Immunohistochemical Markers in the Differential Diagnosis of Melanoma and Nevus in Humans

SUMMARY: Immunohistochemistry allows in situ detection of cell and extracellular components through specific antibodies. The objective was to compare the immunohistochemical expression patterns of the S-100, HMB-45 and MART-1 proteins for differential diagnosis of malignant melanoma and melanocytic...

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Autores principales:	Quilaqueo,Nelson, Navarrete,Felipe, Sandoval,Cristian, Roa,Ignacio, Pellón,Mario, Paredes,Marco
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2021
Materias:	Antigen antibody complex Immunohistochemistry Immunohistochemical markers Pathology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000501509
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spelling	oai:scielo:S0717-950220210005015092021-10-19Immunohistochemical Markers in the Differential Diagnosis of Melanoma and Nevus in HumansQuilaqueo,NelsonNavarrete,FelipeSandoval,CristianRoa,IgnacioPellón,MarioParedes,Marco Antigen antibody complex Immunohistochemistry Immunohistochemical markers Pathology SUMMARY: Immunohistochemistry allows in situ detection of cell and extracellular components through specific antibodies. The objective was to compare the immunohistochemical expression patterns of the S-100, HMB-45 and MART-1 proteins for differential diagnosis of malignant melanoma and melanocytic nevus in human skin biopsies. Thirty-nine biopsies of human tissue were used. They were divided into two groups: 19 in malignant melanoma and 20 in melanocytic nevi. Next, the samples were fixed with paraformaldehyde and processed following the protocol for inclusion. Then, immunohistochemical staining was performed. Finally, the histological and qualitative analysis of the samples was carried out. S-100, HMB-45, and MART-1 markers showed positive immunoreaction in melanoma biopsies. HMB-45 marker was generally present with weaker expression than S-100 and MART-1 in melanocytic nevus biopsies. No expression pattern was observed which specifically associates one or more markers with some types of histopathological diagnosis. Immunohistochemistry is fundamental in differential diagnosis of melanomas and melanocytic nevi. However, there is no antibody or set of antibodies which allows unequivocal diagnosis between melanoma and nevus. It is therefore necessary to analyze with care the expression pattern and location of the lesion using standard morphological characteristics.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.39 n.5 20212021-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000501509en10.4067/S0717-95022021000501509
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antigen antibody complex Immunohistochemistry Immunohistochemical markers Pathology
spellingShingle	Antigen antibody complex Immunohistochemistry Immunohistochemical markers Pathology Quilaqueo,Nelson Navarrete,Felipe Sandoval,Cristian Roa,Ignacio Pellón,Mario Paredes,Marco Immunohistochemical Markers in the Differential Diagnosis of Melanoma and Nevus in Humans
description	SUMMARY: Immunohistochemistry allows in situ detection of cell and extracellular components through specific antibodies. The objective was to compare the immunohistochemical expression patterns of the S-100, HMB-45 and MART-1 proteins for differential diagnosis of malignant melanoma and melanocytic nevus in human skin biopsies. Thirty-nine biopsies of human tissue were used. They were divided into two groups: 19 in malignant melanoma and 20 in melanocytic nevi. Next, the samples were fixed with paraformaldehyde and processed following the protocol for inclusion. Then, immunohistochemical staining was performed. Finally, the histological and qualitative analysis of the samples was carried out. S-100, HMB-45, and MART-1 markers showed positive immunoreaction in melanoma biopsies. HMB-45 marker was generally present with weaker expression than S-100 and MART-1 in melanocytic nevus biopsies. No expression pattern was observed which specifically associates one or more markers with some types of histopathological diagnosis. Immunohistochemistry is fundamental in differential diagnosis of melanomas and melanocytic nevi. However, there is no antibody or set of antibodies which allows unequivocal diagnosis between melanoma and nevus. It is therefore necessary to analyze with care the expression pattern and location of the lesion using standard morphological characteristics.
author	Quilaqueo,Nelson Navarrete,Felipe Sandoval,Cristian Roa,Ignacio Pellón,Mario Paredes,Marco
author_facet	Quilaqueo,Nelson Navarrete,Felipe Sandoval,Cristian Roa,Ignacio Pellón,Mario Paredes,Marco
author_sort	Quilaqueo,Nelson
title	Immunohistochemical Markers in the Differential Diagnosis of Melanoma and Nevus in Humans
title_short	Immunohistochemical Markers in the Differential Diagnosis of Melanoma and Nevus in Humans
title_full	Immunohistochemical Markers in the Differential Diagnosis of Melanoma and Nevus in Humans
title_fullStr	Immunohistochemical Markers in the Differential Diagnosis of Melanoma and Nevus in Humans
title_full_unstemmed	Immunohistochemical Markers in the Differential Diagnosis of Melanoma and Nevus in Humans
title_sort	immunohistochemical markers in the differential diagnosis of melanoma and nevus in humans
publisher	Sociedad Chilena de Anatomía
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022021000501509
work_keys_str_mv	AT quilaqueonelson immunohistochemicalmarkersinthedifferentialdiagnosisofmelanomaandnevusinhumans AT navarretefelipe immunohistochemicalmarkersinthedifferentialdiagnosisofmelanomaandnevusinhumans AT sandovalcristian immunohistochemicalmarkersinthedifferentialdiagnosisofmelanomaandnevusinhumans AT roaignacio immunohistochemicalmarkersinthedifferentialdiagnosisofmelanomaandnevusinhumans AT pellonmario immunohistochemicalmarkersinthedifferentialdiagnosisofmelanomaandnevusinhumans AT paredesmarco immunohistochemicalmarkersinthedifferentialdiagnosisofmelanomaandnevusinhumans
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Immunohistochemical Markers in the Differential Diagnosis of Melanoma and Nevus in Humans

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