VALIDATION OF A LIQUID CHROMATOGRAPHY-FLUORESCENCE SPECTROMETRIC METHOD FOR THE QUANTIFICATION OF FOUR QUINOLONES IN PIG AND CHICKEN MUSCLE FOLLOWING THE EUROPEAN UNION GUIDELINE EC/657/2002

Quinolones are a group of structurally related antibacterial compounds. Over the last years their use in animal production has been greatly increased. This increase can cause the resistance of bacteria. In order to protect human health the European Union (EU), as well as other countries, has establi...

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Autores principales:	VASQUEZ-MARTINEZ,Y, VILLEGAS R,L, VILLA,A, MASSIFF,G
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2011
Materias:	Quinolones pig and chicken muscle HPLC-fluorescence detection
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000100007
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spelling	oai:scielo:S0717-970720110001000072011-06-17VALIDATION OF A LIQUID CHROMATOGRAPHY-FLUORESCENCE SPECTROMETRIC METHOD FOR THE QUANTIFICATION OF FOUR QUINOLONES IN PIG AND CHICKEN MUSCLE FOLLOWING THE EUROPEAN UNION GUIDELINE EC/657/2002VASQUEZ-MARTINEZ,YVILLEGAS R,LVILLA,AMASSIFF,G Quinolones pig and chicken muscle HPLC-fluorescence detection Quinolones are a group of structurally related antibacterial compounds. Over the last years their use in animal production has been greatly increased. This increase can cause the resistance of bacteria. In order to protect human health the European Union (EU), as well as other countries, has established Maximum Residue Limits (MRL) for these antibacterial agents in several foods. Among these foods, pig and chicken muscle have different MRLs in the sub-ppm range. A liquid chromatography-fluorescence spectrometric method has been developed and validated for the simultaneous assessment of four of the most used quinolones in edible animal matrices production. Simultaneous quantification of enrofloxacin, ciprofloxacin, oxolinic acid and flumequine was achieved by a method involving an initial aqueous extraction step followed by a solid-liquid-phase extraction using reverse-phase columns (C-18) and a final injection of all quinolones in a single chromatographic run using HPLC-Fluorescence detection. Nalidixic acid was used as internal standard. In addition to the description of the analytical method developed for the quantification of the studied quinolones, the different validation parameters of this method according to Commission Decision EC /657/2002 of the EU are presented and discussed.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.56 n.1 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000100007en10.4067/S0717-97072011000100007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Quinolones pig and chicken muscle HPLC-fluorescence detection
spellingShingle	Quinolones pig and chicken muscle HPLC-fluorescence detection VASQUEZ-MARTINEZ,Y VILLEGAS R,L VILLA,A MASSIFF,G VALIDATION OF A LIQUID CHROMATOGRAPHY-FLUORESCENCE SPECTROMETRIC METHOD FOR THE QUANTIFICATION OF FOUR QUINOLONES IN PIG AND CHICKEN MUSCLE FOLLOWING THE EUROPEAN UNION GUIDELINE EC/657/2002
description	Quinolones are a group of structurally related antibacterial compounds. Over the last years their use in animal production has been greatly increased. This increase can cause the resistance of bacteria. In order to protect human health the European Union (EU), as well as other countries, has established Maximum Residue Limits (MRL) for these antibacterial agents in several foods. Among these foods, pig and chicken muscle have different MRLs in the sub-ppm range. A liquid chromatography-fluorescence spectrometric method has been developed and validated for the simultaneous assessment of four of the most used quinolones in edible animal matrices production. Simultaneous quantification of enrofloxacin, ciprofloxacin, oxolinic acid and flumequine was achieved by a method involving an initial aqueous extraction step followed by a solid-liquid-phase extraction using reverse-phase columns (C-18) and a final injection of all quinolones in a single chromatographic run using HPLC-Fluorescence detection. Nalidixic acid was used as internal standard. In addition to the description of the analytical method developed for the quantification of the studied quinolones, the different validation parameters of this method according to Commission Decision EC /657/2002 of the EU are presented and discussed.
author	VASQUEZ-MARTINEZ,Y VILLEGAS R,L VILLA,A MASSIFF,G
author_facet	VASQUEZ-MARTINEZ,Y VILLEGAS R,L VILLA,A MASSIFF,G
author_sort	VASQUEZ-MARTINEZ,Y
title	VALIDATION OF A LIQUID CHROMATOGRAPHY-FLUORESCENCE SPECTROMETRIC METHOD FOR THE QUANTIFICATION OF FOUR QUINOLONES IN PIG AND CHICKEN MUSCLE FOLLOWING THE EUROPEAN UNION GUIDELINE EC/657/2002
title_short	VALIDATION OF A LIQUID CHROMATOGRAPHY-FLUORESCENCE SPECTROMETRIC METHOD FOR THE QUANTIFICATION OF FOUR QUINOLONES IN PIG AND CHICKEN MUSCLE FOLLOWING THE EUROPEAN UNION GUIDELINE EC/657/2002
title_full	VALIDATION OF A LIQUID CHROMATOGRAPHY-FLUORESCENCE SPECTROMETRIC METHOD FOR THE QUANTIFICATION OF FOUR QUINOLONES IN PIG AND CHICKEN MUSCLE FOLLOWING THE EUROPEAN UNION GUIDELINE EC/657/2002
title_fullStr	VALIDATION OF A LIQUID CHROMATOGRAPHY-FLUORESCENCE SPECTROMETRIC METHOD FOR THE QUANTIFICATION OF FOUR QUINOLONES IN PIG AND CHICKEN MUSCLE FOLLOWING THE EUROPEAN UNION GUIDELINE EC/657/2002
title_full_unstemmed	VALIDATION OF A LIQUID CHROMATOGRAPHY-FLUORESCENCE SPECTROMETRIC METHOD FOR THE QUANTIFICATION OF FOUR QUINOLONES IN PIG AND CHICKEN MUSCLE FOLLOWING THE EUROPEAN UNION GUIDELINE EC/657/2002
title_sort	validation of a liquid chromatography-fluorescence spectrometric method for the quantification of four quinolones in pig and chicken muscle following the european union guideline ec/657/2002
publisher	Sociedad Chilena de Química
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000100007
work_keys_str_mv	AT vasquezmartinezy validationofaliquidchromatographyfluorescencespectrometricmethodforthequantificationoffourquinolonesinpigandchickenmusclefollowingtheeuropeanunionguidelineec6572002 AT villegasrl validationofaliquidchromatographyfluorescencespectrometricmethodforthequantificationoffourquinolonesinpigandchickenmusclefollowingtheeuropeanunionguidelineec6572002 AT villaa validationofaliquidchromatographyfluorescencespectrometricmethodforthequantificationoffourquinolonesinpigandchickenmusclefollowingtheeuropeanunionguidelineec6572002 AT massiffg validationofaliquidchromatographyfluorescencespectrometricmethodforthequantificationoffourquinolonesinpigandchickenmusclefollowingtheeuropeanunionguidelineec6572002
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VALIDATION OF A LIQUID CHROMATOGRAPHY-FLUORESCENCE SPECTROMETRIC METHOD FOR THE QUANTIFICATION OF FOUR QUINOLONES IN PIG AND CHICKEN MUSCLE FOLLOWING THE EUROPEAN UNION GUIDELINE EC/657/2002

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