SIMULTANEOUS DETERMINATION OF EMTRICITABINE AND TENOFOVIR BY AREA UNDER CURVE AND DUAL WAVELENGTH SPECTROPHOTOMETRIC METHOD

Two methods for the simultaneous determination of Emtricitabine and Tenofovir by spectroscopy have been developed. These two simple, accurate and precise methods include Area Under the Curve (AUC) method and Dual Wavelength Method. From a solvent effect studies and the spectral behaviours of Emtrici...

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Autores principales:	GHORPADE,SWAPNIL A, SALI,MONALI S, KATEGAONKAR,ATUL H, PATEL,DHAVAL M, CHOUDHARI,VISHNU P, KUCHEKAR,BHANUDAS S
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2010
Materias:	Spectroscopy Emtricitabine Tenofovir Area Under Curve Method Dual Wavelength Pharmaceutical Formulation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000100027
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spelling	oai:scielo:S0717-970720100001000272010-04-29SIMULTANEOUS DETERMINATION OF EMTRICITABINE AND TENOFOVIR BY AREA UNDER CURVE AND DUAL WAVELENGTH SPECTROPHOTOMETRIC METHODGHORPADE,SWAPNIL ASALI,MONALI SKATEGAONKAR,ATUL HPATEL,DHAVAL MCHOUDHARI,VISHNU PKUCHEKAR,BHANUDAS S Spectroscopy Emtricitabine Tenofovir Area Under Curve Method Dual Wavelength Pharmaceutical Formulation Two methods for the simultaneous determination of Emtricitabine and Tenofovir by spectroscopy have been developed. These two simple, accurate and precise methods include Area Under the Curve (AUC) method and Dual Wavelength Method. From a solvent effect studies and the spectral behaviours of Emtricitabine and Tenofovir, methanol was selected as solvent. Emtricitabine shows máximum absorbance at 281 nm and Tenofovir shows máximum absorbance at 259 nm. For the AUC method, the wavelength ranges between 242-248 nm and 269-275 nm were selected with reference to the absorbance curves plotted between the wavelengths of 200-400 nm. In the second method i.e. Dual method in which two wavelengths were selected for each drug in a way so that the difference in absorbance is zero for another drug. Emtricitabine shows equal absorbance at 230.696 nm and 250 nm, where the differences in absorbance were measured for the determination of Tenofovir. Similarly, differences in absorbances at 250 nm and 268.670 nm were measured for determination of Emtricitabine. These methods allows rapid analysis of two drug combination. The results of analysis were validated statistically and by recovery studies. This tablet containing both drugs was assayed using the methods developed, showing a good accuracy and precision.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.55 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000100027en10.4067/S0717-97072010000100027
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Spectroscopy Emtricitabine Tenofovir Area Under Curve Method Dual Wavelength Pharmaceutical Formulation
spellingShingle	Spectroscopy Emtricitabine Tenofovir Area Under Curve Method Dual Wavelength Pharmaceutical Formulation GHORPADE,SWAPNIL A SALI,MONALI S KATEGAONKAR,ATUL H PATEL,DHAVAL M CHOUDHARI,VISHNU P KUCHEKAR,BHANUDAS S SIMULTANEOUS DETERMINATION OF EMTRICITABINE AND TENOFOVIR BY AREA UNDER CURVE AND DUAL WAVELENGTH SPECTROPHOTOMETRIC METHOD
description	Two methods for the simultaneous determination of Emtricitabine and Tenofovir by spectroscopy have been developed. These two simple, accurate and precise methods include Area Under the Curve (AUC) method and Dual Wavelength Method. From a solvent effect studies and the spectral behaviours of Emtricitabine and Tenofovir, methanol was selected as solvent. Emtricitabine shows máximum absorbance at 281 nm and Tenofovir shows máximum absorbance at 259 nm. For the AUC method, the wavelength ranges between 242-248 nm and 269-275 nm were selected with reference to the absorbance curves plotted between the wavelengths of 200-400 nm. In the second method i.e. Dual method in which two wavelengths were selected for each drug in a way so that the difference in absorbance is zero for another drug. Emtricitabine shows equal absorbance at 230.696 nm and 250 nm, where the differences in absorbance were measured for the determination of Tenofovir. Similarly, differences in absorbances at 250 nm and 268.670 nm were measured for determination of Emtricitabine. These methods allows rapid analysis of two drug combination. The results of analysis were validated statistically and by recovery studies. This tablet containing both drugs was assayed using the methods developed, showing a good accuracy and precision.
author	GHORPADE,SWAPNIL A SALI,MONALI S KATEGAONKAR,ATUL H PATEL,DHAVAL M CHOUDHARI,VISHNU P KUCHEKAR,BHANUDAS S
author_facet	GHORPADE,SWAPNIL A SALI,MONALI S KATEGAONKAR,ATUL H PATEL,DHAVAL M CHOUDHARI,VISHNU P KUCHEKAR,BHANUDAS S
author_sort	GHORPADE,SWAPNIL A
title	SIMULTANEOUS DETERMINATION OF EMTRICITABINE AND TENOFOVIR BY AREA UNDER CURVE AND DUAL WAVELENGTH SPECTROPHOTOMETRIC METHOD
title_short	SIMULTANEOUS DETERMINATION OF EMTRICITABINE AND TENOFOVIR BY AREA UNDER CURVE AND DUAL WAVELENGTH SPECTROPHOTOMETRIC METHOD
title_full	SIMULTANEOUS DETERMINATION OF EMTRICITABINE AND TENOFOVIR BY AREA UNDER CURVE AND DUAL WAVELENGTH SPECTROPHOTOMETRIC METHOD
title_fullStr	SIMULTANEOUS DETERMINATION OF EMTRICITABINE AND TENOFOVIR BY AREA UNDER CURVE AND DUAL WAVELENGTH SPECTROPHOTOMETRIC METHOD
title_full_unstemmed	SIMULTANEOUS DETERMINATION OF EMTRICITABINE AND TENOFOVIR BY AREA UNDER CURVE AND DUAL WAVELENGTH SPECTROPHOTOMETRIC METHOD
title_sort	simultaneous determination of emtricitabine and tenofovir by area under curve and dual wavelength spectrophotometric method
publisher	Sociedad Chilena de Química
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000100027
work_keys_str_mv	AT ghorpadeswapnila simultaneousdeterminationofemtricitabineandtenofovirbyareaundercurveanddualwavelengthspectrophotometricmethod AT salimonalis simultaneousdeterminationofemtricitabineandtenofovirbyareaundercurveanddualwavelengthspectrophotometricmethod AT kategaonkaratulh simultaneousdeterminationofemtricitabineandtenofovirbyareaundercurveanddualwavelengthspectrophotometricmethod AT pateldhavalm simultaneousdeterminationofemtricitabineandtenofovirbyareaundercurveanddualwavelengthspectrophotometricmethod AT choudharivishnup simultaneousdeterminationofemtricitabineandtenofovirbyareaundercurveanddualwavelengthspectrophotometricmethod AT kuchekarbhanudass simultaneousdeterminationofemtricitabineandtenofovirbyareaundercurveanddualwavelengthspectrophotometricmethod
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SIMULTANEOUS DETERMINATION OF EMTRICITABINE AND TENOFOVIR BY AREA UNDER CURVE AND DUAL WAVELENGTH SPECTROPHOTOMETRIC METHOD

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