DEVELOPMENT AND VALIDATION OF A SOLID-PHASE EXTRACTION GAS CHROMATOGRAPHY-MASS SPECTROMETRY METHOD FOR THE SIMULTANEOUS QUANTIFICATION OF OPIOID DRUGS IN HUMAN WHOLE BLOOD AND PLASMA

A rapid, selective, sensitive, and specific method is presented to simultaneously quantify morphine, 6-monoacetylmorphine (6-MAM), codeine, heroin, fentanyl, and methadone in human whole blood and plasma. The drugs were extracted with phosphate buffer at pH 6, followed by solid-phase extraction (SPE...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	BRAVO,FELIPE, GONZALEZ,DIEGO, BENITES,JULIO
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2011
Materias:	Opiods gas chromatography mass selective detector solid-phase extraction
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000300017
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-97072011000300017
record_format	dspace
spelling	oai:scielo:S0717-970720110003000172012-01-11DEVELOPMENT AND VALIDATION OF A SOLID-PHASE EXTRACTION GAS CHROMATOGRAPHY-MASS SPECTROMETRY METHOD FOR THE SIMULTANEOUS QUANTIFICATION OF OPIOID DRUGS IN HUMAN WHOLE BLOOD AND PLASMABRAVO,FELIPEGONZALEZ,DIEGOBENITES,JULIO Opiods gas chromatography mass selective detector solid-phase extraction A rapid, selective, sensitive, and specific method is presented to simultaneously quantify morphine, 6-monoacetylmorphine (6-MAM), codeine, heroin, fentanyl, and methadone in human whole blood and plasma. The drugs were extracted with phosphate buffer at pH 6, followed by solid-phase extraction (SPE) and quantification by GC/MS with electron impact ionization using helium as carrier gas. Quantification was performed based on nalorphine as internal standard (IS). The specificity, linearity, intra- and inter-assay precision and accuracy, and extraction recovery were fully evaluated. The limits of detection (LODs) were 0.40 - 7.63 ng/mL for whole blood and 0.80 - 32.00 ng/mL for plasma. The method is fast, simple, and accurate, with the sensitivity and specificity required in forensic and clinical toxicology.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.56 n.3 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000300017en10.4067/S0717-97072011000300017
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Opiods gas chromatography mass selective detector solid-phase extraction
spellingShingle	Opiods gas chromatography mass selective detector solid-phase extraction BRAVO,FELIPE GONZALEZ,DIEGO BENITES,JULIO DEVELOPMENT AND VALIDATION OF A SOLID-PHASE EXTRACTION GAS CHROMATOGRAPHY-MASS SPECTROMETRY METHOD FOR THE SIMULTANEOUS QUANTIFICATION OF OPIOID DRUGS IN HUMAN WHOLE BLOOD AND PLASMA
description	A rapid, selective, sensitive, and specific method is presented to simultaneously quantify morphine, 6-monoacetylmorphine (6-MAM), codeine, heroin, fentanyl, and methadone in human whole blood and plasma. The drugs were extracted with phosphate buffer at pH 6, followed by solid-phase extraction (SPE) and quantification by GC/MS with electron impact ionization using helium as carrier gas. Quantification was performed based on nalorphine as internal standard (IS). The specificity, linearity, intra- and inter-assay precision and accuracy, and extraction recovery were fully evaluated. The limits of detection (LODs) were 0.40 - 7.63 ng/mL for whole blood and 0.80 - 32.00 ng/mL for plasma. The method is fast, simple, and accurate, with the sensitivity and specificity required in forensic and clinical toxicology.
author	BRAVO,FELIPE GONZALEZ,DIEGO BENITES,JULIO
author_facet	BRAVO,FELIPE GONZALEZ,DIEGO BENITES,JULIO
author_sort	BRAVO,FELIPE
title	DEVELOPMENT AND VALIDATION OF A SOLID-PHASE EXTRACTION GAS CHROMATOGRAPHY-MASS SPECTROMETRY METHOD FOR THE SIMULTANEOUS QUANTIFICATION OF OPIOID DRUGS IN HUMAN WHOLE BLOOD AND PLASMA
title_short	DEVELOPMENT AND VALIDATION OF A SOLID-PHASE EXTRACTION GAS CHROMATOGRAPHY-MASS SPECTROMETRY METHOD FOR THE SIMULTANEOUS QUANTIFICATION OF OPIOID DRUGS IN HUMAN WHOLE BLOOD AND PLASMA
title_full	DEVELOPMENT AND VALIDATION OF A SOLID-PHASE EXTRACTION GAS CHROMATOGRAPHY-MASS SPECTROMETRY METHOD FOR THE SIMULTANEOUS QUANTIFICATION OF OPIOID DRUGS IN HUMAN WHOLE BLOOD AND PLASMA
title_fullStr	DEVELOPMENT AND VALIDATION OF A SOLID-PHASE EXTRACTION GAS CHROMATOGRAPHY-MASS SPECTROMETRY METHOD FOR THE SIMULTANEOUS QUANTIFICATION OF OPIOID DRUGS IN HUMAN WHOLE BLOOD AND PLASMA
title_full_unstemmed	DEVELOPMENT AND VALIDATION OF A SOLID-PHASE EXTRACTION GAS CHROMATOGRAPHY-MASS SPECTROMETRY METHOD FOR THE SIMULTANEOUS QUANTIFICATION OF OPIOID DRUGS IN HUMAN WHOLE BLOOD AND PLASMA
title_sort	development and validation of a solid-phase extraction gas chromatography-mass spectrometry method for the simultaneous quantification of opioid drugs in human whole blood and plasma
publisher	Sociedad Chilena de Química
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000300017
work_keys_str_mv	AT bravofelipe developmentandvalidationofasolidphaseextractiongaschromatographymassspectrometrymethodforthesimultaneousquantificationofopioiddrugsinhumanwholebloodandplasma AT gonzalezdiego developmentandvalidationofasolidphaseextractiongaschromatographymassspectrometrymethodforthesimultaneousquantificationofopioiddrugsinhumanwholebloodandplasma AT benitesjulio developmentandvalidationofasolidphaseextractiongaschromatographymassspectrometrymethodforthesimultaneousquantificationofopioiddrugsinhumanwholebloodandplasma
_version_	1718445451878858752

DEVELOPMENT AND VALIDATION OF A SOLID-PHASE EXTRACTION GAS CHROMATOGRAPHY-MASS SPECTROMETRY METHOD FOR THE SIMULTANEOUS QUANTIFICATION OF OPIOID DRUGS IN HUMAN WHOLE BLOOD AND PLASMA

Ejemplares similares