DEVELOPMENT AND VALIDATION OF A GC-NPD/micro-ECD METHOD USING DUAL COLUMN FOR THE DETERMINATION OF BENZODIAZEPINE IN HUMAN WHOLE BLOOD AND PLASMA

Four benzodiazepines flunitrazepam, nitrazepam, clonazepam, and alprazolam, have been analyzed from biological matrices by dual column gas chromatography using a nitrogen-phosphorus detector (NPD) and a micro-electron capture detector (m-ECD). The recoveries of the four benzodiazepines spiked into h...

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Autores principales:	BRAVO,FELIPE, LOBOS,CLAUDIO, VENEGAS,KARINA, BENITES,JULIO
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2010
Materias:	Benzodiazepines gas chromatography nitrogen-phosphorus detector micro-electron capture detector
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000400009
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spelling	oai:scielo:S0717-970720100004000092011-05-25DEVELOPMENT AND VALIDATION OF A GC-NPD/micro-ECD METHOD USING DUAL COLUMN FOR THE DETERMINATION OF BENZODIAZEPINE IN HUMAN WHOLE BLOOD AND PLASMABRAVO,FELIPELOBOS,CLAUDIOVENEGAS,KARINABENITES,JULIO Benzodiazepines gas chromatography nitrogen-phosphorus detector micro-electron capture detector Four benzodiazepines flunitrazepam, nitrazepam, clonazepam, and alprazolam, have been analyzed from biological matrices by dual column gas chromatography using a nitrogen-phosphorus detector (NPD) and a micro-electron capture detector (m-ECD). The recoveries of the four benzodiazepines spiked into human whole blood and plasma were 88.8 - 97.9 and 90.7 - 97.5%, respectively. The regression equations for the four benzodiazepines showed excellent linearity. The detection limits (LODs) were 0.14 - 0.95 ng/mL for whole blood and 0.13 - 0.93 ng/mL for plasma. The method is simple and sensitive for the determination of benzodiazepines in human whole blood and plasma, and may be useful in forensic science practice.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.55 n.4 20102010-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000400009en10.4067/S0717-97072010000400009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Benzodiazepines gas chromatography nitrogen-phosphorus detector micro-electron capture detector
spellingShingle	Benzodiazepines gas chromatography nitrogen-phosphorus detector micro-electron capture detector BRAVO,FELIPE LOBOS,CLAUDIO VENEGAS,KARINA BENITES,JULIO DEVELOPMENT AND VALIDATION OF A GC-NPD/micro-ECD METHOD USING DUAL COLUMN FOR THE DETERMINATION OF BENZODIAZEPINE IN HUMAN WHOLE BLOOD AND PLASMA
description	Four benzodiazepines flunitrazepam, nitrazepam, clonazepam, and alprazolam, have been analyzed from biological matrices by dual column gas chromatography using a nitrogen-phosphorus detector (NPD) and a micro-electron capture detector (m-ECD). The recoveries of the four benzodiazepines spiked into human whole blood and plasma were 88.8 - 97.9 and 90.7 - 97.5%, respectively. The regression equations for the four benzodiazepines showed excellent linearity. The detection limits (LODs) were 0.14 - 0.95 ng/mL for whole blood and 0.13 - 0.93 ng/mL for plasma. The method is simple and sensitive for the determination of benzodiazepines in human whole blood and plasma, and may be useful in forensic science practice.
author	BRAVO,FELIPE LOBOS,CLAUDIO VENEGAS,KARINA BENITES,JULIO
author_facet	BRAVO,FELIPE LOBOS,CLAUDIO VENEGAS,KARINA BENITES,JULIO
author_sort	BRAVO,FELIPE
title	DEVELOPMENT AND VALIDATION OF A GC-NPD/micro-ECD METHOD USING DUAL COLUMN FOR THE DETERMINATION OF BENZODIAZEPINE IN HUMAN WHOLE BLOOD AND PLASMA
title_short	DEVELOPMENT AND VALIDATION OF A GC-NPD/micro-ECD METHOD USING DUAL COLUMN FOR THE DETERMINATION OF BENZODIAZEPINE IN HUMAN WHOLE BLOOD AND PLASMA
title_full	DEVELOPMENT AND VALIDATION OF A GC-NPD/micro-ECD METHOD USING DUAL COLUMN FOR THE DETERMINATION OF BENZODIAZEPINE IN HUMAN WHOLE BLOOD AND PLASMA
title_fullStr	DEVELOPMENT AND VALIDATION OF A GC-NPD/micro-ECD METHOD USING DUAL COLUMN FOR THE DETERMINATION OF BENZODIAZEPINE IN HUMAN WHOLE BLOOD AND PLASMA
title_full_unstemmed	DEVELOPMENT AND VALIDATION OF A GC-NPD/micro-ECD METHOD USING DUAL COLUMN FOR THE DETERMINATION OF BENZODIAZEPINE IN HUMAN WHOLE BLOOD AND PLASMA
title_sort	development and validation of a gc-npd/micro-ecd method using dual column for the determination of benzodiazepine in human whole blood and plasma
publisher	Sociedad Chilena de Química
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000400009
work_keys_str_mv	AT bravofelipe developmentandvalidationofagcnpdmicroecdmethodusingdualcolumnforthedeterminationofbenzodiazepineinhumanwholebloodandplasma AT lobosclaudio developmentandvalidationofagcnpdmicroecdmethodusingdualcolumnforthedeterminationofbenzodiazepineinhumanwholebloodandplasma AT venegaskarina developmentandvalidationofagcnpdmicroecdmethodusingdualcolumnforthedeterminationofbenzodiazepineinhumanwholebloodandplasma AT benitesjulio developmentandvalidationofagcnpdmicroecdmethodusingdualcolumnforthedeterminationofbenzodiazepineinhumanwholebloodandplasma
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DEVELOPMENT AND VALIDATION OF A GC-NPD/micro-ECD METHOD USING DUAL COLUMN FOR THE DETERMINATION OF BENZODIAZEPINE IN HUMAN WHOLE BLOOD AND PLASMA

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