SENSITIVE AND SELECTIVE EXTRACTION FREE ION-PAIR COMPLEXOMETRIC DETERMINATION OF DOMPERIDONE IN PHARMACEUTICALS AND IN SPIKED HUMAN URINE

Domperidone (DOM) is a drug used as an antiemetic and to control gastrointestinal effects of dopaminergic drugs in the management of perkinsonism. Two simple, rapid, sensitive and selective extraction free spectrophotometric methods have been developed for the assay of DOM in pure drug, in pharmaceu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	ZENITA,O, BASAVAIAH,K, VINAY,K B
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2011
Materias:	Domperidone Assay Spectrophotometry Sulphonphthalein dyes Pharmaceuticals Spiked urine
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000200021
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-97072011000200021
record_format	dspace
spelling	oai:scielo:S0717-970720110002000212011-09-01SENSITIVE AND SELECTIVE EXTRACTION FREE ION-PAIR COMPLEXOMETRIC DETERMINATION OF DOMPERIDONE IN PHARMACEUTICALS AND IN SPIKED HUMAN URINEZENITA,OBASAVAIAH,KVINAY,K B Domperidone Assay Spectrophotometry Sulphonphthalein dyes Pharmaceuticals Spiked urine Domperidone (DOM) is a drug used as an antiemetic and to control gastrointestinal effects of dopaminergic drugs in the management of perkinsonism. Two simple, rapid, sensitive and selective extraction free spectrophotometric methods have been developed for the assay of DOM in pure drug, in pharmaceuticals and in spiked human urine sample. The methods are based on the formation of yellow ion-pair complexes between DOM and two sulphonphthalein dyes viz., thymol blue (method A) and bromothymol blue (method B) in acetone and dichloromethane medium. The complexes showed absorption maxima at 390 nm and 410 nm in method A and method B, respectively. Reaction conditions were optimized to obtain the maximum color intensity. The absorbance was found to increase linearly with increase in concentration of DOM, which was corroborated by the calculated correlation values of 0.9991 in method A and 0.9986 in method B. The systems obeyed Beer's law over the concentration range of 1.25 - 20 µg mL-1 in both the methods. The molar absorptivity values are calculated to be 1.53 x 10(4) and 2.06 X 10(4) L mol¹ cm¹ for method A and method B, respectively and the corresponding Sandell sensitivity values are 0.028 and 0.021 µg cm². The composition of the ion-pairs was found to be 1 : 1 by Job's method and the conditional stability constant (Kf) of the complexes have been calculated. The proposed methods were applied successfully to the determination of DOM in tablets as well as in spiked urine sample with good accuracy and precision.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.56 n.2 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000200021en10.4067/S0717-97072011000200021
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Domperidone Assay Spectrophotometry Sulphonphthalein dyes Pharmaceuticals Spiked urine
spellingShingle	Domperidone Assay Spectrophotometry Sulphonphthalein dyes Pharmaceuticals Spiked urine ZENITA,O BASAVAIAH,K VINAY,K B SENSITIVE AND SELECTIVE EXTRACTION FREE ION-PAIR COMPLEXOMETRIC DETERMINATION OF DOMPERIDONE IN PHARMACEUTICALS AND IN SPIKED HUMAN URINE
description	Domperidone (DOM) is a drug used as an antiemetic and to control gastrointestinal effects of dopaminergic drugs in the management of perkinsonism. Two simple, rapid, sensitive and selective extraction free spectrophotometric methods have been developed for the assay of DOM in pure drug, in pharmaceuticals and in spiked human urine sample. The methods are based on the formation of yellow ion-pair complexes between DOM and two sulphonphthalein dyes viz., thymol blue (method A) and bromothymol blue (method B) in acetone and dichloromethane medium. The complexes showed absorption maxima at 390 nm and 410 nm in method A and method B, respectively. Reaction conditions were optimized to obtain the maximum color intensity. The absorbance was found to increase linearly with increase in concentration of DOM, which was corroborated by the calculated correlation values of 0.9991 in method A and 0.9986 in method B. The systems obeyed Beer's law over the concentration range of 1.25 - 20 µg mL-1 in both the methods. The molar absorptivity values are calculated to be 1.53 x 10(4) and 2.06 X 10(4) L mol¹ cm¹ for method A and method B, respectively and the corresponding Sandell sensitivity values are 0.028 and 0.021 µg cm². The composition of the ion-pairs was found to be 1 : 1 by Job's method and the conditional stability constant (Kf) of the complexes have been calculated. The proposed methods were applied successfully to the determination of DOM in tablets as well as in spiked urine sample with good accuracy and precision.
author	ZENITA,O BASAVAIAH,K VINAY,K B
author_facet	ZENITA,O BASAVAIAH,K VINAY,K B
author_sort	ZENITA,O
title	SENSITIVE AND SELECTIVE EXTRACTION FREE ION-PAIR COMPLEXOMETRIC DETERMINATION OF DOMPERIDONE IN PHARMACEUTICALS AND IN SPIKED HUMAN URINE
title_short	SENSITIVE AND SELECTIVE EXTRACTION FREE ION-PAIR COMPLEXOMETRIC DETERMINATION OF DOMPERIDONE IN PHARMACEUTICALS AND IN SPIKED HUMAN URINE
title_full	SENSITIVE AND SELECTIVE EXTRACTION FREE ION-PAIR COMPLEXOMETRIC DETERMINATION OF DOMPERIDONE IN PHARMACEUTICALS AND IN SPIKED HUMAN URINE
title_fullStr	SENSITIVE AND SELECTIVE EXTRACTION FREE ION-PAIR COMPLEXOMETRIC DETERMINATION OF DOMPERIDONE IN PHARMACEUTICALS AND IN SPIKED HUMAN URINE
title_full_unstemmed	SENSITIVE AND SELECTIVE EXTRACTION FREE ION-PAIR COMPLEXOMETRIC DETERMINATION OF DOMPERIDONE IN PHARMACEUTICALS AND IN SPIKED HUMAN URINE
title_sort	sensitive and selective extraction free ion-pair complexometric determination of domperidone in pharmaceuticals and in spiked human urine
publisher	Sociedad Chilena de Química
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000200021
work_keys_str_mv	AT zenitao sensitiveandselectiveextractionfreeionpaircomplexometricdeterminationofdomperidoneinpharmaceuticalsandinspikedhumanurine AT basavaiahk sensitiveandselectiveextractionfreeionpaircomplexometricdeterminationofdomperidoneinpharmaceuticalsandinspikedhumanurine AT vinaykb sensitiveandselectiveextractionfreeionpaircomplexometricdeterminationofdomperidoneinpharmaceuticalsandinspikedhumanurine
_version_	1718445448389197824

SENSITIVE AND SELECTIVE EXTRACTION FREE ION-PAIR COMPLEXOMETRIC DETERMINATION OF DOMPERIDONE IN PHARMACEUTICALS AND IN SPIKED HUMAN URINE

Ejemplares similares