INCREASE OF TOTAL GLUTATHIONE AS A RESPONSE TO CD INDUCED STRESS IN A CHILEAN ENDEMIC BRYOPHYTES (THUIDIUM SP.)

Bryophytes collected from the IV Region of Chile, were treated with CdCl2 to stimulate the synthesis of phytochelants. After cadmium treatment, total peptides were extracted from the plants and were analyzed using HPLC to search for the presence of phytochelatin molecules. Chromatography results sho...

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Autores principales: LEINENWEBER,GENNY, STEGEN,SUSANA, DIAZ-PALMA,PAULA
Lenguaje:English
Publicado: Sociedad Chilena de Química 2009
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000400017
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spelling oai:scielo:S0717-970720090004000172010-04-15INCREASE OF TOTAL GLUTATHIONE AS A RESPONSE TO CD INDUCED STRESS IN A CHILEAN ENDEMIC BRYOPHYTES (THUIDIUM SP.)LEINENWEBER,GENNYSTEGEN,SUSANADIAZ-PALMA,PAULA Bryophytes glutathione chromatography oxidative stress Bryophytes collected from the IV Region of Chile, were treated with CdCl2 to stimulate the synthesis of phytochelants. After cadmium treatment, total peptides were extracted from the plants and were analyzed using HPLC to search for the presence of phytochelatin molecules. Chromatography results showed the absence of phytochelatin-like molecules in bryophytes. However, glutathione and a molecule suspected to be γ-glutamil-cistein were detected. The latter compound is knowed to be involved in the metabolic pathway of the gluthatione and phytochelatin synthesis. The results indicated the absence of detectable phytochelatins in bryophytes containing heavy metal as contaminants. The increase in glutathione during the experimental period may have been due to a response to oxidative stress. Thus this work suggests an important role of the glutathione in the detoxifcation mechanisms of bryophytes.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.54 n.4 20092009-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000400017en10.4067/S0717-97072009000400017
institution Scielo Chile
collection Scielo Chile
language English
topic Bryophytes
glutathione
chromatography
oxidative stress
spellingShingle Bryophytes
glutathione
chromatography
oxidative stress
LEINENWEBER,GENNY
STEGEN,SUSANA
DIAZ-PALMA,PAULA
INCREASE OF TOTAL GLUTATHIONE AS A RESPONSE TO CD INDUCED STRESS IN A CHILEAN ENDEMIC BRYOPHYTES (THUIDIUM SP.)
description Bryophytes collected from the IV Region of Chile, were treated with CdCl2 to stimulate the synthesis of phytochelants. After cadmium treatment, total peptides were extracted from the plants and were analyzed using HPLC to search for the presence of phytochelatin molecules. Chromatography results showed the absence of phytochelatin-like molecules in bryophytes. However, glutathione and a molecule suspected to be γ-glutamil-cistein were detected. The latter compound is knowed to be involved in the metabolic pathway of the gluthatione and phytochelatin synthesis. The results indicated the absence of detectable phytochelatins in bryophytes containing heavy metal as contaminants. The increase in glutathione during the experimental period may have been due to a response to oxidative stress. Thus this work suggests an important role of the glutathione in the detoxifcation mechanisms of bryophytes.
author LEINENWEBER,GENNY
STEGEN,SUSANA
DIAZ-PALMA,PAULA
author_facet LEINENWEBER,GENNY
STEGEN,SUSANA
DIAZ-PALMA,PAULA
author_sort LEINENWEBER,GENNY
title INCREASE OF TOTAL GLUTATHIONE AS A RESPONSE TO CD INDUCED STRESS IN A CHILEAN ENDEMIC BRYOPHYTES (THUIDIUM SP.)
title_short INCREASE OF TOTAL GLUTATHIONE AS A RESPONSE TO CD INDUCED STRESS IN A CHILEAN ENDEMIC BRYOPHYTES (THUIDIUM SP.)
title_full INCREASE OF TOTAL GLUTATHIONE AS A RESPONSE TO CD INDUCED STRESS IN A CHILEAN ENDEMIC BRYOPHYTES (THUIDIUM SP.)
title_fullStr INCREASE OF TOTAL GLUTATHIONE AS A RESPONSE TO CD INDUCED STRESS IN A CHILEAN ENDEMIC BRYOPHYTES (THUIDIUM SP.)
title_full_unstemmed INCREASE OF TOTAL GLUTATHIONE AS A RESPONSE TO CD INDUCED STRESS IN A CHILEAN ENDEMIC BRYOPHYTES (THUIDIUM SP.)
title_sort increase of total glutathione as a response to cd induced stress in a chilean endemic bryophytes (thuidium sp.)
publisher Sociedad Chilena de Química
publishDate 2009
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000400017
work_keys_str_mv AT leinenwebergenny increaseoftotalglutathioneasaresponsetocdinducedstressinachileanendemicbryophytesthuidiumsp
AT stegensusana increaseoftotalglutathioneasaresponsetocdinducedstressinachileanendemicbryophytesthuidiumsp
AT diazpalmapaula increaseoftotalglutathioneasaresponsetocdinducedstressinachileanendemicbryophytesthuidiumsp
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