SIMULTANEOUS DETERMINATION OF CEFTRIAXONE SODIUM AND STATIN DRUGS IN PHARMACEUTICAL FORMULATIONS AND HUMAN SERUM BY RP-HPLC
An accurate, sensitive and least time consuming reverse phase high performance liquid chromatographic (RP-HPLC) method for the estimation of ceftriaxone in the presence of statin drugs in formulation and human serum has been developed and validated. Chromatographic separation was conducted on prepac...
Guardado en:
| Autores principales: | , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Sociedad Chilena de Química
2010
|
| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000200010 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0717-97072010000200010 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0717-970720100002000102010-09-06SIMULTANEOUS DETERMINATION OF CEFTRIAXONE SODIUM AND STATIN DRUGS IN PHARMACEUTICAL FORMULATIONS AND HUMAN SERUM BY RP-HPLCSULTANA,NAJMAARAYNE,M. SAEEDSHAHZAD,WASEEM ceftriaxone statin's simvastatin rosuvastatin atorvastatin pravastatin and RP-HPLC An accurate, sensitive and least time consuming reverse phase high performance liquid chromatographic (RP-HPLC) method for the estimation of ceftriaxone in the presence of statin drugs in formulation and human serum has been developed and validated. Chromatographic separation was conducted on prepacked Purospher Star, C18 (5 mm, 250 x 4.6 mm) column at room temperature usingmethanol:water:acetonitrile (70:15:20 v/v/v) as amobile phase, pH adjusted at 2.8 with ortho-phosphoric acid and at a flow rate of 1.0 mL/minute, while UV detection was performed at 240 nm. The results obtained showed a good agreement with the declared content. The method shows good linearity in the range of 2.5-25 µg/mL with a correlation coefficient 0.9966 - 0.9998 (inter-and intra-day RSD < 2 %).The limit of detection and quantification for ceftriaxone and statins in pharmaceutical formulation and serum were inthe range 0.124 -1.006 µg/mL. Analytical recovery was >98.72 %. The proposed methodmay be used forthe quantitative analysis of commonly administered statin's i.e. simvastatin, rosuvastatin atorvastatin and pravastatin alone or in combination with ceftriaxone from raw materials, dosage formulations and in serum. The established HPLC method is rapid, accurate and selective, because of its sensitivity and reproducibility.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.55 n.2 20102010-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000200010en10.4067/S0717-97072010000200010 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
ceftriaxone statin's simvastatin rosuvastatin atorvastatin pravastatin and RP-HPLC |
| spellingShingle |
ceftriaxone statin's simvastatin rosuvastatin atorvastatin pravastatin and RP-HPLC SULTANA,NAJMA ARAYNE,M. SAEED SHAHZAD,WASEEM SIMULTANEOUS DETERMINATION OF CEFTRIAXONE SODIUM AND STATIN DRUGS IN PHARMACEUTICAL FORMULATIONS AND HUMAN SERUM BY RP-HPLC |
| description |
An accurate, sensitive and least time consuming reverse phase high performance liquid chromatographic (RP-HPLC) method for the estimation of ceftriaxone in the presence of statin drugs in formulation and human serum has been developed and validated. Chromatographic separation was conducted on prepacked Purospher Star, C18 (5 mm, 250 x 4.6 mm) column at room temperature usingmethanol:water:acetonitrile (70:15:20 v/v/v) as amobile phase, pH adjusted at 2.8 with ortho-phosphoric acid and at a flow rate of 1.0 mL/minute, while UV detection was performed at 240 nm. The results obtained showed a good agreement with the declared content. The method shows good linearity in the range of 2.5-25 µg/mL with a correlation coefficient 0.9966 - 0.9998 (inter-and intra-day RSD < 2 %).The limit of detection and quantification for ceftriaxone and statins in pharmaceutical formulation and serum were inthe range 0.124 -1.006 µg/mL. Analytical recovery was >98.72 %. The proposed methodmay be used forthe quantitative analysis of commonly administered statin's i.e. simvastatin, rosuvastatin atorvastatin and pravastatin alone or in combination with ceftriaxone from raw materials, dosage formulations and in serum. The established HPLC method is rapid, accurate and selective, because of its sensitivity and reproducibility. |
| author |
SULTANA,NAJMA ARAYNE,M. SAEED SHAHZAD,WASEEM |
| author_facet |
SULTANA,NAJMA ARAYNE,M. SAEED SHAHZAD,WASEEM |
| author_sort |
SULTANA,NAJMA |
| title |
SIMULTANEOUS DETERMINATION OF CEFTRIAXONE SODIUM AND STATIN DRUGS IN PHARMACEUTICAL FORMULATIONS AND HUMAN SERUM BY RP-HPLC |
| title_short |
SIMULTANEOUS DETERMINATION OF CEFTRIAXONE SODIUM AND STATIN DRUGS IN PHARMACEUTICAL FORMULATIONS AND HUMAN SERUM BY RP-HPLC |
| title_full |
SIMULTANEOUS DETERMINATION OF CEFTRIAXONE SODIUM AND STATIN DRUGS IN PHARMACEUTICAL FORMULATIONS AND HUMAN SERUM BY RP-HPLC |
| title_fullStr |
SIMULTANEOUS DETERMINATION OF CEFTRIAXONE SODIUM AND STATIN DRUGS IN PHARMACEUTICAL FORMULATIONS AND HUMAN SERUM BY RP-HPLC |
| title_full_unstemmed |
SIMULTANEOUS DETERMINATION OF CEFTRIAXONE SODIUM AND STATIN DRUGS IN PHARMACEUTICAL FORMULATIONS AND HUMAN SERUM BY RP-HPLC |
| title_sort |
simultaneous determination of ceftriaxone sodium and statin drugs in pharmaceutical formulations and human serum by rp-hplc |
| publisher |
Sociedad Chilena de Química |
| publishDate |
2010 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000200010 |
| work_keys_str_mv |
AT sultananajma simultaneousdeterminationofceftriaxonesodiumandstatindrugsinpharmaceuticalformulationsandhumanserumbyrphplc AT araynemsaeed simultaneousdeterminationofceftriaxonesodiumandstatindrugsinpharmaceuticalformulationsandhumanserumbyrphplc AT shahzadwaseem simultaneousdeterminationofceftriaxonesodiumandstatindrugsinpharmaceuticalformulationsandhumanserumbyrphplc |
| _version_ |
1718445425810210816 |