DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF EZETIMIBE AND LOVASTATIN IN HUMAN PLASMA

In the present study conditions were optimized for the development and validation of a simple, sensitive and isocratic reverse phase high performance liquid chromatographic method for the simultaneous determination of ezetimibe and lovastatin in human plasma using gemfibrozil as internal standard. A...

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Autores principales:	ULLAH KHAN,ISLAM, KAUSAR,TAYYABA, ASHFAQ,MUHAMMAD, SHARIF,SHAHZAD
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2010
Materias:	HPLC plasma diode array detector gemfibrozil
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000400011
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spelling	oai:scielo:S0717-970720100004000112011-05-25DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF EZETIMIBE AND LOVASTATIN IN HUMAN PLASMAULLAH KHAN,ISLAMKAUSAR,TAYYABAASHFAQ,MUHAMMADSHARIF,SHAHZAD HPLC plasma diode array detector gemfibrozil In the present study conditions were optimized for the development and validation of a simple, sensitive and isocratic reverse phase high performance liquid chromatographic method for the simultaneous determination of ezetimibe and lovastatin in human plasma using gemfibrozil as internal standard. After a single step extraction with acetonitrile, satisfactory elution of both the analytes along with internal standard was achieved on a Merck C-18 column using a mixture of 0.1 M ammonium acetate pH 5.0 and acetonitrile in the ratio of (28:72, v/v) at a flow rate of 1.5 ml/min. The detection was carried out at a wavelength of 240 nm using a photodiode array detector. The method was linear in the concentration range of 0.2-25 \|xg/ml for ezetimibe and 0.4-50 \|xg/ml for lovastatin. The extraction recovery of analytes was greater than 85 % and within day and between day precision was also satisfactory. The method was validated by performing its linearity, recovery, precision, LOD/LOQ values and stability of solutions. The total eluting time for the analysis is less than twelve minutes. As the plasma peaks did not interfere with the peaks of analytes so the method can be used for the determination of ezetimibe and lovastatin in human plasma and may possibly be for the pharmacokinetic study.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.55 n.4 20102010-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000400011en10.4067/S0717-97072010000400011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	HPLC plasma diode array detector gemfibrozil
spellingShingle	HPLC plasma diode array detector gemfibrozil ULLAH KHAN,ISLAM KAUSAR,TAYYABA ASHFAQ,MUHAMMAD SHARIF,SHAHZAD DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF EZETIMIBE AND LOVASTATIN IN HUMAN PLASMA
description	In the present study conditions were optimized for the development and validation of a simple, sensitive and isocratic reverse phase high performance liquid chromatographic method for the simultaneous determination of ezetimibe and lovastatin in human plasma using gemfibrozil as internal standard. After a single step extraction with acetonitrile, satisfactory elution of both the analytes along with internal standard was achieved on a Merck C-18 column using a mixture of 0.1 M ammonium acetate pH 5.0 and acetonitrile in the ratio of (28:72, v/v) at a flow rate of 1.5 ml/min. The detection was carried out at a wavelength of 240 nm using a photodiode array detector. The method was linear in the concentration range of 0.2-25 \|xg/ml for ezetimibe and 0.4-50 \|xg/ml for lovastatin. The extraction recovery of analytes was greater than 85 % and within day and between day precision was also satisfactory. The method was validated by performing its linearity, recovery, precision, LOD/LOQ values and stability of solutions. The total eluting time for the analysis is less than twelve minutes. As the plasma peaks did not interfere with the peaks of analytes so the method can be used for the determination of ezetimibe and lovastatin in human plasma and may possibly be for the pharmacokinetic study.
author	ULLAH KHAN,ISLAM KAUSAR,TAYYABA ASHFAQ,MUHAMMAD SHARIF,SHAHZAD
author_facet	ULLAH KHAN,ISLAM KAUSAR,TAYYABA ASHFAQ,MUHAMMAD SHARIF,SHAHZAD
author_sort	ULLAH KHAN,ISLAM
title	DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF EZETIMIBE AND LOVASTATIN IN HUMAN PLASMA
title_short	DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF EZETIMIBE AND LOVASTATIN IN HUMAN PLASMA
title_full	DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF EZETIMIBE AND LOVASTATIN IN HUMAN PLASMA
title_fullStr	DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF EZETIMIBE AND LOVASTATIN IN HUMAN PLASMA
title_full_unstemmed	DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF EZETIMIBE AND LOVASTATIN IN HUMAN PLASMA
title_sort	development and validation of liquid chromatographic method for the simultaneous estimation of ezetimibe and lovastatin in human plasma
publisher	Sociedad Chilena de Química
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000400011
work_keys_str_mv	AT ullahkhanislam developmentandvalidationofliquidchromatographicmethodforthesimultaneousestimationofezetimibeandlovastatininhumanplasma AT kausartayyaba developmentandvalidationofliquidchromatographicmethodforthesimultaneousestimationofezetimibeandlovastatininhumanplasma AT ashfaqmuhammad developmentandvalidationofliquidchromatographicmethodforthesimultaneousestimationofezetimibeandlovastatininhumanplasma AT sharifshahzad developmentandvalidationofliquidchromatographicmethodforthesimultaneousestimationofezetimibeandlovastatininhumanplasma
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DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF EZETIMIBE AND LOVASTATIN IN HUMAN PLASMA

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