Intrauterine Alcohol Exposure Disturbs Trabecular Morphology in the Sprague Dawley Rat Humerus Epiphysis up to 3- Weeks Postnatally

SUMMARY: Gestational alcohol exposure inhibits neurological as well as bone growth and development both in fetal and postnatal life. Stunted stature, osteoporosis and fractures in adult life are some of the adverse effects. While the impact of intrauterine alcohol on the brain has been extensively i...

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Autores principales:	Pillay,Diana, Ndou,Robert
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Microarchitecture Trabeculae Humerus Osteoporosis Prenatal alcohol
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000501436
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spelling	oai:scielo:S0717-950220210005014362021-10-19Intrauterine Alcohol Exposure Disturbs Trabecular Morphology in the Sprague Dawley Rat Humerus Epiphysis up to 3- Weeks PostnatallyPillay,DianaNdou,Robert Microarchitecture Trabeculae Humerus Osteoporosis Prenatal alcohol SUMMARY: Gestational alcohol exposure inhibits neurological as well as bone growth and development both in fetal and postnatal life. Stunted stature, osteoporosis and fractures in adult life are some of the adverse effects. While the impact of intrauterine alcohol on the brain has been extensively investigated, studies on the effects on bone are relatively few. Therefore, our study aimed to examine the impact of prenatal alcohol exposure on bone microarchitecture in 3-week-old rats using Micro-focus X-Ray Computed Tomography (Micro CT). Time mated pregnant Sprague Dawley dams (13) were randomly placed into 3 groups: ethanol (n=5), saline control (n=5) and untreated control (n=3). The former 2 groups received treatment with 0.015ml/g of 25.2 % ethanol and 0.9 % saline, respectively, for the first 19 days of gestation. The untreated group received no treatment. The pups remained with their dams until termination at 21 days of age. From each dam, 2 pups were collected resulting in: ethanol (n=10), saline controls (n= 10) and untreated controls (n = 6). The humeri of the pups were dissected and scanned using a 3D-μCT scanner (Nikon XTH 225L) at 15μm resolution. Trabecular and cortical parameters were analysed using Volume Graphics Studio® software following reconstruction. Results showed a decrease in trabecular size, spaces, thickness, and volume. There was a decrease in cortical bone area in the ethanol group compared to the controls. These findings may suggest that osteoporosis and fractures seen as gestational alcohol effects may be due to compromised trabecular structure.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.5 20212021-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000501436en10.4067/S0717-95022021000501436
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Microarchitecture Trabeculae Humerus Osteoporosis Prenatal alcohol
spellingShingle	Microarchitecture Trabeculae Humerus Osteoporosis Prenatal alcohol Pillay,Diana Ndou,Robert Intrauterine Alcohol Exposure Disturbs Trabecular Morphology in the Sprague Dawley Rat Humerus Epiphysis up to 3- Weeks Postnatally
description	SUMMARY: Gestational alcohol exposure inhibits neurological as well as bone growth and development both in fetal and postnatal life. Stunted stature, osteoporosis and fractures in adult life are some of the adverse effects. While the impact of intrauterine alcohol on the brain has been extensively investigated, studies on the effects on bone are relatively few. Therefore, our study aimed to examine the impact of prenatal alcohol exposure on bone microarchitecture in 3-week-old rats using Micro-focus X-Ray Computed Tomography (Micro CT). Time mated pregnant Sprague Dawley dams (13) were randomly placed into 3 groups: ethanol (n=5), saline control (n=5) and untreated control (n=3). The former 2 groups received treatment with 0.015ml/g of 25.2 % ethanol and 0.9 % saline, respectively, for the first 19 days of gestation. The untreated group received no treatment. The pups remained with their dams until termination at 21 days of age. From each dam, 2 pups were collected resulting in: ethanol (n=10), saline controls (n= 10) and untreated controls (n = 6). The humeri of the pups were dissected and scanned using a 3D-μCT scanner (Nikon XTH 225L) at 15μm resolution. Trabecular and cortical parameters were analysed using Volume Graphics Studio® software following reconstruction. Results showed a decrease in trabecular size, spaces, thickness, and volume. There was a decrease in cortical bone area in the ethanol group compared to the controls. These findings may suggest that osteoporosis and fractures seen as gestational alcohol effects may be due to compromised trabecular structure.
author	Pillay,Diana Ndou,Robert
author_facet	Pillay,Diana Ndou,Robert
author_sort	Pillay,Diana
title	Intrauterine Alcohol Exposure Disturbs Trabecular Morphology in the Sprague Dawley Rat Humerus Epiphysis up to 3- Weeks Postnatally
title_short	Intrauterine Alcohol Exposure Disturbs Trabecular Morphology in the Sprague Dawley Rat Humerus Epiphysis up to 3- Weeks Postnatally
title_full	Intrauterine Alcohol Exposure Disturbs Trabecular Morphology in the Sprague Dawley Rat Humerus Epiphysis up to 3- Weeks Postnatally
title_fullStr	Intrauterine Alcohol Exposure Disturbs Trabecular Morphology in the Sprague Dawley Rat Humerus Epiphysis up to 3- Weeks Postnatally
title_full_unstemmed	Intrauterine Alcohol Exposure Disturbs Trabecular Morphology in the Sprague Dawley Rat Humerus Epiphysis up to 3- Weeks Postnatally
title_sort	intrauterine alcohol exposure disturbs trabecular morphology in the sprague dawley rat humerus epiphysis up to 3- weeks postnatally
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000501436
work_keys_str_mv	AT pillaydiana intrauterinealcoholexposuredisturbstrabecularmorphologyinthespraguedawleyrathumerusepiphysisupto3weekspostnatally AT ndourobert intrauterinealcoholexposuredisturbstrabecularmorphologyinthespraguedawleyrathumerusepiphysisupto3weekspostnatally
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Intrauterine Alcohol Exposure Disturbs Trabecular Morphology in the Sprague Dawley Rat Humerus Epiphysis up to 3- Weeks Postnatally

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