SPECTROPHOTOMETRIC STUDIES ON THE INTERACTION OF YEAST RNA WITH CRYSTAL VIOLET AND ITS ANALYTICAL APPLICATION

The interaction of crystal violet (CV) with yeast RNA (yRNA) was studied by UV-Vis absorption spectrophotometric method in this paper and further a simple spectrophotometric method for the yRNA determination was proposed. At a pH 4.0 Britton-Robinson (B-R) buffer solution, CV had a máximum absorptio...

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Autores principales:	ZHAO,NA, WANG,MANXIA, NIU,XUELIANG, SUN,WEI, JIAO,KUI
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2008
Materias:	crystal violet yeast RNA UV-Vis spectrophotometry interaction
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072008000300009
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spelling	oai:scielo:S0717-970720080003000092008-10-23SPECTROPHOTOMETRIC STUDIES ON THE INTERACTION OF YEAST RNA WITH CRYSTAL VIOLET AND ITS ANALYTICAL APPLICATIONZHAO,NAWANG,MANXIANIU,XUELIANGSUN,WEIJIAO,KUI crystal violet yeast RNA UV-Vis spectrophotometry interaction The interaction of crystal violet (CV) with yeast RNA (yRNA) was studied by UV-Vis absorption spectrophotometric method in this paper and further a simple spectrophotometric method for the yRNA determination was proposed. At a pH 4.0 Britton-Robinson (B-R) buffer solution, CV had a máximum absorption peak at 590 nm, which was greatly decreased by the addition of yRNA. The conditions of CVinteracted with yRNA were carefully investigated. Underthe optimal conditions the decrease of absorbance value was proportional to the concentration of yRNA in the range from 3.33x10-6 to 1.67x10-5 mol L-1 when 2.0 x 10(5) mol L-1 CV was employed. The detection limit was calculated as 5.34 x 10-7 mol L-1 (3σ) and different samples were determined satisfactorily. The binding number and the binding constant of CV with yRNA were calculated by spectrophotometric data with the results as 1 and 1.69 x 10(5) L mol-1, respectively. The reaction mechanism was discussed with the electrostatic attraction led to molecular association of yRNA with CVinfo:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.53 n.3 20082008-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072008000300009en10.4067/S0717-97072008000300009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	crystal violet yeast RNA UV-Vis spectrophotometry interaction
spellingShingle	crystal violet yeast RNA UV-Vis spectrophotometry interaction ZHAO,NA WANG,MANXIA NIU,XUELIANG SUN,WEI JIAO,KUI SPECTROPHOTOMETRIC STUDIES ON THE INTERACTION OF YEAST RNA WITH CRYSTAL VIOLET AND ITS ANALYTICAL APPLICATION
description	The interaction of crystal violet (CV) with yeast RNA (yRNA) was studied by UV-Vis absorption spectrophotometric method in this paper and further a simple spectrophotometric method for the yRNA determination was proposed. At a pH 4.0 Britton-Robinson (B-R) buffer solution, CV had a máximum absorption peak at 590 nm, which was greatly decreased by the addition of yRNA. The conditions of CVinteracted with yRNA were carefully investigated. Underthe optimal conditions the decrease of absorbance value was proportional to the concentration of yRNA in the range from 3.33x10-6 to 1.67x10-5 mol L-1 when 2.0 x 10(5) mol L-1 CV was employed. The detection limit was calculated as 5.34 x 10-7 mol L-1 (3σ) and different samples were determined satisfactorily. The binding number and the binding constant of CV with yRNA were calculated by spectrophotometric data with the results as 1 and 1.69 x 10(5) L mol-1, respectively. The reaction mechanism was discussed with the electrostatic attraction led to molecular association of yRNA with CV
author	ZHAO,NA WANG,MANXIA NIU,XUELIANG SUN,WEI JIAO,KUI
author_facet	ZHAO,NA WANG,MANXIA NIU,XUELIANG SUN,WEI JIAO,KUI
author_sort	ZHAO,NA
title	SPECTROPHOTOMETRIC STUDIES ON THE INTERACTION OF YEAST RNA WITH CRYSTAL VIOLET AND ITS ANALYTICAL APPLICATION
title_short	SPECTROPHOTOMETRIC STUDIES ON THE INTERACTION OF YEAST RNA WITH CRYSTAL VIOLET AND ITS ANALYTICAL APPLICATION
title_full	SPECTROPHOTOMETRIC STUDIES ON THE INTERACTION OF YEAST RNA WITH CRYSTAL VIOLET AND ITS ANALYTICAL APPLICATION
title_fullStr	SPECTROPHOTOMETRIC STUDIES ON THE INTERACTION OF YEAST RNA WITH CRYSTAL VIOLET AND ITS ANALYTICAL APPLICATION
title_full_unstemmed	SPECTROPHOTOMETRIC STUDIES ON THE INTERACTION OF YEAST RNA WITH CRYSTAL VIOLET AND ITS ANALYTICAL APPLICATION
title_sort	spectrophotometric studies on the interaction of yeast rna with crystal violet and its analytical application
publisher	Sociedad Chilena de Química
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072008000300009
work_keys_str_mv	AT zhaona spectrophotometricstudiesontheinteractionofyeastrnawithcrystalvioletanditsanalyticalapplication AT wangmanxia spectrophotometricstudiesontheinteractionofyeastrnawithcrystalvioletanditsanalyticalapplication AT niuxueliang spectrophotometricstudiesontheinteractionofyeastrnawithcrystalvioletanditsanalyticalapplication AT sunwei spectrophotometricstudiesontheinteractionofyeastrnawithcrystalvioletanditsanalyticalapplication AT jiaokui spectrophotometricstudiesontheinteractionofyeastrnawithcrystalvioletanditsanalyticalapplication
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SPECTROPHOTOMETRIC STUDIES ON THE INTERACTION OF YEAST RNA WITH CRYSTAL VIOLET AND ITS ANALYTICAL APPLICATION

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