SCAVENGING ACTIVITY OF DICLOFENAC: INTERACTION WITH ABTS RADICAL CATION AND PEROXYL RADICALS

The scavenging activity of diclofenac was estimated by its capability to bleach ABTS radical cation, and ORAC (oxygen radical absorbance capacity) methodology. In addition, GC-MS technique was used to establish if the lactamic derivative of diclofenac was formed as final product in the interaction w...

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Autores principales:	ROJO,C, ÁLVAREZ-FIGUEROA,M. J, SOTO,M, CAÑETE,A, PESSOA-MAHANA,D, LÓPEZ-ALARCÓN,C
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2009
Materias:	Diclofenac fluorescein c-phycocyanin ORAC scavenging activity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000100014
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spelling	oai:scielo:S0717-970720090001000142009-05-12SCAVENGING ACTIVITY OF DICLOFENAC: INTERACTION WITH ABTS RADICAL CATION AND PEROXYL RADICALSROJO,CÁLVAREZ-FIGUEROA,M. JSOTO,MCAÑETE,APESSOA-MAHANA,DLÓPEZ-ALARCÓN,C Diclofenac fluorescein c-phycocyanin ORAC scavenging activity The scavenging activity of diclofenac was estimated by its capability to bleach ABTS radical cation, and ORAC (oxygen radical absorbance capacity) methodology. In addition, GC-MS technique was used to establish if the lactamic derivative of diclofenac was formed as final product in the interaction with peroxyl radicals. Diclofenac bleached ABTS radical cation in a concentration-dependent way. In ORAC assays, diclofenac inhibited the consumption of fluorescein and c-phycocyanin mediated by peroxyl radicals. An ORAC-fluorescein, and ORAC-c-phycocyanin value of 0.3, and 0.004 was estimated, respectively. From the downcurvature observed in Stern-Volmer-like plots, we postulated that the low protection of c-phycocyanin is related to secondary damage of c-phycocyanin by the secondary radical of diclofenac. By GC-MS studies only a 2,6-dichloroaniline derivative was detected as product afterthe reaction with peroxyl radicals.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.54 n.1 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000100014en10.4067/S0717-97072009000100014
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Diclofenac fluorescein c-phycocyanin ORAC scavenging activity
spellingShingle	Diclofenac fluorescein c-phycocyanin ORAC scavenging activity ROJO,C ÁLVAREZ-FIGUEROA,M. J SOTO,M CAÑETE,A PESSOA-MAHANA,D LÓPEZ-ALARCÓN,C SCAVENGING ACTIVITY OF DICLOFENAC: INTERACTION WITH ABTS RADICAL CATION AND PEROXYL RADICALS
description	The scavenging activity of diclofenac was estimated by its capability to bleach ABTS radical cation, and ORAC (oxygen radical absorbance capacity) methodology. In addition, GC-MS technique was used to establish if the lactamic derivative of diclofenac was formed as final product in the interaction with peroxyl radicals. Diclofenac bleached ABTS radical cation in a concentration-dependent way. In ORAC assays, diclofenac inhibited the consumption of fluorescein and c-phycocyanin mediated by peroxyl radicals. An ORAC-fluorescein, and ORAC-c-phycocyanin value of 0.3, and 0.004 was estimated, respectively. From the downcurvature observed in Stern-Volmer-like plots, we postulated that the low protection of c-phycocyanin is related to secondary damage of c-phycocyanin by the secondary radical of diclofenac. By GC-MS studies only a 2,6-dichloroaniline derivative was detected as product afterthe reaction with peroxyl radicals.
author	ROJO,C ÁLVAREZ-FIGUEROA,M. J SOTO,M CAÑETE,A PESSOA-MAHANA,D LÓPEZ-ALARCÓN,C
author_facet	ROJO,C ÁLVAREZ-FIGUEROA,M. J SOTO,M CAÑETE,A PESSOA-MAHANA,D LÓPEZ-ALARCÓN,C
author_sort	ROJO,C
title	SCAVENGING ACTIVITY OF DICLOFENAC: INTERACTION WITH ABTS RADICAL CATION AND PEROXYL RADICALS
title_short	SCAVENGING ACTIVITY OF DICLOFENAC: INTERACTION WITH ABTS RADICAL CATION AND PEROXYL RADICALS
title_full	SCAVENGING ACTIVITY OF DICLOFENAC: INTERACTION WITH ABTS RADICAL CATION AND PEROXYL RADICALS
title_fullStr	SCAVENGING ACTIVITY OF DICLOFENAC: INTERACTION WITH ABTS RADICAL CATION AND PEROXYL RADICALS
title_full_unstemmed	SCAVENGING ACTIVITY OF DICLOFENAC: INTERACTION WITH ABTS RADICAL CATION AND PEROXYL RADICALS
title_sort	scavenging activity of diclofenac: interaction with abts radical cation and peroxyl radicals
publisher	Sociedad Chilena de Química
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000100014
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SCAVENGING ACTIVITY OF DICLOFENAC: INTERACTION WITH ABTS RADICAL CATION AND PEROXYL RADICALS

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