APPLICATION OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF LAMIVUDINE AND TENOFOVIR DISOPROXIL FUMARATE IN PHARMACEUTICAL DOSAGE FORM

A simple, precise and accurate high performance thin layer chromatographic method has been developed for the simultaneous determination of lamivudine (LAM) and tenofovir disoproxil fumarate (TDF) in pharmaceutical dosage form. The separation was carried out on Merck HPTLC aluminum plates of silica g...

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Autores principales:	CHANDRA,P, RATHORE,A. S, SATHIYANARAYANAN,L, MAHADIK,K. R
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2011
Materias:	lamivudine tenofovir disoproxil fumarate HPTLC validation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000200017
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spelling	oai:scielo:S0717-970720110002000172011-09-01APPLICATION OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF LAMIVUDINE AND TENOFOVIR DISOPROXIL FUMARATE IN PHARMACEUTICAL DOSAGE FORMCHANDRA,PRATHORE,A. SSATHIYANARAYANAN,LMAHADIK,K. R lamivudine tenofovir disoproxil fumarate HPTLC validation A simple, precise and accurate high performance thin layer chromatographic method has been developed for the simultaneous determination of lamivudine (LAM) and tenofovir disoproxil fumarate (TDF) in pharmaceutical dosage form. The separation was carried out on Merck HPTLC aluminum plates of silica gel 60 F254,(20 x 10 cm) with 250 µm thickness using chloroform: methanol: toluene (8: 2: 2, v/v/v) as mobile phase. HPTLC separation of the two drugs followed by densitometric measurement was carried out in the absorbance mode at 265 nm. The drugs were satisfactorily resolved with Rf values of 0.27± 0.01 and 0.51± 0.01 for LAM and TDF, respectively. The linear regression analysis data for the calibration plots showed good linear relationship with r²=0.9999 and 0.9996 for LAM and TDF, respectively in the concentration range of 60-210 ng spot¹ for each drug. The method was validated for precision, robustness, specificity and accuracy The limit of detection and quantitation were 20 and 40 ng spot¹, respectively for LAM and 30 and 60 ng spot¹, respectively for TDF. The proposed developed HPTLC method can be applied for identification and quantitative determination of LAM and TDF in bulk drug and pharmaceutical dosage form.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.56 n.2 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000200017en10.4067/S0717-97072011000200017
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	lamivudine tenofovir disoproxil fumarate HPTLC validation
spellingShingle	lamivudine tenofovir disoproxil fumarate HPTLC validation CHANDRA,P RATHORE,A. S SATHIYANARAYANAN,L MAHADIK,K. R APPLICATION OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF LAMIVUDINE AND TENOFOVIR DISOPROXIL FUMARATE IN PHARMACEUTICAL DOSAGE FORM
description	A simple, precise and accurate high performance thin layer chromatographic method has been developed for the simultaneous determination of lamivudine (LAM) and tenofovir disoproxil fumarate (TDF) in pharmaceutical dosage form. The separation was carried out on Merck HPTLC aluminum plates of silica gel 60 F254,(20 x 10 cm) with 250 µm thickness using chloroform: methanol: toluene (8: 2: 2, v/v/v) as mobile phase. HPTLC separation of the two drugs followed by densitometric measurement was carried out in the absorbance mode at 265 nm. The drugs were satisfactorily resolved with Rf values of 0.27± 0.01 and 0.51± 0.01 for LAM and TDF, respectively. The linear regression analysis data for the calibration plots showed good linear relationship with r²=0.9999 and 0.9996 for LAM and TDF, respectively in the concentration range of 60-210 ng spot¹ for each drug. The method was validated for precision, robustness, specificity and accuracy The limit of detection and quantitation were 20 and 40 ng spot¹, respectively for LAM and 30 and 60 ng spot¹, respectively for TDF. The proposed developed HPTLC method can be applied for identification and quantitative determination of LAM and TDF in bulk drug and pharmaceutical dosage form.
author	CHANDRA,P RATHORE,A. S SATHIYANARAYANAN,L MAHADIK,K. R
author_facet	CHANDRA,P RATHORE,A. S SATHIYANARAYANAN,L MAHADIK,K. R
author_sort	CHANDRA,P
title	APPLICATION OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF LAMIVUDINE AND TENOFOVIR DISOPROXIL FUMARATE IN PHARMACEUTICAL DOSAGE FORM
title_short	APPLICATION OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF LAMIVUDINE AND TENOFOVIR DISOPROXIL FUMARATE IN PHARMACEUTICAL DOSAGE FORM
title_full	APPLICATION OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF LAMIVUDINE AND TENOFOVIR DISOPROXIL FUMARATE IN PHARMACEUTICAL DOSAGE FORM
title_fullStr	APPLICATION OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF LAMIVUDINE AND TENOFOVIR DISOPROXIL FUMARATE IN PHARMACEUTICAL DOSAGE FORM
title_full_unstemmed	APPLICATION OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF LAMIVUDINE AND TENOFOVIR DISOPROXIL FUMARATE IN PHARMACEUTICAL DOSAGE FORM
title_sort	application of high-performance thin-layer chromatographic method for the simultaneous determination of lamivudine and tenofovir disoproxil fumarate in pharmaceutical dosage form
publisher	Sociedad Chilena de Química
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000200017
work_keys_str_mv	AT chandrap applicationofhighperformancethinlayerchromatographicmethodforthesimultaneousdeterminationoflamivudineandtenofovirdisoproxilfumarateinpharmaceuticaldosageform AT rathoreas applicationofhighperformancethinlayerchromatographicmethodforthesimultaneousdeterminationoflamivudineandtenofovirdisoproxilfumarateinpharmaceuticaldosageform AT sathiyanarayananl applicationofhighperformancethinlayerchromatographicmethodforthesimultaneousdeterminationoflamivudineandtenofovirdisoproxilfumarateinpharmaceuticaldosageform AT mahadikkr applicationofhighperformancethinlayerchromatographicmethodforthesimultaneousdeterminationoflamivudineandtenofovirdisoproxilfumarateinpharmaceuticaldosageform
_version_	1718445447602765824

APPLICATION OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF LAMIVUDINE AND TENOFOVIR DISOPROXIL FUMARATE IN PHARMACEUTICAL DOSAGE FORM

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