CARBOHYDRATES FROM THE SEQUENTIAL EXTRACTION OF Lessonia vadosa (PHAEOPHYTA)
Blades of Lessonia vadosa were sequentially extracted with 70% ethanol, followed by 2% CaCl2 solution,, diluted HCl (pH 2), and finally with 2% Na2CO3 solution. D-mannitol was the only low molecular weight carbohydrate obtained from the ethanolic extract. By ion-exhange chromatography of the CaCl2 e...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Sociedad Chilena de Química
2005
|
| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072005000200012 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: | Blades of Lessonia vadosa were sequentially extracted with 70% ethanol, followed by 2% CaCl2 solution,, diluted HCl (pH 2), and finally with 2% Na2CO3 solution. D-mannitol was the only low molecular weight carbohydrate obtained from the ethanolic extract. By ion-exhange chromatography of the CaCl2 extract a fraction contaning 36.7% of sulfate group was obtained. Chemical and spectroscopic analysis indicated the presence of a regular polymer of a-L-fucopyranose linked 1Æ3 and sulfated at positions O-2 and O-4. The main fraction isolated from the acidic extract by ion-exchange chromatography was shown to be composed of a complex a-L-fucan with 28.9% of sulfate. Alginic acid (17.0% of dry algae) was the major polysaccharide obtained in the sequential extraction |
|---|