AMYLOSE/AMYLOPECTIN SIMPLE DETERMINATION IN ACID HYDROLYZED TAPIOCA STARCH
Analysis of the shift of wavelength máximum using a rapid colorimetric method was used to determine the ratio of amylose:amylopectin (Am:Ap) in acid-hydrolyzed tapioca starch. The absorbance máximum of 600 nm (Am:Ap of tapioca starch ≈ 22:78) moved to shorter wavelengths (590, 585, 570, 56...
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| Autores principales: | , , , |
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| Lenguaje: | English |
| Publicado: |
Sociedad Chilena de Química
2008
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| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072008000300002 |
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| Sumario: | Analysis of the shift of wavelength máximum using a rapid colorimetric method was used to determine the ratio of amylose:amylopectin (Am:Ap) in acid-hydrolyzed tapioca starch. The absorbance máximum of 600 nm (Am:Ap of tapioca starch ≈ 22:78) moved to shorter wavelengths (590, 585, 570, 560, and 534 nm) as the decrease of the Am:Ap ratio due to hydrolysis of shorter chains that are not be able to form a complex with iodine. The amount of amylopectin itself may be unaltered or slightly decreased but the decrease in amylose caused a decrease in Am:Ap ratio |
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