NEW TITRIMETRIC AND SPECTROPHOTOMETRIC METHODS FOR THE ASSAY OF PROCHLORPERAZINE IN PHARMACEUTICALS USING N-CHLOROSUCCINIMIDE AND TWO DYES

One titrimetric and two spectrophotometric methods are described for the assay of prochlorperazine mesylate(PCPM) in bulk drug and in injection using N-chlorosuccinimide(NCS) and two dyes, methyl orange and indigo carmine as reagents. In titrimetry, aqueous solution of PCPM is titrated directly with...

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Autores principales:	BASAVAIAH,K, SOMASHEKAR,B.C
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2007
Materias:	Prochlorperazine N-chlorosuccinimide Titrimetry Spectrophotometry Pharmaceuticals
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072007000400005
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spelling	oai:scielo:S0717-970720070004000052008-04-30NEW TITRIMETRIC AND SPECTROPHOTOMETRIC METHODS FOR THE ASSAY OF PROCHLORPERAZINE IN PHARMACEUTICALS USING N-CHLOROSUCCINIMIDE AND TWO DYESBASAVAIAH,KSOMASHEKAR,B.C Prochlorperazine N-chlorosuccinimide Titrimetry Spectrophotometry Pharmaceuticals One titrimetric and two spectrophotometric methods are described for the assay of prochlorperazine mesylate(PCPM) in bulk drug and in injection using N-chlorosuccinimide(NCS) and two dyes, methyl orange and indigo carmine as reagents. In titrimetry, aqueous solution of PCPM is titrated directly with a standard solution of NCS in acid medium and in the presence of potassium bromide(KBr). Spectrophotometric methods entail the addition of a known excess of NCS to a solution of PCPM in acid medium and in the presence of KBr followed by determination of residual bromine by reacting with a fixed amount of either methyl orange and measuring the absorbance at 520 nm (method A) or indigo carmine and measuring the absorbance at 610 nm (method B). In all the methods, the amount of NCS reacted corresponds to the amount of PCPM. In titrimetry, the reaction stoichiometry was found to be 1:1 (PCPM: NCS) and is applicable over 3.5-20 mg range. In spectrophotometric methods, the absorbance was found increase linearly with PCPM concentration with the correlation coefficients of 0.9986 and 0.9993 for method A and method B, respectively. The systems obey Beer's law for 0.25-3.0 µg/mL (method A) and 0.5-8.0 µg/mL (method B). The calculated apparent molar absorptivity values are 1.11 x10(5) and 4.49 x10(4) L/mol/cm for method A and method B, respectively, and the corresponding Sandell sensitivity values are 0.005 and 0.013 ug/cm². The limits of detection and quantification are reported for both spectrophotometric methods. Intra-day and inter-day precision and accuracy of the methods were evaluated as per ICH guidelines. The methods were successfully applied to the determination of PCPM in injection, and the results were statistically compared with those of a reference method by applying Student's t- test and F-test. No interference was observed from common additives found in injection solution. The accuracy and reliability of the methods were further ascertained by performing recovery test via standard addition methodinfo:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.52 n.4 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072007000400005en10.4067/S0717-97072007000400005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Prochlorperazine N-chlorosuccinimide Titrimetry Spectrophotometry Pharmaceuticals
spellingShingle	Prochlorperazine N-chlorosuccinimide Titrimetry Spectrophotometry Pharmaceuticals BASAVAIAH,K SOMASHEKAR,B.C NEW TITRIMETRIC AND SPECTROPHOTOMETRIC METHODS FOR THE ASSAY OF PROCHLORPERAZINE IN PHARMACEUTICALS USING N-CHLOROSUCCINIMIDE AND TWO DYES
