SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND GLUTATHIONE DISULFIDE IN AN ACID EXTRACT OF PLANT SHOOT AND ROOT BY CAPILLARY ELECTROPHORESIS

This study describes the fast and simultaneous determination of glutathione and glutathione disulfide by Capillary Zone Electrophoresis in plant extracts of shoot and root of tomato plants. Frequentuse of acidic precipitation of protein generates an acidic matrixof strength and pH tha may cause chan...

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Autores principales:	MENDOZA,JORGE, GARRIDO,TATIANA, RIVEROS,RAÚL, GONZÁLEZ,CECILIA, PARADA,JOSÉ
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2008
Materias:	Glutathione Capillary Electrophoresis Metaphosphoric acid Acidic precipitation Glutathione disulfide
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072008000300018
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spelling	oai:scielo:S0717-970720080003000182008-10-23SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND GLUTATHIONE DISULFIDE IN AN ACID EXTRACT OF PLANT SHOOT AND ROOT BY CAPILLARY ELECTROPHORESISMENDOZA,JORGEGARRIDO,TATIANARIVEROS,RAÚLGONZÁLEZ,CECILIAPARADA,JOSÉ Glutathione Capillary Electrophoresis Metaphosphoric acid Acidic precipitation Glutathione disulfide This study describes the fast and simultaneous determination of glutathione and glutathione disulfide by Capillary Zone Electrophoresis in plant extracts of shoot and root of tomato plants. Frequentuse of acidic precipitation of protein generates an acidic matrixof strength and pH tha may cause changes inthe method sensitivity, comigration of species or changes in the equilibria that relate both species in cells or fluids. In this study, the resulting acidic matrix was previously treated with the same background electrolyte to prevent comigration and to improve signal resolution. Optimization of some parameters of the technique allowed the determination of both analytes in less than three minutes. The optimized method showed good reproducibility and linearity, with correlation coefficients above 0.999 and detection limits below 3 µM for both peptides. Analyte recovery in the process was in the 88-104% range. The concentration found in tomato plants hydroponically grown inthe absence of stress factors was inthe 51-100 nmol g-1 range, fresh weight for GSH and 5-32 nmol g-1 range, fresh weightfor GSSGinfo:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.53 n.3 20082008-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072008000300018en10.4067/S0717-97072008000300018
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Glutathione Capillary Electrophoresis Metaphosphoric acid Acidic precipitation Glutathione disulfide
spellingShingle	Glutathione Capillary Electrophoresis Metaphosphoric acid Acidic precipitation Glutathione disulfide MENDOZA,JORGE GARRIDO,TATIANA RIVEROS,RAÚL GONZÁLEZ,CECILIA PARADA,JOSÉ SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND GLUTATHIONE DISULFIDE IN AN ACID EXTRACT OF PLANT SHOOT AND ROOT BY CAPILLARY ELECTROPHORESIS
description	This study describes the fast and simultaneous determination of glutathione and glutathione disulfide by Capillary Zone Electrophoresis in plant extracts of shoot and root of tomato plants. Frequentuse of acidic precipitation of protein generates an acidic matrixof strength and pH tha may cause changes inthe method sensitivity, comigration of species or changes in the equilibria that relate both species in cells or fluids. In this study, the resulting acidic matrix was previously treated with the same background electrolyte to prevent comigration and to improve signal resolution. Optimization of some parameters of the technique allowed the determination of both analytes in less than three minutes. The optimized method showed good reproducibility and linearity, with correlation coefficients above 0.999 and detection limits below 3 µM for both peptides. Analyte recovery in the process was in the 88-104% range. The concentration found in tomato plants hydroponically grown inthe absence of stress factors was inthe 51-100 nmol g-1 range, fresh weight for GSH and 5-32 nmol g-1 range, fresh weightfor GSSG
author	MENDOZA,JORGE GARRIDO,TATIANA RIVEROS,RAÚL GONZÁLEZ,CECILIA PARADA,JOSÉ
author_facet	MENDOZA,JORGE GARRIDO,TATIANA RIVEROS,RAÚL GONZÁLEZ,CECILIA PARADA,JOSÉ
author_sort	MENDOZA,JORGE
title	SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND GLUTATHIONE DISULFIDE IN AN ACID EXTRACT OF PLANT SHOOT AND ROOT BY CAPILLARY ELECTROPHORESIS
title_short	SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND GLUTATHIONE DISULFIDE IN AN ACID EXTRACT OF PLANT SHOOT AND ROOT BY CAPILLARY ELECTROPHORESIS
title_full	SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND GLUTATHIONE DISULFIDE IN AN ACID EXTRACT OF PLANT SHOOT AND ROOT BY CAPILLARY ELECTROPHORESIS
title_fullStr	SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND GLUTATHIONE DISULFIDE IN AN ACID EXTRACT OF PLANT SHOOT AND ROOT BY CAPILLARY ELECTROPHORESIS
title_full_unstemmed	SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND GLUTATHIONE DISULFIDE IN AN ACID EXTRACT OF PLANT SHOOT AND ROOT BY CAPILLARY ELECTROPHORESIS
title_sort	simultaneous determination of glutathione and glutathione disulfide in an acid extract of plant shoot and root by capillary electrophoresis
publisher	Sociedad Chilena de Química
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072008000300018
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SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND GLUTATHIONE DISULFIDE IN AN ACID EXTRACT OF PLANT SHOOT AND ROOT BY CAPILLARY ELECTROPHORESIS

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