iN-VITRO INTERACTION STUDIES OF VERAPAMIL WITH FLUOROQUINOLONES USING FIRST ORDER DERIVATIVE UV SPECTROPHOTOMETRY AND RP-HPLC

Verapamil, a calcium channel blocker, is classified as a class IV anti-arrhythmic agent. It is used in the treatment of hypertension, as an important therapeutic agent for angina pectoris, ischemic heart disease, hypertension and hypertrophic cardiomyopathy. Many in-vivo studies have been carried ou...

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Autores principales:	SULTANA,NAJMA, SAEED ARAYNE,MUHAMMAD, WAHEED,ABDUL
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2011
Materias:	Verapamil anti-arrhythmic agent angina pectoris hypertrophic cardiomyopathy fluoroquinolone HPLC and UV spectrophotometry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400005
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spelling	oai:scielo:S0717-970720110004000052012-03-06iN-VITRO INTERACTION STUDIES OF VERAPAMIL WITH FLUOROQUINOLONES USING FIRST ORDER DERIVATIVE UV SPECTROPHOTOMETRY AND RP-HPLCSULTANA,NAJMASAEED ARAYNE,MUHAMMADWAHEED,ABDUL Verapamil anti-arrhythmic agent angina pectoris hypertrophic cardiomyopathy fluoroquinolone HPLC and UV spectrophotometry Verapamil, a calcium channel blocker, is classified as a class IV anti-arrhythmic agent. It is used in the treatment of hypertension, as an important therapeutic agent for angina pectoris, ischemic heart disease, hypertension and hypertrophic cardiomyopathy. Many in-vivo studies have been carried out to find out the effects on concurrent use of calcium channel blocker with various groups of drugs. In the present paper an in-vitro approach was adapted to study the interaction of verapamil with commonly administered fluoroquinolones i.e. ciprofloxacin, levofloxacin, norfloxacin and sparfloxacin. The interaction studies were carried out in different simulated body fluids pH ranges from 1-9 at 37 ºC. To perform these studies first-derivative UV spectrophotometric (using zero-crossing technique) and simultaneous RP-HPLC methods were developed and validated. HPLC analysis was conducted on Shim-pack CLC-ODS (6.0 X 150 mm) column. The mobile phase constituted of acetonitrile: water (45:55), whose pH was adjusted to 2.8 and pumped at a flow rate of 1.2 mL min-1 at 230 nm. The results obtained from both methodologies indicated that the availability of verapamil was not affected by simultaneous administration of fluoroquinolones. Hence the two drugs could be safely administered concomitantly.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.56 n.4 20112011-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400005en10.4067/S0717-97072011000400005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Verapamil anti-arrhythmic agent angina pectoris hypertrophic cardiomyopathy fluoroquinolone HPLC and UV spectrophotometry
spellingShingle	Verapamil anti-arrhythmic agent angina pectoris hypertrophic cardiomyopathy fluoroquinolone HPLC and UV spectrophotometry SULTANA,NAJMA SAEED ARAYNE,MUHAMMAD WAHEED,ABDUL iN-VITRO INTERACTION STUDIES OF VERAPAMIL WITH FLUOROQUINOLONES USING FIRST ORDER DERIVATIVE UV SPECTROPHOTOMETRY AND RP-HPLC
description	Verapamil, a calcium channel blocker, is classified as a class IV anti-arrhythmic agent. It is used in the treatment of hypertension, as an important therapeutic agent for angina pectoris, ischemic heart disease, hypertension and hypertrophic cardiomyopathy. Many in-vivo studies have been carried out to find out the effects on concurrent use of calcium channel blocker with various groups of drugs. In the present paper an in-vitro approach was adapted to study the interaction of verapamil with commonly administered fluoroquinolones i.e. ciprofloxacin, levofloxacin, norfloxacin and sparfloxacin. The interaction studies were carried out in different simulated body fluids pH ranges from 1-9 at 37 ºC. To perform these studies first-derivative UV spectrophotometric (using zero-crossing technique) and simultaneous RP-HPLC methods were developed and validated. HPLC analysis was conducted on Shim-pack CLC-ODS (6.0 X 150 mm) column. The mobile phase constituted of acetonitrile: water (45:55), whose pH was adjusted to 2.8 and pumped at a flow rate of 1.2 mL min-1 at 230 nm. The results obtained from both methodologies indicated that the availability of verapamil was not affected by simultaneous administration of fluoroquinolones. Hence the two drugs could be safely administered concomitantly.
author	SULTANA,NAJMA SAEED ARAYNE,MUHAMMAD WAHEED,ABDUL
author_facet	SULTANA,NAJMA SAEED ARAYNE,MUHAMMAD WAHEED,ABDUL
author_sort	SULTANA,NAJMA
title	iN-VITRO INTERACTION STUDIES OF VERAPAMIL WITH FLUOROQUINOLONES USING FIRST ORDER DERIVATIVE UV SPECTROPHOTOMETRY AND RP-HPLC
title_short	iN-VITRO INTERACTION STUDIES OF VERAPAMIL WITH FLUOROQUINOLONES USING FIRST ORDER DERIVATIVE UV SPECTROPHOTOMETRY AND RP-HPLC
title_full	iN-VITRO INTERACTION STUDIES OF VERAPAMIL WITH FLUOROQUINOLONES USING FIRST ORDER DERIVATIVE UV SPECTROPHOTOMETRY AND RP-HPLC
title_fullStr	iN-VITRO INTERACTION STUDIES OF VERAPAMIL WITH FLUOROQUINOLONES USING FIRST ORDER DERIVATIVE UV SPECTROPHOTOMETRY AND RP-HPLC
title_full_unstemmed	iN-VITRO INTERACTION STUDIES OF VERAPAMIL WITH FLUOROQUINOLONES USING FIRST ORDER DERIVATIVE UV SPECTROPHOTOMETRY AND RP-HPLC
title_sort	in-vitro interaction studies of verapamil with fluoroquinolones using first order derivative uv spectrophotometry and rp-hplc
publisher	Sociedad Chilena de Química
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400005
work_keys_str_mv	AT sultananajma invitrointeractionstudiesofverapamilwithfluoroquinolonesusingfirstorderderivativeuvspectrophotometryandrphplc AT saeedaraynemuhammad invitrointeractionstudiesofverapamilwithfluoroquinolonesusingfirstorderderivativeuvspectrophotometryandrphplc AT waheedabdul invitrointeractionstudiesofverapamilwithfluoroquinolonesusingfirstorderderivativeuvspectrophotometryandrphplc
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iN-VITRO INTERACTION STUDIES OF VERAPAMIL WITH FLUOROQUINOLONES USING FIRST ORDER DERIVATIVE UV SPECTROPHOTOMETRY AND RP-HPLC

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