VALIDATED RP-HPLC METHOD FOR QUANTITATION OF GLIQUIDONE IN PHARMACEUTICAL FORMULATION AND HUMAN SERUM

1. A simple isocratic reversed phase high performance liquid chromatographic (HPLC) method using C18 column with ultraviolet detection at 225 nm has been described for quantitative determination of gliquidone in formulation and human serum. The system is operated at room temperature using a mobile p...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	ARAYNE,M. SAEED, SULTANA,NAJMA, MIRZA,AGHA ZEESHAN, SIDDIQUI,FARHAN AHMED
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2010
Materias:	Gliquidone methylparaben UV detection RP-HPLC dosage formulation method validation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000200002
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-97072010000200002
record_format	dspace
spelling	oai:scielo:S0717-970720100002000022010-09-06VALIDATED RP-HPLC METHOD FOR QUANTITATION OF GLIQUIDONE IN PHARMACEUTICAL FORMULATION AND HUMAN SERUMARAYNE,M. SAEEDSULTANA,NAJMAMIRZA,AGHA ZEESHANSIDDIQUI,FARHAN AHMED Gliquidone methylparaben UV detection RP-HPLC dosage formulation method validation 1. A simple isocratic reversed phase high performance liquid chromatographic (HPLC) method using C18 column with ultraviolet detection at 225 nm has been described for quantitative determination of gliquidone in formulation and human serum. The system is operated at room temperature using a mobile phase consisting of methanol: water (90:10) adjusted to pH 3.50 with phosphoric acid with a flow rate of 1 in Lmin-1. Methylparaben is successfully used as an internal standard. The assay is reproducible, linear (concentration range of 0.60-25 µmL-1) with correlation coefficient of 0.9999 and accurate (99.23 to 102.78%). The intra and inter run precision results are 1.14 and 1.32%. The LOD and LOQ values are 0.03 and 0.6 µmL-1, respectively There are no interfering peaks duetothe excipients present in the pharmaceutical tablet. Thus, the proposed method is simple and suitable for the analysis of active ingredient in tablet dosage form and human serum.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.55 n.2 20102010-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000200002en10.4067/S0717-97072010000200002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gliquidone methylparaben UV detection RP-HPLC dosage formulation method validation
spellingShingle	Gliquidone methylparaben UV detection RP-HPLC dosage formulation method validation ARAYNE,M. SAEED SULTANA,NAJMA MIRZA,AGHA ZEESHAN SIDDIQUI,FARHAN AHMED VALIDATED RP-HPLC METHOD FOR QUANTITATION OF GLIQUIDONE IN PHARMACEUTICAL FORMULATION AND HUMAN SERUM
description	1. A simple isocratic reversed phase high performance liquid chromatographic (HPLC) method using C18 column with ultraviolet detection at 225 nm has been described for quantitative determination of gliquidone in formulation and human serum. The system is operated at room temperature using a mobile phase consisting of methanol: water (90:10) adjusted to pH 3.50 with phosphoric acid with a flow rate of 1 in Lmin-1. Methylparaben is successfully used as an internal standard. The assay is reproducible, linear (concentration range of 0.60-25 µmL-1) with correlation coefficient of 0.9999 and accurate (99.23 to 102.78%). The intra and inter run precision results are 1.14 and 1.32%. The LOD and LOQ values are 0.03 and 0.6 µmL-1, respectively There are no interfering peaks duetothe excipients present in the pharmaceutical tablet. Thus, the proposed method is simple and suitable for the analysis of active ingredient in tablet dosage form and human serum.
author	ARAYNE,M. SAEED SULTANA,NAJMA MIRZA,AGHA ZEESHAN SIDDIQUI,FARHAN AHMED
author_facet	ARAYNE,M. SAEED SULTANA,NAJMA MIRZA,AGHA ZEESHAN SIDDIQUI,FARHAN AHMED
author_sort	ARAYNE,M. SAEED
title	VALIDATED RP-HPLC METHOD FOR QUANTITATION OF GLIQUIDONE IN PHARMACEUTICAL FORMULATION AND HUMAN SERUM
title_short	VALIDATED RP-HPLC METHOD FOR QUANTITATION OF GLIQUIDONE IN PHARMACEUTICAL FORMULATION AND HUMAN SERUM
title_full	VALIDATED RP-HPLC METHOD FOR QUANTITATION OF GLIQUIDONE IN PHARMACEUTICAL FORMULATION AND HUMAN SERUM
title_fullStr	VALIDATED RP-HPLC METHOD FOR QUANTITATION OF GLIQUIDONE IN PHARMACEUTICAL FORMULATION AND HUMAN SERUM
title_full_unstemmed	VALIDATED RP-HPLC METHOD FOR QUANTITATION OF GLIQUIDONE IN PHARMACEUTICAL FORMULATION AND HUMAN SERUM
title_sort	validated rp-hplc method for quantitation of gliquidone in pharmaceutical formulation and human serum
publisher	Sociedad Chilena de Química
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000200002
work_keys_str_mv	AT araynemsaeed validatedrphplcmethodforquantitationofgliquidoneinpharmaceuticalformulationandhumanserum AT sultananajma validatedrphplcmethodforquantitationofgliquidoneinpharmaceuticalformulationandhumanserum AT mirzaaghazeeshan validatedrphplcmethodforquantitationofgliquidoneinpharmaceuticalformulationandhumanserum AT siddiquifarhanahmed validatedrphplcmethodforquantitationofgliquidoneinpharmaceuticalformulationandhumanserum
_version_	1718445424412459008

VALIDATED RP-HPLC METHOD FOR QUANTITATION OF GLIQUIDONE IN PHARMACEUTICAL FORMULATION AND HUMAN SERUM

Ejemplares similares