COMPARISON BETWEEN UV SPECTROPHOTOMETRY AND ANION EXCHANGE CHROMATOGRAPHY TECHNIQUES FOR QUANTIFICATION OF HEXENURONIC ACID (HEXA) IN UNBLEACHED KRAFT PULPS OF EUCALYPTUS GLOBULUS

Two different methodologies were optimized for hexenuronic acid (HexA) quantification in E. globulus kraft pulps. In one, pulps were hydrolyzed with mercuric chloride/sodium acetate and quantified by ultraviolet spectrophotometry (UV technique); in the other one, the hydrolysis was performed with su...

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Autores principales:	MONRROY,MARIEL, SANHUEZA,LORETO, TEIXEIRA MENDONÇA,REGIS, BAEZA,JAIME, FREER,JUANITA
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2009
Materias:	Eucalyptus globulus kraft pulping hexenuronic acids UV spectrophotometry anion exchange chromatography
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000300008
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spelling	oai:scielo:S0717-970720090003000082010-03-17COMPARISON BETWEEN UV SPECTROPHOTOMETRY AND ANION EXCHANGE CHROMATOGRAPHY TECHNIQUES FOR QUANTIFICATION OF HEXENURONIC ACID (HEXA) IN UNBLEACHED KRAFT PULPS OF EUCALYPTUS GLOBULUSMONRROY,MARIELSANHUEZA,LORETOTEIXEIRA MENDONÇA,REGISBAEZA,JAIMEFREER,JUANITA Eucalyptus globulus kraft pulping hexenuronic acids UV spectrophotometry anion exchange chromatography Two different methodologies were optimized for hexenuronic acid (HexA) quantification in E. globulus kraft pulps. In one, pulps were hydrolyzed with mercuric chloride/sodium acetate and quantified by ultraviolet spectrophotometry (UV technique); in the other one, the hydrolysis was performed with sulfuric acid and the degradation products of HexA quantified by anion exchange chromatography (AEC technique). In order to determine the optimal conditions for maximum remo val of HexA from pulps by acid hydrolysis, the variables time, pH, and temperature were studied. The influences of these three variables were determined by means of aresponse surface methodology (RSM). The optimized conditions for the hydrolysis by the UV technique were: 45 min, pH 5.7 and 80°C. While, for hydrolysis using the AEC technique, the optimized conditions were: 3.1 h, pH 0.7 and 97°C. Both AEC and UV methods presented good reproducibility with 2.1% and 1.4% relative standard deviation (RSD), respectively. However, HexA values measured by the AEC technique method were 11-30% lower than those measured using the UV technique. For additional information, five selected pulps were analyzed by other methods, known as KTH and HUT The results obtained by the four methods employed showed significant variations in the HexA quantification. The highest HexA values were obtained using the UV technique. The KTH, AEC and HUT methods provided HexA amounts in 7-16%, 17-24% and 24-30% lower than the values obtained by UV method, respectively, for the same set of pulps. The UV technique is more susceptible to be interfered with pulp hydrolysis by products, attributed mainly to lignin solubilization, while the AEC technique allows direct measurement of HexA hydrolysis products without interference from other compounds.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.54 n.3 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000300008en10.4067/S0717-97072009000300008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Eucalyptus globulus kraft pulping hexenuronic acids UV spectrophotometry anion exchange chromatography
spellingShingle	Eucalyptus globulus kraft pulping hexenuronic acids UV spectrophotometry anion exchange chromatography MONRROY,MARIEL SANHUEZA,LORETO TEIXEIRA MENDONÇA,REGIS BAEZA,JAIME FREER,JUANITA COMPARISON BETWEEN UV SPECTROPHOTOMETRY AND ANION EXCHANGE CHROMATOGRAPHY TECHNIQUES FOR QUANTIFICATION OF HEXENURONIC ACID (HEXA) IN UNBLEACHED KRAFT PULPS OF EUCALYPTUS GLOBULUS
