SIMULTANEOUS DETERMINATION OF PHENYLEPHRINE HYDROCHLORIDE AND TROPICAMIDE IN OPHTHALMIC DOSAGE FORM WITH THREE RAPID DERIVATIVE SPECTROPHOTOMETRIC METHODS

Three spectrophotometric methods have been developed for simultaneous estimation of Phenylephrine hydrochloride (PHE) and Tropicamide (TPC) in their combined dosage form. Method-I: zero-crossing derivative spectrophotometry involves amplitudes measurement of the first derivative spectra of the binar...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	SARDANA,SWATI, MASHRU,R.C
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2010
Materias:	Phenylephrine Hydrochloride(PHE) Tropicamide(TPC) zero-crossing derivative spectrophotometry dual wavelength Ratio spectra derivative spectrophotometry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000400023
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-97072010000400023
record_format	dspace
spelling	oai:scielo:S0717-970720100004000232011-05-25SIMULTANEOUS DETERMINATION OF PHENYLEPHRINE HYDROCHLORIDE AND TROPICAMIDE IN OPHTHALMIC DOSAGE FORM WITH THREE RAPID DERIVATIVE SPECTROPHOTOMETRIC METHODSSARDANA,SWATIMASHRU,R.C Phenylephrine Hydrochloride(PHE) Tropicamide(TPC) zero-crossing derivative spectrophotometry dual wavelength Ratio spectra derivative spectrophotometry Three spectrophotometric methods have been developed for simultaneous estimation of Phenylephrine hydrochloride (PHE) and Tropicamide (TPC) in their combined dosage form. Method-I: zero-crossing derivative spectrophotometry involves amplitudes measurement of the first derivative spectra of the binary mixture at 284.0nm for PHE and 241.2nm for TPC. Method-II: dual wavelength,which uses the difference in absorbance at 260.8 nm and 268.2 nm for estimation of PHE and 245.4nm and 271.8nm for TPC. Method III: Ratio spectra derivative spectrophotometry, in which division of the absorption spectrum of the binary mixture by a standard spectrum of one of the components and then calculating first derivative amplitudes at 270.8nm for PHE and 240.8nm for TPC . The calibration graph follows Beer's law in the range of 25-125 mg/ml for PHE and 4-20 mg/ml for TPC for all the three methods. The accuracy and precision of the methods were validated statistically.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.55 n.4 20102010-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000400023en10.4067/S0717-97072010000400023
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Phenylephrine Hydrochloride(PHE) Tropicamide(TPC) zero-crossing derivative spectrophotometry dual wavelength Ratio spectra derivative spectrophotometry
spellingShingle	Phenylephrine Hydrochloride(PHE) Tropicamide(TPC) zero-crossing derivative spectrophotometry dual wavelength Ratio spectra derivative spectrophotometry SARDANA,SWATI MASHRU,R.C SIMULTANEOUS DETERMINATION OF PHENYLEPHRINE HYDROCHLORIDE AND TROPICAMIDE IN OPHTHALMIC DOSAGE FORM WITH THREE RAPID DERIVATIVE SPECTROPHOTOMETRIC METHODS
description	Three spectrophotometric methods have been developed for simultaneous estimation of Phenylephrine hydrochloride (PHE) and Tropicamide (TPC) in their combined dosage form. Method-I: zero-crossing derivative spectrophotometry involves amplitudes measurement of the first derivative spectra of the binary mixture at 284.0nm for PHE and 241.2nm for TPC. Method-II: dual wavelength,which uses the difference in absorbance at 260.8 nm and 268.2 nm for estimation of PHE and 245.4nm and 271.8nm for TPC. Method III: Ratio spectra derivative spectrophotometry, in which division of the absorption spectrum of the binary mixture by a standard spectrum of one of the components and then calculating first derivative amplitudes at 270.8nm for PHE and 240.8nm for TPC . The calibration graph follows Beer's law in the range of 25-125 mg/ml for PHE and 4-20 mg/ml for TPC for all the three methods. The accuracy and precision of the methods were validated statistically.
author	SARDANA,SWATI MASHRU,R.C
author_facet	SARDANA,SWATI MASHRU,R.C
author_sort	SARDANA,SWATI
title	SIMULTANEOUS DETERMINATION OF PHENYLEPHRINE HYDROCHLORIDE AND TROPICAMIDE IN OPHTHALMIC DOSAGE FORM WITH THREE RAPID DERIVATIVE SPECTROPHOTOMETRIC METHODS
title_short	SIMULTANEOUS DETERMINATION OF PHENYLEPHRINE HYDROCHLORIDE AND TROPICAMIDE IN OPHTHALMIC DOSAGE FORM WITH THREE RAPID DERIVATIVE SPECTROPHOTOMETRIC METHODS
title_full	SIMULTANEOUS DETERMINATION OF PHENYLEPHRINE HYDROCHLORIDE AND TROPICAMIDE IN OPHTHALMIC DOSAGE FORM WITH THREE RAPID DERIVATIVE SPECTROPHOTOMETRIC METHODS
title_fullStr	SIMULTANEOUS DETERMINATION OF PHENYLEPHRINE HYDROCHLORIDE AND TROPICAMIDE IN OPHTHALMIC DOSAGE FORM WITH THREE RAPID DERIVATIVE SPECTROPHOTOMETRIC METHODS
title_full_unstemmed	SIMULTANEOUS DETERMINATION OF PHENYLEPHRINE HYDROCHLORIDE AND TROPICAMIDE IN OPHTHALMIC DOSAGE FORM WITH THREE RAPID DERIVATIVE SPECTROPHOTOMETRIC METHODS
title_sort	simultaneous determination of phenylephrine hydrochloride and tropicamide in ophthalmic dosage form with three rapid derivative spectrophotometric methods
publisher	Sociedad Chilena de Química
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000400023
work_keys_str_mv	AT sardanaswati simultaneousdeterminationofphenylephrinehydrochlorideandtropicamideinophthalmicdosageformwiththreerapidderivativespectrophotometricmethods AT mashrurc simultaneousdeterminationofphenylephrinehydrochlorideandtropicamideinophthalmicdosageformwiththreerapidderivativespectrophotometricmethods
_version_	1718445439531876352

SIMULTANEOUS DETERMINATION OF PHENYLEPHRINE HYDROCHLORIDE AND TROPICAMIDE IN OPHTHALMIC DOSAGE FORM WITH THREE RAPID DERIVATIVE SPECTROPHOTOMETRIC METHODS

Ejemplares similares