SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF NORFLOXACIN, OFLOXACIN AND LOMEFLOXACIN IN RABBIT BLOOD SERUM BY USE OF CHEMOMETRICS

A spectrophotometric method for the simultaneous determination of norfloxacin, ofloxacin and lomefloxacin, is described. The spectrophotometric analysis is made possible with the aid of chemometric methods, such as classical least square (CLS), principal component regression (PCR), partial least squ...

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Autores principales:	HUANG,XI-GEN, ZHANG,HENG-SONG, LI,YAN-XIA, LI,MING-FANG
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2009
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000300001
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spelling	oai:scielo:S0717-970720090003000012010-03-17SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF NORFLOXACIN, OFLOXACIN AND LOMEFLOXACIN IN RABBIT BLOOD SERUM BY USE OF CHEMOMETRICSHUANG,XI-GENZHANG,HENG-SONGLI,YAN-XIALI,MING-FANGA spectrophotometric method for the simultaneous determination of norfloxacin, ofloxacin and lomefloxacin, is described. The spectrophotometric analysis is made possible with the aid of chemometric methods, such as classical least square (CLS), principal component regression (PCR), partial least square (PLS), and radial basis function-artificial neural network (RBF-ANN). Spectra were collected in the 190-400 nm range from a set of samples of three compounds in a Britton-Robinson buffer solution (pH=1.81). This approach overcomes the significant practical difficulties of severe spectral overlap. It was found that peak intensities are proportional to the concentration for each analyte. The obtained linear calibration concentration ranges are 0.6-13.8, 0.5-15.0, and 0.5-15.0 mg L-1 for norfloxacin, ofloxacin and lomefloxacin, respectively, and the LODs for the three analytes were found to be approximately 6-24x 10-2 mg L-1 . The proposed method was applied to a prediction set of synthetic mixtures of three analytes. The results were shown that the RBF-ANN model gave the best results (RPE T = 3.0%). This method was also applied for the determination of norfloxacin, ofloxacin and lomefloxacin in rabbit blood serum, and these compared well with those from the alternative analysis by HPLC.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.54 n.3 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000300001en10.4067/S0717-97072009000300001
institution	Scielo Chile
collection	Scielo Chile
language	English
description	A spectrophotometric method for the simultaneous determination of norfloxacin, ofloxacin and lomefloxacin, is described. The spectrophotometric analysis is made possible with the aid of chemometric methods, such as classical least square (CLS), principal component regression (PCR), partial least square (PLS), and radial basis function-artificial neural network (RBF-ANN). Spectra were collected in the 190-400 nm range from a set of samples of three compounds in a Britton-Robinson buffer solution (pH=1.81). This approach overcomes the significant practical difficulties of severe spectral overlap. It was found that peak intensities are proportional to the concentration for each analyte. The obtained linear calibration concentration ranges are 0.6-13.8, 0.5-15.0, and 0.5-15.0 mg L-1 for norfloxacin, ofloxacin and lomefloxacin, respectively, and the LODs for the three analytes were found to be approximately 6-24x 10-2 mg L-1 . The proposed method was applied to a prediction set of synthetic mixtures of three analytes. The results were shown that the RBF-ANN model gave the best results (RPE T = 3.0%). This method was also applied for the determination of norfloxacin, ofloxacin and lomefloxacin in rabbit blood serum, and these compared well with those from the alternative analysis by HPLC.
author	HUANG,XI-GEN ZHANG,HENG-SONG LI,YAN-XIA LI,MING-FANG
spellingShingle	HUANG,XI-GEN ZHANG,HENG-SONG LI,YAN-XIA LI,MING-FANG SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF NORFLOXACIN, OFLOXACIN AND LOMEFLOXACIN IN RABBIT BLOOD SERUM BY USE OF CHEMOMETRICS
author_facet	HUANG,XI-GEN ZHANG,HENG-SONG LI,YAN-XIA LI,MING-FANG
author_sort	HUANG,XI-GEN
title	SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF NORFLOXACIN, OFLOXACIN AND LOMEFLOXACIN IN RABBIT BLOOD SERUM BY USE OF CHEMOMETRICS
title_short	SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF NORFLOXACIN, OFLOXACIN AND LOMEFLOXACIN IN RABBIT BLOOD SERUM BY USE OF CHEMOMETRICS
title_full	SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF NORFLOXACIN, OFLOXACIN AND LOMEFLOXACIN IN RABBIT BLOOD SERUM BY USE OF CHEMOMETRICS
title_fullStr	SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF NORFLOXACIN, OFLOXACIN AND LOMEFLOXACIN IN RABBIT BLOOD SERUM BY USE OF CHEMOMETRICS
title_full_unstemmed	SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF NORFLOXACIN, OFLOXACIN AND LOMEFLOXACIN IN RABBIT BLOOD SERUM BY USE OF CHEMOMETRICS
title_sort	simultaneous spectrophotometric determination of norfloxacin, ofloxacin and lomefloxacin in rabbit blood serum by use of chemometrics
publisher	Sociedad Chilena de Química
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000300001
work_keys_str_mv	AT huangxigen simultaneousspectrophotometricdeterminationofnorfloxacinofloxacinandlomefloxacininrabbitbloodserumbyuseofchemometrics AT zhanghengsong simultaneousspectrophotometricdeterminationofnorfloxacinofloxacinandlomefloxacininrabbitbloodserumbyuseofchemometrics AT liyanxia simultaneousspectrophotometricdeterminationofnorfloxacinofloxacinandlomefloxacininrabbitbloodserumbyuseofchemometrics AT limingfang simultaneousspectrophotometricdeterminationofnorfloxacinofloxacinandlomefloxacininrabbitbloodserumbyuseofchemometrics
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SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF NORFLOXACIN, OFLOXACIN AND LOMEFLOXACIN IN RABBIT BLOOD SERUM BY USE OF CHEMOMETRICS

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