description	Two different methodologies were optimized for hexenuronic acid (HexA) quantification in E. globulus kraft pulps. In one, pulps were hydrolyzed with mercuric chloride/sodium acetate and quantified by ultraviolet spectrophotometry (UV technique); in the other one, the hydrolysis was performed with sulfuric acid and the degradation products of HexA quantified by anion exchange chromatography (AEC technique). In order to determine the optimal conditions for maximum remo val of HexA from pulps by acid hydrolysis, the variables time, pH, and temperature were studied. The influences of these three variables were determined by means of aresponse surface methodology (RSM). The optimized conditions for the hydrolysis by the UV technique were: 45 min, pH 5.7 and 80°C. While, for hydrolysis using the AEC technique, the optimized conditions were: 3.1 h, pH 0.7 and 97°C. Both AEC and UV methods presented good reproducibility with 2.1% and 1.4% relative standard deviation (RSD), respectively. However, HexA values measured by the AEC technique method were 11-30% lower than those measured using the UV technique. For additional information, five selected pulps were analyzed by other methods, known as KTH and HUT The results obtained by the four methods employed showed significant variations in the HexA quantification. The highest HexA values were obtained using the UV technique. The KTH, AEC and HUT methods provided HexA amounts in 7-16%, 17-24% and 24-30% lower than the values obtained by UV method, respectively, for the same set of pulps. The UV technique is more susceptible to be interfered with pulp hydrolysis by products, attributed mainly to lignin solubilization, while the AEC technique allows direct measurement of HexA hydrolysis products without interference from other compounds.
author	MONRROY,MARIEL SANHUEZA,LORETO TEIXEIRA MENDONÇA,REGIS BAEZA,JAIME FREER,JUANITA
author_facet	MONRROY,MARIEL SANHUEZA,LORETO TEIXEIRA MENDONÇA,REGIS BAEZA,JAIME FREER,JUANITA
author_sort	MONRROY,MARIEL
title	COMPARISON BETWEEN UV SPECTROPHOTOMETRY AND ANION EXCHANGE CHROMATOGRAPHY TECHNIQUES FOR QUANTIFICATION OF HEXENURONIC ACID (HEXA) IN UNBLEACHED KRAFT PULPS OF EUCALYPTUS GLOBULUS
title_short	COMPARISON BETWEEN UV SPECTROPHOTOMETRY AND ANION EXCHANGE CHROMATOGRAPHY TECHNIQUES FOR QUANTIFICATION OF HEXENURONIC ACID (HEXA) IN UNBLEACHED KRAFT PULPS OF EUCALYPTUS GLOBULUS
title_full	COMPARISON BETWEEN UV SPECTROPHOTOMETRY AND ANION EXCHANGE CHROMATOGRAPHY TECHNIQUES FOR QUANTIFICATION OF HEXENURONIC ACID (HEXA) IN UNBLEACHED KRAFT PULPS OF EUCALYPTUS GLOBULUS
title_fullStr	COMPARISON BETWEEN UV SPECTROPHOTOMETRY AND ANION EXCHANGE CHROMATOGRAPHY TECHNIQUES FOR QUANTIFICATION OF HEXENURONIC ACID (HEXA) IN UNBLEACHED KRAFT PULPS OF EUCALYPTUS GLOBULUS
title_full_unstemmed	COMPARISON BETWEEN UV SPECTROPHOTOMETRY AND ANION EXCHANGE CHROMATOGRAPHY TECHNIQUES FOR QUANTIFICATION OF HEXENURONIC ACID (HEXA) IN UNBLEACHED KRAFT PULPS OF EUCALYPTUS GLOBULUS
title_sort	comparison between uv spectrophotometry and anion exchange chromatography techniques for quantification of hexenuronic acid (hexa) in unbleached kraft pulps of eucalyptus globulus
publisher	Sociedad Chilena de Química
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000300008
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_version_	1718445407252512768

COMPARISON BETWEEN UV SPECTROPHOTOMETRY AND ANION EXCHANGE CHROMATOGRAPHY TECHNIQUES FOR QUANTIFICATION OF HEXENURONIC ACID (HEXA) IN UNBLEACHED KRAFT PULPS OF EUCALYPTUS GLOBULUS

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