CARBOHYDRATES FROM THE SEQUENTIAL EXTRACTION OF Lessonia vadosa (PHAEOPHYTA)

Blades of Lessonia vadosa were sequentially extracted with 70% ethanol, followed by 2% CaCl2 solution,, diluted HCl (pH 2), and finally with 2% Na2CO3 solution. D-mannitol was the only low molecular weight carbohydrate obtained from the ethanolic extract. By ion-exhange chromatography of the CaCl2 e...

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Autores principales:	CHANDIA,NANCY P., MATSUHIRO,BETTY, ORTIZ,JOHANNA S., MANSILLA,ANDRES
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2005
Materias:	Lessonia vadosa alginic acid mannitol sulfated fucans
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072005000200012
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spelling	oai:scielo:S0717-970720050002000122005-07-19CARBOHYDRATES FROM THE SEQUENTIAL EXTRACTION OF Lessonia vadosa (PHAEOPHYTA)CHANDIA,NANCY P.MATSUHIRO,BETTYORTIZ,JOHANNA S.MANSILLA,ANDRES Lessonia vadosa alginic acid mannitol sulfated fucans Blades of Lessonia vadosa were sequentially extracted with 70% ethanol, followed by 2% CaCl2 solution,, diluted HCl (pH 2), and finally with 2% Na2CO3 solution. D-mannitol was the only low molecular weight carbohydrate obtained from the ethanolic extract. By ion-exhange chromatography of the CaCl2 extract a fraction contaning 36.7% of sulfate group was obtained. Chemical and spectroscopic analysis indicated the presence of a regular polymer of a-L-fucopyranose linked 1Æ3 and sulfated at positions O-2 and O-4. The main fraction isolated from the acidic extract by ion-exchange chromatography was shown to be composed of a complex a-L-fucan with 28.9% of sulfate. Alginic acid (17.0% of dry algae) was the major polysaccharide obtained in the sequential extractioninfo:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.50 n.2 20052005-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072005000200012en10.4067/S0717-97072005000200012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Lessonia vadosa alginic acid mannitol sulfated fucans
spellingShingle	Lessonia vadosa alginic acid mannitol sulfated fucans CHANDIA,NANCY P. MATSUHIRO,BETTY ORTIZ,JOHANNA S. MANSILLA,ANDRES CARBOHYDRATES FROM THE SEQUENTIAL EXTRACTION OF Lessonia vadosa (PHAEOPHYTA)
description	Blades of Lessonia vadosa were sequentially extracted with 70% ethanol, followed by 2% CaCl2 solution,, diluted HCl (pH 2), and finally with 2% Na2CO3 solution. D-mannitol was the only low molecular weight carbohydrate obtained from the ethanolic extract. By ion-exhange chromatography of the CaCl2 extract a fraction contaning 36.7% of sulfate group was obtained. Chemical and spectroscopic analysis indicated the presence of a regular polymer of a-L-fucopyranose linked 1Æ3 and sulfated at positions O-2 and O-4. The main fraction isolated from the acidic extract by ion-exchange chromatography was shown to be composed of a complex a-L-fucan with 28.9% of sulfate. Alginic acid (17.0% of dry algae) was the major polysaccharide obtained in the sequential extraction
author	CHANDIA,NANCY P. MATSUHIRO,BETTY ORTIZ,JOHANNA S. MANSILLA,ANDRES
author_facet	CHANDIA,NANCY P. MATSUHIRO,BETTY ORTIZ,JOHANNA S. MANSILLA,ANDRES
author_sort	CHANDIA,NANCY P.
title	CARBOHYDRATES FROM THE SEQUENTIAL EXTRACTION OF Lessonia vadosa (PHAEOPHYTA)
title_short	CARBOHYDRATES FROM THE SEQUENTIAL EXTRACTION OF Lessonia vadosa (PHAEOPHYTA)
title_full	CARBOHYDRATES FROM THE SEQUENTIAL EXTRACTION OF Lessonia vadosa (PHAEOPHYTA)
title_fullStr	CARBOHYDRATES FROM THE SEQUENTIAL EXTRACTION OF Lessonia vadosa (PHAEOPHYTA)
title_full_unstemmed	CARBOHYDRATES FROM THE SEQUENTIAL EXTRACTION OF Lessonia vadosa (PHAEOPHYTA)
title_sort	carbohydrates from the sequential extraction of lessonia vadosa (phaeophyta)
publisher	Sociedad Chilena de Química
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072005000200012
work_keys_str_mv	AT chandianancyp carbohydratesfromthesequentialextractionoflessoniavadosaphaeophyta AT matsuhirobetty carbohydratesfromthesequentialextractionoflessoniavadosaphaeophyta AT ortizjohannas carbohydratesfromthesequentialextractionoflessoniavadosaphaeophyta AT mansillaandres carbohydratesfromthesequentialextractionoflessoniavadosaphaeophyta
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CARBOHYDRATES FROM THE SEQUENTIAL EXTRACTION OF Lessonia vadosa (PHAEOPHYTA)

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