CHEMICAL STABILITY OF CEFTRIAXONE BY A VALIDATED STABILITY-INDICATING LIQUID CHROMATOGRAPHIC METHOD

The chemical stability of ceftriaxone for injection reconstituted with water was studied at different storage conditions by a validated stability-indicating ion-pair liquid chromatographic method. The study was performed on ceftriaxone for injection samples obtained from two pharmaceutical laborator...

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Autores principales:	DE DIEGO,M, GODOY,G, MENNICKENT,S
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2010
Materias:	Stability ion-pair liquid chromatography ceftriaxone
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000300013
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spelling	oai:scielo:S0717-970720100003000132011-01-25CHEMICAL STABILITY OF CEFTRIAXONE BY A VALIDATED STABILITY-INDICATING LIQUID CHROMATOGRAPHIC METHODDE DIEGO,MGODOY,GMENNICKENT,S Stability ion-pair liquid chromatography ceftriaxone The chemical stability of ceftriaxone for injection reconstituted with water was studied at different storage conditions by a validated stability-indicating ion-pair liquid chromatographic method. The study was performed on ceftriaxone for injection samples obtained from two pharmaceutical laboratories, at three different temperatures: 23 °C ± 2 °C, + 8 °C ± 1 °C and - 20 °C ± 0.5 °C, over 26 days, 55 days, and 76 days, respectively. Chromatographic separation was achieved on a RP-18 column, using a mobile phase consisting of 250 mL acetonitrile, 50 mL phosphate buffer (pH 7.4; 0.1M), 3.2 g tetrabutylammonium bromide made up to a volume of 1 L with water, at a flow rate of 1.0 mL min-1 and UV detection at 260 nm. The stability-indicating capability of the assay was estabhshed by forced degradation studies under hydrolysis, high temperature and photolysis. The developed method was specific, stability indicating, accurate and precise for ceftriaxone determination. At room temperature and at + 8 °C the degradation of ceftriaxone followed first-order kinetics and the samples were chemically stable for at least 4 and 41 days respectively. At - 20 °C the concentration of all samples remained higher than 90% of the original concentration. Degradation of ceftriaxone was higher at room temperature; therefore lower storage temperatures improved the chemical stability of ceftriaxone for injection in water.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.55 n.3 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000300013en10.4067/S0717-97072010000300013
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Stability ion-pair liquid chromatography ceftriaxone
spellingShingle	Stability ion-pair liquid chromatography ceftriaxone DE DIEGO,M GODOY,G MENNICKENT,S CHEMICAL STABILITY OF CEFTRIAXONE BY A VALIDATED STABILITY-INDICATING LIQUID CHROMATOGRAPHIC METHOD
description	The chemical stability of ceftriaxone for injection reconstituted with water was studied at different storage conditions by a validated stability-indicating ion-pair liquid chromatographic method. The study was performed on ceftriaxone for injection samples obtained from two pharmaceutical laboratories, at three different temperatures: 23 °C ± 2 °C, + 8 °C ± 1 °C and - 20 °C ± 0.5 °C, over 26 days, 55 days, and 76 days, respectively. Chromatographic separation was achieved on a RP-18 column, using a mobile phase consisting of 250 mL acetonitrile, 50 mL phosphate buffer (pH 7.4; 0.1M), 3.2 g tetrabutylammonium bromide made up to a volume of 1 L with water, at a flow rate of 1.0 mL min-1 and UV detection at 260 nm. The stability-indicating capability of the assay was estabhshed by forced degradation studies under hydrolysis, high temperature and photolysis. The developed method was specific, stability indicating, accurate and precise for ceftriaxone determination. At room temperature and at + 8 °C the degradation of ceftriaxone followed first-order kinetics and the samples were chemically stable for at least 4 and 41 days respectively. At - 20 °C the concentration of all samples remained higher than 90% of the original concentration. Degradation of ceftriaxone was higher at room temperature; therefore lower storage temperatures improved the chemical stability of ceftriaxone for injection in water.
author	DE DIEGO,M GODOY,G MENNICKENT,S
author_facet	DE DIEGO,M GODOY,G MENNICKENT,S
author_sort	DE DIEGO,M
title	CHEMICAL STABILITY OF CEFTRIAXONE BY A VALIDATED STABILITY-INDICATING LIQUID CHROMATOGRAPHIC METHOD
title_short	CHEMICAL STABILITY OF CEFTRIAXONE BY A VALIDATED STABILITY-INDICATING LIQUID CHROMATOGRAPHIC METHOD
title_full	CHEMICAL STABILITY OF CEFTRIAXONE BY A VALIDATED STABILITY-INDICATING LIQUID CHROMATOGRAPHIC METHOD
title_fullStr	CHEMICAL STABILITY OF CEFTRIAXONE BY A VALIDATED STABILITY-INDICATING LIQUID CHROMATOGRAPHIC METHOD
title_full_unstemmed	CHEMICAL STABILITY OF CEFTRIAXONE BY A VALIDATED STABILITY-INDICATING LIQUID CHROMATOGRAPHIC METHOD
title_sort	chemical stability of ceftriaxone by a validated stability-indicating liquid chromatographic method
publisher	Sociedad Chilena de Química
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000300013
work_keys_str_mv	AT dediegom chemicalstabilityofceftriaxonebyavalidatedstabilityindicatingliquidchromatographicmethod AT godoyg chemicalstabilityofceftriaxonebyavalidatedstabilityindicatingliquidchromatographicmethod AT mennickents chemicalstabilityofceftriaxonebyavalidatedstabilityindicatingliquidchromatographicmethod
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CHEMICAL STABILITY OF CEFTRIAXONE BY A VALIDATED STABILITY-INDICATING LIQUID CHROMATOGRAPHIC METHOD

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