description	One titrimetric and two spectrophotometric methods are described for the assay of prochlorperazine mesylate(PCPM) in bulk drug and in injection using N-chlorosuccinimide(NCS) and two dyes, methyl orange and indigo carmine as reagents. In titrimetry, aqueous solution of PCPM is titrated directly with a standard solution of NCS in acid medium and in the presence of potassium bromide(KBr). Spectrophotometric methods entail the addition of a known excess of NCS to a solution of PCPM in acid medium and in the presence of KBr followed by determination of residual bromine by reacting with a fixed amount of either methyl orange and measuring the absorbance at 520 nm (method A) or indigo carmine and measuring the absorbance at 610 nm (method B). In all the methods, the amount of NCS reacted corresponds to the amount of PCPM. In titrimetry, the reaction stoichiometry was found to be 1:1 (PCPM: NCS) and is applicable over 3.5-20 mg range. In spectrophotometric methods, the absorbance was found increase linearly with PCPM concentration with the correlation coefficients of 0.9986 and 0.9993 for method A and method B, respectively. The systems obey Beer's law for 0.25-3.0 µg/mL (method A) and 0.5-8.0 µg/mL (method B). The calculated apparent molar absorptivity values are 1.11 x10(5) and 4.49 x10(4) L/mol/cm for method A and method B, respectively, and the corresponding Sandell sensitivity values are 0.005 and 0.013 ug/cm². The limits of detection and quantification are reported for both spectrophotometric methods. Intra-day and inter-day precision and accuracy of the methods were evaluated as per ICH guidelines. The methods were successfully applied to the determination of PCPM in injection, and the results were statistically compared with those of a reference method by applying Student's t- test and F-test. No interference was observed from common additives found in injection solution. The accuracy and reliability of the methods were further ascertained by performing recovery test via standard addition method
author	BASAVAIAH,K SOMASHEKAR,B.C
author_facet	BASAVAIAH,K SOMASHEKAR,B.C
author_sort	BASAVAIAH,K
title	NEW TITRIMETRIC AND SPECTROPHOTOMETRIC METHODS FOR THE ASSAY OF PROCHLORPERAZINE IN PHARMACEUTICALS USING N-CHLOROSUCCINIMIDE AND TWO DYES
title_short	NEW TITRIMETRIC AND SPECTROPHOTOMETRIC METHODS FOR THE ASSAY OF PROCHLORPERAZINE IN PHARMACEUTICALS USING N-CHLOROSUCCINIMIDE AND TWO DYES
title_full	NEW TITRIMETRIC AND SPECTROPHOTOMETRIC METHODS FOR THE ASSAY OF PROCHLORPERAZINE IN PHARMACEUTICALS USING N-CHLOROSUCCINIMIDE AND TWO DYES
title_fullStr	NEW TITRIMETRIC AND SPECTROPHOTOMETRIC METHODS FOR THE ASSAY OF PROCHLORPERAZINE IN PHARMACEUTICALS USING N-CHLOROSUCCINIMIDE AND TWO DYES
title_full_unstemmed	NEW TITRIMETRIC AND SPECTROPHOTOMETRIC METHODS FOR THE ASSAY OF PROCHLORPERAZINE IN PHARMACEUTICALS USING N-CHLOROSUCCINIMIDE AND TWO DYES
title_sort	new titrimetric and spectrophotometric methods for the assay of prochlorperazine in pharmaceuticals using n-chlorosuccinimide and two dyes
publisher	Sociedad Chilena de Química
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072007000400005
work_keys_str_mv	AT basavaiahk newtitrimetricandspectrophotometricmethodsfortheassayofprochlorperazineinpharmaceuticalsusingnchlorosuccinimideandtwodyes AT somashekarbc newtitrimetricandspectrophotometricmethodsfortheassayofprochlorperazineinpharmaceuticalsusingnchlorosuccinimideandtwodyes
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NEW TITRIMETRIC AND SPECTROPHOTOMETRIC METHODS FOR THE ASSAY OF PROCHLORPERAZINE IN PHARMACEUTICALS USING N-CHLOROSUCCINIMIDE AND TWO DYES

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