SIMULTANEOUS DETERMINATION OF DIBUCAINE AND CHLORPHENAMINE MALEATE USING DIFFERENT MATHEMATICAL SPECTROPHOTOMETRIC APPROACHES

This paper analyses several mathematical approaches for simultaneous determination of dibucaine (D) and chlorphenamine maléate (CM). The different calibration methods utilised were: first derivative spectrophotometry (FDS), classical least squares (CLS), regression of partial least squares (PLS), an...

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Autores principales:	SOTO,CÉSAR, CONTRERAS,DAVID, TORAL,M. INÉS, BASAEZ,LUIS, FREER,JUANITA
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2009
Materias:	Spectrophotometry derivative CLS PCR PLS dibucaine chlorphenamine maléate
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000200003
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spelling	oai:scielo:S0717-970720090002000032009-10-07SIMULTANEOUS DETERMINATION OF DIBUCAINE AND CHLORPHENAMINE MALEATE USING DIFFERENT MATHEMATICAL SPECTROPHOTOMETRIC APPROACHESSOTO,CÉSARCONTRERAS,DAVIDTORAL,M. INÉSBASAEZ,LUISFREER,JUANITA Spectrophotometry derivative CLS PCR PLS dibucaine chlorphenamine maléate This paper analyses several mathematical approaches for simultaneous determination of dibucaine (D) and chlorphenamine maléate (CM). The different calibration methods utilised were: first derivative spectrophotometry (FDS), classical least squares (CLS), regression of partial least squares (PLS), and principal components regression (PCR). For the multivariated methods, the limits of detection (LOD) for multivariate calibrations were determined creating a surrogate signal variable (SSV). The recoveries were selected to confirm the validity of the models. In general, the CLS model presents the poorest recovery. In order to compare the exactitude of the different calibration methods, a one-way ANOVA test was performed. In these analyses, only the CLS calibration method produced a significantly different prediction. The lowest LODs were achieved by CLS and FDS, although the former method presented lower precision and poor prediction. Therefore, the FDS method is proposed as a pure binary calibration method for D-CM mixed samples, including the prepared according to a medical prescription and plasma samples simulating an event of intoxication.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.54 n.2 20092009-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000200003en10.4067/S0717-97072009000200003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Spectrophotometry derivative CLS PCR PLS dibucaine chlorphenamine maléate
spellingShingle	Spectrophotometry derivative CLS PCR PLS dibucaine chlorphenamine maléate SOTO,CÉSAR CONTRERAS,DAVID TORAL,M. INÉS BASAEZ,LUIS FREER,JUANITA SIMULTANEOUS DETERMINATION OF DIBUCAINE AND CHLORPHENAMINE MALEATE USING DIFFERENT MATHEMATICAL SPECTROPHOTOMETRIC APPROACHES
description	This paper analyses several mathematical approaches for simultaneous determination of dibucaine (D) and chlorphenamine maléate (CM). The different calibration methods utilised were: first derivative spectrophotometry (FDS), classical least squares (CLS), regression of partial least squares (PLS), and principal components regression (PCR). For the multivariated methods, the limits of detection (LOD) for multivariate calibrations were determined creating a surrogate signal variable (SSV). The recoveries were selected to confirm the validity of the models. In general, the CLS model presents the poorest recovery. In order to compare the exactitude of the different calibration methods, a one-way ANOVA test was performed. In these analyses, only the CLS calibration method produced a significantly different prediction. The lowest LODs were achieved by CLS and FDS, although the former method presented lower precision and poor prediction. Therefore, the FDS method is proposed as a pure binary calibration method for D-CM mixed samples, including the prepared according to a medical prescription and plasma samples simulating an event of intoxication.
author	SOTO,CÉSAR CONTRERAS,DAVID TORAL,M. INÉS BASAEZ,LUIS FREER,JUANITA
author_facet	SOTO,CÉSAR CONTRERAS,DAVID TORAL,M. INÉS BASAEZ,LUIS FREER,JUANITA
author_sort	SOTO,CÉSAR
title	SIMULTANEOUS DETERMINATION OF DIBUCAINE AND CHLORPHENAMINE MALEATE USING DIFFERENT MATHEMATICAL SPECTROPHOTOMETRIC APPROACHES
title_short	SIMULTANEOUS DETERMINATION OF DIBUCAINE AND CHLORPHENAMINE MALEATE USING DIFFERENT MATHEMATICAL SPECTROPHOTOMETRIC APPROACHES
title_full	SIMULTANEOUS DETERMINATION OF DIBUCAINE AND CHLORPHENAMINE MALEATE USING DIFFERENT MATHEMATICAL SPECTROPHOTOMETRIC APPROACHES
title_fullStr	SIMULTANEOUS DETERMINATION OF DIBUCAINE AND CHLORPHENAMINE MALEATE USING DIFFERENT MATHEMATICAL SPECTROPHOTOMETRIC APPROACHES
title_full_unstemmed	SIMULTANEOUS DETERMINATION OF DIBUCAINE AND CHLORPHENAMINE MALEATE USING DIFFERENT MATHEMATICAL SPECTROPHOTOMETRIC APPROACHES
title_sort	simultaneous determination of dibucaine and chlorphenamine maleate using different mathematical spectrophotometric approaches
publisher	Sociedad Chilena de Química
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000200003
work_keys_str_mv	AT sotocesar simultaneousdeterminationofdibucaineandchlorphenaminemaleateusingdifferentmathematicalspectrophotometricapproaches AT contrerasdavid simultaneousdeterminationofdibucaineandchlorphenaminemaleateusingdifferentmathematicalspectrophotometricapproaches AT toralmines simultaneousdeterminationofdibucaineandchlorphenaminemaleateusingdifferentmathematicalspectrophotometricapproaches AT basaezluis simultaneousdeterminationofdibucaineandchlorphenaminemaleateusingdifferentmathematicalspectrophotometricapproaches AT freerjuanita simultaneousdeterminationofdibucaineandchlorphenaminemaleateusingdifferentmathematicalspectrophotometricapproaches
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SIMULTANEOUS DETERMINATION OF DIBUCAINE AND CHLORPHENAMINE MALEATE USING DIFFERENT MATHEMATICAL SPECTROPHOTOMETRIC APPROACHES

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