Relationship Among Cell Proliferation, Metabolic Activity and Stage of Pregnancy by AgNORs as Markers in Bovine Placenta
title_sort	determination of the relationship among cell proliferation, metabolic activity and stage of pregnancy by agnors as markers in bovine placenta
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000501358
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Determination of the Relationship Among Cell Proliferation, Metabolic Activity and Stage of Pregnancy by AgNORs as Markers in Bovine Placenta

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