PHARMACOKINETIC STUDY OF RISPERIDONE: APPLICATION OF A HPLC METHOD WITH SOLID PHASE EXTRACTION

A new, simplified solid phase extraction procedure for the determination of risperidone and 9-hydroxyrisperidone in human plasma has been developed. This method involves the use of an optimized extraction protocol developed in Waters OASIS® HLB 30mg 1cc extraction columns using 1 mL of human serum....

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Autores principales:	TORRES V,PABLO, SEPÚLVEDA C,M. JACQUELINE, VON PLESSING R,CARLOS
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2011
Materias:	Risperidone 9-hydroxyrisperidone solid phase extraction
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000100019
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spelling	oai:scielo:S0717-970720110001000192011-06-17PHARMACOKINETIC STUDY OF RISPERIDONE: APPLICATION OF A HPLC METHOD WITH SOLID PHASE EXTRACTIONTORRES V,PABLOSEPÚLVEDA C,M. JACQUELINEVON PLESSING R,CARLOS Risperidone 9-hydroxyrisperidone solid phase extraction A new, simplified solid phase extraction procedure for the determination of risperidone and 9-hydroxyrisperidone in human plasma has been developed. This method involves the use of an optimized extraction protocol developed in Waters OASIS® HLB 30mg 1cc extraction columns using 1 mL of human serum. Separation was performed by HPLC using a Waters XTerra RP-18 (5 µm, 150x4,6 mm) column with a mobile phase consisting in acetonitrile - potassium dihydrogen phosphate 50 mM pH 3.4 (27/73). UV detection at 278 nm was used to quantify analytes, encountering good linearity (r² > 0.999) in the 2-100 ng/mL concentration range. The mean recovery was 92.4 % and 92.8 % for risperidone and 9-hydroxyrisperidone respectively, with an intraday - interday precision below 7%, and accuracy below 115 %. The method has been successfully applied in pharmacokinetic studies that require a large sample number.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.56 n.1 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000100019en10.4067/S0717-97072011000100019
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Risperidone 9-hydroxyrisperidone solid phase extraction
spellingShingle	Risperidone 9-hydroxyrisperidone solid phase extraction TORRES V,PABLO SEPÚLVEDA C,M. JACQUELINE VON PLESSING R,CARLOS PHARMACOKINETIC STUDY OF RISPERIDONE: APPLICATION OF A HPLC METHOD WITH SOLID PHASE EXTRACTION
description	A new, simplified solid phase extraction procedure for the determination of risperidone and 9-hydroxyrisperidone in human plasma has been developed. This method involves the use of an optimized extraction protocol developed in Waters OASIS® HLB 30mg 1cc extraction columns using 1 mL of human serum. Separation was performed by HPLC using a Waters XTerra RP-18 (5 µm, 150x4,6 mm) column with a mobile phase consisting in acetonitrile - potassium dihydrogen phosphate 50 mM pH 3.4 (27/73). UV detection at 278 nm was used to quantify analytes, encountering good linearity (r² > 0.999) in the 2-100 ng/mL concentration range. The mean recovery was 92.4 % and 92.8 % for risperidone and 9-hydroxyrisperidone respectively, with an intraday - interday precision below 7%, and accuracy below 115 %. The method has been successfully applied in pharmacokinetic studies that require a large sample number.
author	TORRES V,PABLO SEPÚLVEDA C,M. JACQUELINE VON PLESSING R,CARLOS
author_facet	TORRES V,PABLO SEPÚLVEDA C,M. JACQUELINE VON PLESSING R,CARLOS
author_sort	TORRES V,PABLO
title	PHARMACOKINETIC STUDY OF RISPERIDONE: APPLICATION OF A HPLC METHOD WITH SOLID PHASE EXTRACTION
title_short	PHARMACOKINETIC STUDY OF RISPERIDONE: APPLICATION OF A HPLC METHOD WITH SOLID PHASE EXTRACTION
title_full	PHARMACOKINETIC STUDY OF RISPERIDONE: APPLICATION OF A HPLC METHOD WITH SOLID PHASE EXTRACTION
title_fullStr	PHARMACOKINETIC STUDY OF RISPERIDONE: APPLICATION OF A HPLC METHOD WITH SOLID PHASE EXTRACTION
title_full_unstemmed	PHARMACOKINETIC STUDY OF RISPERIDONE: APPLICATION OF A HPLC METHOD WITH SOLID PHASE EXTRACTION
title_sort	pharmacokinetic study of risperidone: application of a hplc method with solid phase extraction
publisher	Sociedad Chilena de Química
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000100019
work_keys_str_mv	AT torresvpablo pharmacokineticstudyofrisperidoneapplicationofahplcmethodwithsolidphaseextraction AT sepulvedacmjacqueline pharmacokineticstudyofrisperidoneapplicationofahplcmethodwithsolidphaseextraction AT vonplessingrcarlos pharmacokineticstudyofrisperidoneapplicationofahplcmethodwithsolidphaseextraction
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PHARMACOKINETIC STUDY OF RISPERIDONE: APPLICATION OF A HPLC METHOD WITH SOLID PHASE EXTRACTION

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