METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

A practical reversed-phase high-performance liquid chromatography (HPLC) method was developed for 4-chloro-N-furfuryl-5-sulphamoylanthranilic acid (furosemide) determination in plasma. The plasma samples spiked with the drug in presence of propranolol hydrochloride as internal standard, were purifie...

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Autores principales:	GOMEZ G,CAROLINA, PLESSING R,CARLOS von, GODOY M,C. GLORIA, REINBACH H,ROLANDO, GODOY R,RICARDO
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2005
Materias:	4-chloro-N-furfuryl-5-sulphamoylanthranilic acid urosemide liquid-liquid extraction from plasma HPLC with fluorescence detection validation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072005000200008
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spelling	oai:scielo:S0717-970720050002000082005-07-19METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTIONGOMEZ G,CAROLINAPLESSING R,CARLOS vonGODOY M,C. GLORIAREINBACH H,ROLANDOGODOY R,RICARDO 4-chloro-N-furfuryl-5-sulphamoylanthranilic acid urosemide liquid-liquid extraction from plasma HPLC with fluorescence detection validation A practical reversed-phase high-performance liquid chromatography (HPLC) method was developed for 4-chloro-N-furfuryl-5-sulphamoylanthranilic acid (furosemide) determination in plasma. The plasma samples spiked with the drug in presence of propranolol hydrochloride as internal standard, were purified using a liquid - liquid extraction technique. The drugs were separated through a Kromasil 100-5C18 column with a mobile phase composed of acetonitrile and 0.02 M potassium dihydrogen phosphate (34 : 66 v/v) adjusted to pH 3.0 and quantified by fluorescence detection (excitation at 268 nm, emission at 410 nm). The calibration curve was linear ranging between 0.005 and 1.500 mg/mL. The detection limit was 0.001mg/mL and the quantification limit 0.003 mg/mL in plasma. The furosemide recovery from 1mL of plasma was between 99.8 and 101.6 %, with a variation coefficient not higher than 4,85 %. The method is sensitive enough for use in bioavailability and bioequivalence studies after oral administration of 40 mg (a tablet) of furosemideinfo:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.50 n.2 20052005-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072005000200008en10.4067/S0717-97072005000200008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	4-chloro-N-furfuryl-5-sulphamoylanthranilic acid urosemide liquid-liquid extraction from plasma HPLC with fluorescence detection validation
spellingShingle	4-chloro-N-furfuryl-5-sulphamoylanthranilic acid urosemide liquid-liquid extraction from plasma HPLC with fluorescence detection validation GOMEZ G,CAROLINA PLESSING R,CARLOS von GODOY M,C. GLORIA REINBACH H,ROLANDO GODOY R,RICARDO METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
description	A practical reversed-phase high-performance liquid chromatography (HPLC) method was developed for 4-chloro-N-furfuryl-5-sulphamoylanthranilic acid (furosemide) determination in plasma. The plasma samples spiked with the drug in presence of propranolol hydrochloride as internal standard, were purified using a liquid - liquid extraction technique. The drugs were separated through a Kromasil 100-5C18 column with a mobile phase composed of acetonitrile and 0.02 M potassium dihydrogen phosphate (34 : 66 v/v) adjusted to pH 3.0 and quantified by fluorescence detection (excitation at 268 nm, emission at 410 nm). The calibration curve was linear ranging between 0.005 and 1.500 mg/mL. The detection limit was 0.001mg/mL and the quantification limit 0.003 mg/mL in plasma. The furosemide recovery from 1mL of plasma was between 99.8 and 101.6 %, with a variation coefficient not higher than 4,85 %. The method is sensitive enough for use in bioavailability and bioequivalence studies after oral administration of 40 mg (a tablet) of furosemide
author	GOMEZ G,CAROLINA PLESSING R,CARLOS von GODOY M,C. GLORIA REINBACH H,ROLANDO GODOY R,RICARDO
author_facet	GOMEZ G,CAROLINA PLESSING R,CARLOS von GODOY M,C. GLORIA REINBACH H,ROLANDO GODOY R,RICARDO
author_sort	GOMEZ G,CAROLINA
title	METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
title_short	METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
title_full	METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
title_fullStr	METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
title_full_unstemmed	METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
title_sort	method validation for the determination of furosemide in plasma by liquid- liquid extraction and high-performance liquid chromatography with fluorescence detection
publisher	Sociedad Chilena de Química
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072005000200008
work_keys_str_mv	AT gomezgcarolina methodvalidationforthedeterminationoffurosemideinplasmabyliquidliquidextractionandhighperformanceliquidchromatographywithfluorescencedetection AT plessingrcarlosvon methodvalidationforthedeterminationoffurosemideinplasmabyliquidliquidextractionandhighperformanceliquidchromatographywithfluorescencedetection AT godoymcgloria methodvalidationforthedeterminationoffurosemideinplasmabyliquidliquidextractionandhighperformanceliquidchromatographywithfluorescencedetection AT reinbachhrolando methodvalidationforthedeterminationoffurosemideinplasmabyliquidliquidextractionandhighperformanceliquidchromatographywithfluorescencedetection AT godoyrricardo methodvalidationforthedeterminationoffurosemideinplasmabyliquidliquidextractionandhighperformanceliquidchromatographywithfluorescencedetection
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METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

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